Microchimica Acta

, 186:417 | Cite as

Fe/C magnetic nanocubes with enhanced peroxidase mimetic activity for colorimetric determination of hydrogen peroxide and glucose

  • Fencheng Yang
  • Guodong Jiang
  • Feng Yan
  • Qing ChangEmail author
Original Paper


Iron-carbon (Fe/C) magnetic nanocubes were synthesized by direct pyrolysis of Prussian blue nanocubes under an inert gas atmosphere. They are shown to possess intrinsic peroxidase mimicking activity to catalyze the oxidation of peroxidase substrate N,N-diethyl-p-phenylenediamine sulfate salt to form a purple colored product in the presence of H2O2. The values for Km and Vmax are 74 μM and 46 nmol s−1, respectively. Steady-state kinetic analysis also indicates that the catalysis reaction follows a ping-pong mechanism. Based on these findings, an ultrasensitive colorimetric H2O2 assay was worked. Absorbance (best value measured at 550 nm) increases linearly in the 10 nM to 0.2 mM H2O2 concentration range, and the limit of detection is 1.5 nM. The method was also applied to the quantification of glucose, which is oxidized by glucose oxidase in the coexistence of H2O2. The response covers the 0.1 to 500 μM glucose concentration range, and the limit of detection is 16 nM. The method was applied to the determination of H2O2 in rainwater samples. The glucose assay was used to analyze serum samples, and satisfactory results were obtained. Other attractive features include good chemical activity, low cost, easy storage, and high catalytic efficiency.

Graphical abstract

Schematic presentation of converting Prussian blue nanoparticles into Fe/C magnetic nanocubes by a pyrolysis technique and the use of glucose oxidase and Fe/C magnetic nanocubes to establish a one-step spectrophotometric method for the determination of glucose and hydrogen peroxide.


Magnetic nanocubes Catalytic activity Glucose Hydrogen peroxide DPD oxidation Photometric determination 



This work was supported by grants from the Natural Science Foundation of Hubei Province of China (Nos. 2016CFB505).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3527_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1.33 MB)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Resource and Environment ScienceSouth-Central University for NationalitiesWuhanPeople’s Republic of China
  2. 2.College of Chemistry and Chemical Engineering, Hubei Collaborative Innovation Center for High Efficient Utilization of Solar EnergyHubei University of TechnologyWuhanPeople’s Republic of China

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