Fluorometric and resonance Rayleigh scattering dual-mode bioprobe for determination of the activity of alkaline phosphatase based on the use of CoOOH nanoflakes and cobalt(II)-dependent DNAzyme-assisted amplification
- 10 Downloads
The authors describe a fluorometric and resonance Rayleigh scattering dual-mode scheme for detection of the activity and inhibition of alkaline phosphatase (ALP). The method utilizes (a) CoOOH nanoflakes, which have high resonance Rayleigh scattering activity and can strongly adsorb ssDNA, and (b) Co(II)-dependent DNAzyme assisted signal amplification. ALP specifically catalyzes the hydrolysis of ascorbic acid-2-phosphate to produce ascorbic acid which reduces CoOOH nanoflakes to Co(II) ion. The Co(II)-dependent DNAzyme is then activated by Co(II) ion, and this results in the cleavage of a substrate labeled with both a fluorophore and a quencher. Following hydrolysis, fluorophore and quencher become separated and the fluorescence measured at excitation/emission wavelengths of 490/518 nm recovers, while the RRS signal at 405 nm decreases. The method works on the 0.2 to 2000 U L−1 ALP activity range, and the detection limit is 0.05 U L−1. The method was used to validate the mechanism of the action of two classical ALP inhibitors (EDTA and Na3VO4). Conceivably, it can also be applied to screen for ALP inhibitors.
KeywordsMolecular beacon Cycle Cobalt oxyhydroxide DNA RRS Inhibitor Metal ion-dependent DNAzymes Na3VO4 Nanosheet Ascorbic acid
The authors sincerely acknowledge the support of the National Natural Science Foundation of China (No. 21675131, 21273174), and the Natural Science Foundation of Chongqing (No. CSTC-2015jcyjB50001).
Compliance with ethical standards
The authors declare that they have no competing interests.
- 6.Xiao T, Sun J, Zhao JH, Wang S, Liu GY, Yang XR (2018) FRET effect between fluorescent Polydopamine nanoparticles and MnO2 Nanosheets and its application for sensitive sensing of alkaline phosphatase. Acs Appl Mater Inter 10(7):6560–6569. https://doi.org/10.1021/acsami.7b18816 CrossRefGoogle Scholar
- 9.Wang HB, Li Y, Chen Y, Zhang ZP, Gan T, Liu YM (2018) Determination of the activity of alkaline phosphatase by using nanoclusters composed of flower-like cobalt Oxyhydroxide and copper nanoclusters as fluorescent probes. Microchim Acta 185(2):102. https://doi.org/10.1007/s00604-017-2622-4 CrossRefGoogle Scholar
- 10.Jiao HP, Chen J, Li WY, Wang FY, Zhou HP, Li YX, Yu C (2014) Nucleic acid-regulated Perylene probe-induced gold nanoparticle aggregation: a new strategy for colorimetric sensing of alkaline phosphatase activity and inhibitor screening. Acs Appl Mater Inter 6(3):1979–1985. https://doi.org/10.1021/am405020b CrossRefGoogle Scholar
- 11.Shen CC, Li XZ, Rasooly A, Guo LY, Zhang KN, Yang MH (2016) A single electrochemical biosensor for detecting the activity and inhibition of both protein kinase and alkaline phosphatase based on phosphate ions induced deposition of redox precipitates. Biosens Bioelectron 85:220–225. https://doi.org/10.1016/j.bios.2016.05.025 CrossRefPubMedGoogle Scholar
- 15.Zeng Y, Ren JQ, Wang SK, Mai JM, Qu B, Zhang Y, Shen AG, Hu JM (2017) Rapid and reliable detection of alkaline phosphatase by a hot spots amplification strategy based on well-controlled assembly on single nanoparticle. Acs Appl Mater Inter 9(35):29547–29553. https://doi.org/10.1021/acsami.7b09336 CrossRefGoogle Scholar
- 16.Liu SG, Li N, Han L, Li LJ, Li NB, Luo HQ (2018) Size-dependent modulation of fluorescence and light scattering: a new strategy for development of ratiometric sensing. Mater Horiz 5(3):454–460. https://doi.org/10.1039/c7mh00872d
- 19.Knirsch KC, Berner NC, Nerl HC, Cucinotta CS, Gholamvand Z, McEvoy N, Wang ZX, Abramovic I, Vecera P, Halik M, Sanvito S, Duesberg GS, Nicolosi V, Hauke F, Hirsch A, Colernan JN, Backes C (2015) Basal-plane functionalization of chemically exfoliated molybdenum disulfide by Diazonium salts. ACS Nano 9(6):6018–6030. https://doi.org/10.1021/acsnano.5b00965 CrossRefPubMedGoogle Scholar
- 24.Li LB, Wang C, Liu KY, Wang YH, Liu K, Lin YQ (2015) Hexagonal cobalt Oxyhydroxide-carbon dots hybridized surface: high sensitive fluorescence turn-on probe for monitoring of ascorbic acid in rat brain following brain ischemia. Anal Chem 87(6):3404–3411. https://doi.org/10.1021/ac5046609 CrossRefPubMedGoogle Scholar
- 28.Huang JY, Zhao L, Lei W, Wen W, Wang YJ, Bao T, Xiong HY, Zhang XH, Wang SF (2018) A high-sensitivity electrochemical Aptasensor of carcinoembryonic antigen based on graphene quantum dots-ionic liquid-Nafion Nanomatrix and DNAzyme-assisted signal amplification strategy. Biosens Bioelectron 99:28–33. https://doi.org/10.1016/j.bios.2017.07.036 CrossRefPubMedGoogle Scholar
- 32.Meng HM, Zhang XB, Yang C, Kuai HL, Mao GJ, Gong L, Zhang WH, Feng SL, Chang JB (2016) Efficient two-photon fluorescence Nanoprobe for turn-on detection and imaging of ascorbic acid in living cells and tissues. Anal Chem 88(11):6057–6063. https://doi.org/10.1021/acs.analchem.6b01352 CrossRefPubMedGoogle Scholar
- 33.Li JY, Si L, Bao JC, Wang ZY, Dai ZH (2017) Fluorescence regulation of poly (thymine)-templated copper nanoparticles via an enzyme-triggered reaction toward sensitive and selective detection of alkaline phosphatase. Anal Chem 89(6):3681–3686. https://doi.org/10.1021/acs.analchem.6b05112 CrossRefPubMedGoogle Scholar