A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma
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The authors describe a versatile aptasensing scheme based on the use of polypyrrole nanoparticles (PPyNPs) and DNA-silver nanoclusters (DNA-AgNCs) for multiple target detection. The DNA-AgNCs consist of two functional domains, viz. (a) a nucleation domain for attaching the metal core of the nanoclusters, and (b) a recognition domain which consists of a single-stranded aptamer. In the absence of analytes, the single-strand recognition domain will be absorbed onto the surface of the PPyNPs through π stacking and hydrophobic interactions. As a result, the red fluorescence of the DNA-AgNCs (with excitation/emission peaks at 535/625 nm) is quenched by the PPyNPs. On introducing the analytes, the DNA-AgNCs will bind them. This leads to the desorption of DNA-AgNCs and the recovery of the red fluorescence. Based on the above strategy, a versatile, sensitive and selective aptasensor was established for detection of adenosine, thrombin and interferon-gamma. The method was applied to the detection of the above targets in (spiked) serum samples and gave satisfactory results, with detection limit of 0.58 nM for IFN-γ, 0.39 nM for adenosine, and 2.2 nM for thrombin. The use of PPyNPs results in uniquely low non-specific absorption and in improved analytical results in case of real-sample analysis when compared to previously reported methods.
KeywordsAptasensor DNA-silver nanoclusters Steric hindrance Polypyrrole nanoparticles Hybrid system Nanosensor Adenosine Thrombin Interferon-gamma Nucleic acid Fluorescence
This work was supported by the National Natural Science Foundation of China (Nos. 81773681, 81572081 and 81273480). B.Z.L. acknowledges funding from Postgraduate Research & Practice Innovation Program of Jiangsu Province (JX22013405).
Compliance with ethical standards
The authors declare no competing financial or non-financial interest.
- 6.Zheng F, Zhang P, Xi Y, Chen J, Li L, Zhu J (2015) Aptamer/Graphene quantum dots nanocomposite capped fluorescent mesoporous silica nanoparticles for intracellular drug delivery and real-time monitoring of drug release. Anal Chem 87(23):11739–11745. https://doi.org/10.1021/acs.analchem.5b03131 CrossRefPubMedGoogle Scholar
- 13.Liu X, Wang F, Aizen R, Yehezkeli O, Willner I (2013) Graphene oxide/nucleic-acid-stabilized silver nanoclusters: functional hybrid materials for optical aptamer sensing and multiplexed analysis of pathogenic DNAs. J Am Chem Soc 135(32):11832–11839. https://doi.org/10.1021/ja403485r CrossRefPubMedGoogle Scholar
- 22.Li B, Chen Y, Wang J, Lu Q, Zhu W, Xu L, Shen X, Luo J, Zhu C, Li X, Hong J, Zhou X (2018) DNA-silver nanoclusters/polypyrrole nanoparticles: a label-free and enzyme-free platform for multiplexed transcription factors detection. Sensors Actuators B Chem 274:481–490. https://doi.org/10.1016/j.snb CrossRefGoogle Scholar
- 27.Jiang J, He Y, Yu X, Zhao J, Cui H (2013) A homogeneous hemin/G-quadruplex DNAzyme based turn-on chemiluminescence aptasensor for interferon-gamma detection via in-situ assembly of luminol functionalized gold nanoparticles, deoxyribonucleic acid, interferon-gamma and hemin. Anal Chim Acta 791:60–64. https://doi.org/10.1016/j.aca CrossRefPubMedGoogle Scholar
- 30.Farid S, Meshik X, Choi M, Mukherjee S, Lan Y, Parikh D, Poduri S, Baterdene U, Huang C, Wang YY, Burke P, Dutta M, Stroscio MA (2015) Detection of interferon gamma using graphene and aptamer based FET-like electrochemical biosensor. Biosens Bioelectron 71:294–299. https://doi.org/10.1016/j.bios CrossRefPubMedGoogle Scholar