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Microchimica Acta

, 186:357 | Cite as

Gold-platinum nanoflowers as a label and as an enzyme mimic for use in highly sensitive lateral flow immunoassays: application to detection of rabbit IgG

  • Jing Zhang
  • Qingcai Yu
  • Wanwei Qiu
  • Kun Li
  • Lisheng QianEmail author
  • Xueji ZhangEmail author
  • Guodong LiuEmail author
Original Paper
  • 71 Downloads

Abstract

The authors describe the preparation of gold-platinum nanoflower (AuPt NFs) and show that they can be simultaneously used as a label and as an enzyme mimic in lateral flow immunoassays (LFIs). The AuPt NFs were prepared by growing Pt nanowires on the surface of gold nanoparticle. The assay involves the capture of target proteins (here: rabbit IgG as a model analyte) by the immobilized capture antibody, and by using AuPt NF-labeled secondary antibody. The AuPt NFs are thus captured by the test zone and produce a characteristic black band for visual detection of the antigen (IgG). The coloration of the test line can be further enhanced by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole which is catalytically oxidized by the captured Pt nanowires on the AuPt NF and produce a red coloration. Quantitative results were obtained by reading the test line intensities with a portable strip reader. The LFI has a 5 pg mL-1 detection limit for IgG under optimized experimental conditions. This is 100 times lower than that of the conventional AuNP-based LFI. Conceivably, this assay has a wide scope in that it may be applied to numerous other targets for which appropriate antibodies are available.

Graphical abstract

Gold-platinum nanoflowers are used as a label and as an enzyme mimic in a highly sensitive lateral flow immunoassay for IgG. The detection limit of gold-platinum nanoflower-based lateral flow assay is 100 times lower than that of the conventional gold nanopaticle-based lateral flow assay.

Keywords

Gold nanoparticles Platinum; enzyme mimic Nanoflower Lateral flow immunoassay 

Notes

Acknowledgements

This research was supported by the National Institute of Health, Centers of Biomedical Research Excellence (NIH, COBRE, Grant number: P20 GM109024), the National Natural Science Foundation of China (Grant No. 31700735) and the National Natural Science Foundation of Anhui Province (Grant No: 1908085 MB54, 1808085QH264). Its contents are solely the responsibility of the authors, and do not necessarily represent the official views of the NIH. G. Liu acknowledges the support from the Wanjiang scholars in Anhui Province, China.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3464_MOESM1_ESM.doc (4.7 mb)
ESM 1 (DOC 4765 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Biomedical and Health Science, School of Life and Health ScienceAnhui Science and Technology UniversityFengyangChina
  2. 2.Research Center for Bioengineering and Sensing TechnologyUniversity of Science & Technology BeijingBeijingPeople’s Republic of China
  3. 3.Department of Chemistry and BiochemistryNorth Dakota State UniversityFargoUSA

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