Magnetic cucurbituril-based hypercrosslinked polymers for efficient enrichment of ubiquitin
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The design and preparation of magnetic cucurbituril hypercrosslinked with polymers are described. The materials have a large specific surface, abundant mesopores and cavities, and display superparamagnetism. They were applied to the enrichment of ubiquitinated peptides from standard protein digests. Following desorption with 0.15% TFA, the peptides were quantified by MALDI-TOF MS. The method has a detection limit of 2 fmol·μL−1 and a mass ratio selectivity of 1:5000 as shown for ubiquitin and bovine serum albumin. The materials enable selective capture of ubiquitinated peptides from genuine samples comprising of oyster mushroom and human serum. This demonstrates their potential for the analysis of low-level ubiquitin in complex samples.
KeywordsProteomics Trypsin Solid phase extraction MALDI-TOF MS Adsorbent Preconcentration Ovalbumin Bovine serum albumin Human serum Oyster mushroom
This work was financially supported by State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, China (2019-4).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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