Microchimica Acta

, 186:403 | Cite as

Antimony-doped tin oxide nanoparticles as peroxidase mimics for paper-based colorimetric detection of glucose using smartphone read-out

  • Yana Li
  • Jun Sun
  • Wei Mao
  • Sheng TangEmail author
  • Kai Liu
  • Tong Qi
  • Huimin Deng
  • Wei ShenEmail author
  • Lizhuang Chen
  • Luming PengEmail author
Original Paper


Antimony-doped tin oxide nanoparticles (ATO NPs) were loaded on a filter paper where they act as a peroxidase mimic without electrochemical or photochemical assistance. The peroxidase mimicking activity is distinctly improved compared to most known nanomaterials and to natural horseradish peroxidase. The catalytic properties depend on the amount of antimony doped into the ATO NPs. A glucose assay was worked out that is based on (a) the oxidation of glucose by glucose oxidase under formation of H2O2, (b) the oxidation of 3,3,5,5-tetramethybenzidine (TMB) catalyzed by ATO NPs to form blue-green colored oxidized TMB on the surface of the paper. The coloration was analyzed with a smartphone. The method has a 21 μM limit of detection and a linear range that extends from 0.5 to 80 mM.

Graphical abstract

Antimony-doped tin oxide nanoparticles (ATO NPs) combined with 3,3,5,5-tetramethybenzidine (TMB) and triethylamine were coated on the filter paper. After addition of sample solution, the blue-green colored oxidized TMB was generated and recorded by a digital camera.


Peroxidase-activity Paper-based device Smartphone colorimetry Antimony doping Enzyme mimic H2O2 



This work was supported by National Natural Science Foundation of China (No. 21705060, 21605105 and 21573103), Natural Science Foundation of Jiangsu Province, China (No. BK20170570), College Natural Science Foundation of Jiangsu Province, China (No. 17KJB150014) and Six Talent Peaks Project in Jiangsu Province, China (No. SWYY-021).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3506_MOESM1_ESM.doc (10.3 mb)
ESM 1 (DOC 10.2 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Environmental and Chemical EngineeringJiangsu University of Science and TechnologyJiangsu ProvincePeople’s Republic of China
  2. 2.China National Tobacco Quality Supervision and Test CenterNo. 2 Fengyang Street, High and New Technology Industries Development ZoneZhengzhouChina
  3. 3.Key Laboratory of Mesoscopic Chemistry of MOE and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical EngineeringNanjing UniversityNanjingChina

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