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Microchimica Acta

, 186:406 | Cite as

Fluorometric determination of okadaic acid using a truncated aptamer

  • Raja Chinnappan
  • Razan AlZabn
  • Tanveer Ahmad Mir
  • Mamoun Bader
  • Mohammed ZourobEmail author
Original Paper
  • 36 Downloads

Abstract

Okadaic acid (OKA), a marine toxin produced by dinoflagellates, is responsible for most human diarrhetic shellfish poisoning-associated health disorders. A competitive displacement assay for OKA is described here. An OKA-binding aptamer was truncated with two sequences, one labeled with 6-carboxyfluorescein (FAM), and one with a quencher. On addition of OKA, it will bind to the aptamer and green fluorescence pops up because label and quencher become spatially separated. One of the truncated aptamers exhibis an excellent binding capability (Kd 2.77 nM) for OKA compared to its full-length aptamer (526 nM). The selectivity of the assay was proven by the successful fluorometric determination of OKA in the presence of common diarrhoetic toxins and in shellfish extracts. The detection limit is as low as 39 pg·mL−1.

Graphical abstract

Schematic representation of the competitive displacement assay for okadaic acid (OKA). The OKA-binding aptamer is truncated with two parts, one labeled with 6-carboxyfluorescein (FAM), and one with a quencher. On addition of OKA, green fluorescence pops up because label and quencher become spatially separated.

Keywords

Okadaic acid Truncated aptamer Toxins Aptasensor Shellfish poisoning Fluorescence assay Fluorescence quenching Food poisoning Aptamer binding probe and dinoflagellates toxins 

Notes

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3517_MOESM1_ESM.docx (132 kb)
ESM 1 (DOCX 132 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Raja Chinnappan
    • 1
  • Razan AlZabn
    • 1
  • Tanveer Ahmad Mir
    • 1
  • Mamoun Bader
    • 1
  • Mohammed Zourob
    • 1
    • 2
    Email author
  1. 1.Department of ChemistryAlfaisal UniversityRiyadhSaudi Arabia
  2. 2.King Faisal Specialist Hospital and Research CenterRiyadhSaudi Arabia

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