Preparation of a glassy carbon electrode modified with reduced graphene oxide and overoxidized electropolymerized polypyrrole, and its application to the determination of dopamine in the presence of ascorbic acid and uric acid
- 39 Downloads
This paper presents a method for the preparation of a graphene-based hybrid composite film by electrodeposition of reduced graphene oxide and overoxidized electropolymerized polypyrrole onto a glassy carbon electrode (GCE) using cyclic voltammetry. The morphology of the hybrid composite film was characterized by scanning electron microscopy. The electrochemical activity of the modified GCE was studied by cyclic voltammetry using the negatively charged redox probe Fe(CN)63− and the positively charged redox probe Ru(NH3)63+. The modified GCE displays excellent electrocatalytic activity for dopamine (DA) and uric acid (UA), but electrostatically repulses ascorbate anion under physiological pH conditions. The voltammetric response to DA is linear in the 2.0 μM to 160 μM concentration range even in the presence of 1.0 mM ascorbic acid and 0.1 mM of UA. The detection limit is 0.5 μM. The amperometric response to DA (best measured at 0.22 V vs. Ag/AgCl) extends from 0.4 μM to 517 μM and has a 0.2 μM detection limit.
KeywordsGraphene Polypyrrole Hybrid composite Nanocomposite Modified electrode Neurotransmitter Electrochemical sensor
This work was supported by the National Natural Science Foundation of China (No.21305106) and Shaanxi Province Natural Science Foundation of China (No.2019JM-469).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 1.Janezic S, Threlfell S, Dodson PD, Dowie MJ, Taylor TN, Potgieter D, Parkkinen L, Senior SL, Anwar S, Ryan B, Deltheil T, Kosillo P, Cioroch M, Wagner K, Ansorge O, Bannerman DM, Bolam JP, Magill PJ, Cragg SJ, Wade-Martins R (2013) Deficits in dopaminergic transmission precede neuron loss and dysfunction in a new Parkinson model. Proc Natl Acad Sci U S A 110:E4016–E4025CrossRefGoogle Scholar
- 4.Zhang D, Li L, Ma W, Chen X, Zhang Y (2017) Electrodeposited reduced graphene oxide incorporating polymerization of l-lysine on electrode surface and its application in simultaneous electrochemical determination of ascorbic acid, dopamine and uric acid. Mat Sci Eng C-Mater 70:241–249CrossRefGoogle Scholar
- 6.Tavakolian E, Tashkhourian J (2018) Sonication-assisted preparation of a nanocomposite consisting of reduced graphene oxide and CdSe quantum dots, and its application to simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid. Microchim Acta 185:456–463CrossRefGoogle Scholar
- 33.Mathew G, Dey P, Das R, Chowdhury SD, Paul Das M, Veluswamy P, Neppolian B, Das J (2018) Direct electrochemical reduction of hematite decorated graphene oxide (alpha-Fe2O3@erGO) nanocomposite for selective detection of Parkinson's disease biomarker. Biosens Bioelectron 115:53–60CrossRefGoogle Scholar
- 34.Zhang C, Ren J, Zhou J, Cui M, Li N, Han B, Chen Q (2018) Facile fabrication of a 3,4,9,10-perylene tetracarboxylic acid functionalized graphene-multiwalled carbon nanotube-gold nanoparticle nanocomposite for highly sensitive and selective electrochemical detection of dopamine. Analyst 143:3075–3084CrossRefGoogle Scholar