Microchimica Acta

, 186:414 | Cite as

Molecularly imprinted polydopamine modified with nickel nanoparticles wrapped with carbon: fabrication, characterization and electrochemical detection of uric acid

  • Yanying Wang
  • Xin Liu
  • Zhiwei Lu
  • Tao Liu
  • Lijun Zhao
  • Fang Ding
  • Ping Zou
  • Xianxiang Wang
  • Qingbiao ZhaoEmail author
  • Hanbing RaoEmail author
Original Paper


An electrochemical sensor is described for determination of uric acid (UA). Carbon-enwrapped nickel nanoparticles (Ni@BC) were coated with polydopamine (PDA) that was molecularly imprinted with UA. The biomass carbon (BC) was synthesized by one-step solid-state pyrolysis from leaves of Firmiana platanifolia. The imprinted polymer was obtained by electrodeposition of DA as the monomer. The amount of monomer, the scan cycles, pH value and adsorption time were optimized. Furthermore, the selectivity of the MIP for UA on a glassy carbon electrode (GCE) was evaluated by selectivity tests. The differential pulse voltammetric responses to UA with and without interferents were consistent. The modified GCE has a linear response in the 0.01–30 μM UA concentration range, and the limit of detection is 8 nM. The MIP electrode was applied to the analysis of UA in urine for which the initial concentrations were determined by the phosphotungstic acid kit. Recoveries ranged from 91.3 to 113.4%, with relative standard deviations between 1.3 and 9.7% (n = 3).

Graphical abstract

Schematic presentation of electrochemical detection of uric acid by molecularly imprinted polydopamine modified with nickel nanoparticles wrapped with carbon (Ni@BC-MIP).


Biomass carbon Molecularly imprinted polymers Electrochemical sensor Differential pulse voltammetry Urine 



This work was supported by the following grant: The Two-Way Support Programs of Sichuan Agricultural University, P. R. China (03570113), the Education Department of Sichuan Province, P. R. China (16ZA0039).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3521_MOESM1_ESM.doc (8.6 mb)
ESM 1 (DOC 8.62 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Yanying Wang
    • 1
  • Xin Liu
    • 1
  • Zhiwei Lu
    • 1
  • Tao Liu
    • 2
  • Lijun Zhao
    • 3
  • Fang Ding
    • 4
  • Ping Zou
    • 1
  • Xianxiang Wang
    • 1
  • Qingbiao Zhao
    • 5
    Email author
  • Hanbing Rao
    • 1
    Email author
  1. 1.College of ScienceSichuan Agricultural UniversityYa’anPeople’s Republic of China
  2. 2.College of Information EngineeringSichuan Agricultural UniversityYa’anPeople’s Republic of China
  3. 3.Ministry of Agriculture and Rural Affairs Laboratory of Risk Assessment for Quality and Safety of Livestock and PoultryChengduPeople’s Republic of China
  4. 4.Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and EngineeringShenzhen UniversityShenzhenPeople’s Republic of China
  5. 5.Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of OptoelectronicsEast China Normal UniversityShanghaiPeople’s Republic of China

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