Microchimica Acta

, 186:510 | Cite as

Magnetic cucurbit[6]uril-based hypercrosslinked polymers for efficient enrichment of ubiquitin

  • Dandan Jiang
  • Zheng Li
  • Qiong JiaEmail author
Original Paper


The design and preparation of magnetic cucurbit[6]uril hypercrosslinked with polymers are described. The materials have a large specific surface, abundant mesopores and cavities, and display superparamagnetism. They were applied to the enrichment of ubiquitinated peptides from standard protein digests. Following desorption with 0.15% TFA, the peptides were quantified by MALDI-TOF MS. The method has a detection limit of 2 fmol·μL−1 and a mass ratio selectivity of 1:5000 as shown for ubiquitin and bovine serum albumin. The materials enable selective capture of ubiquitinated peptides from genuine samples comprising of oyster mushroom and human serum. This demonstrates their potential for the analysis of low-level ubiquitin in complex samples.

Graphical abstract

Schematic presentation for the synthesis of magnetic cucurbit[6]urils hypercrosslinked polymers (MagCB[6]-HCPs).


Proteomics Trypsin Solid phase extraction MALDI-TOF MS Adsorbent Preconcentration Ovalbumin Bovine serum albumin Human serum Oyster mushroom 



This work was financially supported by State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, China (2019-4).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3507_MOESM1_ESM.docx (319 kb)
ESM 1 (DOCX 319 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of ChemistryJilin UniversityChangchunChina

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