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Microchimica Acta

, 186:287 | Cite as

Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide

  • Dongkui Liu
  • Yanbo Zeng
  • Guobao Zhou
  • Xing Lu
  • Dongwei Miao
  • Yiwen YangEmail author
  • Yunyun Zhai
  • Jian Zhang
  • Zulei Zhang
  • Hailong Wang
  • Lei LiEmail author
Original Paper
  • 64 Downloads

Abstract

The authors describe a fluorometric assay for cardiac myoglobin (Mb), a marker for myocardial infarction. An Mb-binding aptamer was labeled with pyrene and adsorbed on the surface of graphene oxide (GO) via noncovalent and reversible binding forces. This causes the fluorescence of pyrene (best measured at excitation/emission wavelengths of 275/376 nm) to be quenched. However, fluorescence is restored on addition of pyrene due to the strong affinity between Mb and aptamer which causes its separation from GO. Fluorescence increases linearly in the 5.6–450 pM Mb concentration range, and the lower detection limit is 3.9 pM (S/N = 3). The assay was applied to the determination of cardiac Mb in spiked serum, and satisfactory results were obtained.

Graphical abstract

Schematic presentation of the detection of Mb (cardiac myoglobin) by using a fluorometric method based on pyrene-modified anti-Mb aptamer and GO (graphene oxide) through fluorescence quenching and subsequent recovery.

Keywords

Chemical modifications PMAMA probe FRET Fluorophore Fluorescent recovery 

Notes

Acknowledgements

The Authors acknowledge the National Natural Science Foundation of China (No. 21677060 and 51503079), the Public Welfare Research Project of Zhejiang Province (No. LGF18B050004), the Natural Science Foundation of Zhejiang Province (No.s LY16B050007 and LQ19B050002), the Program for Science and Technology of Zhejiang Province (No. 2018C37076) and the Science and Technology Plan Project of Jiaxing City, China (No. 2017AY33034 and 2018AY11002) for funding this research.

Compliance with ethical standards

Human sera sample used in this work was obtained from a healthy volunteer. This study was performed on the consent of the healthy volunteer and approved by the Medical Ethics Committee of Xin’an International Hospital (Jiaxing, China). The authors declare that they have no competing interests.

Supplementary material

604_2019_3385_MOESM1_ESM.docx (159 kb)
ESM 1 (DOCX 159 kb)

References

  1. 1.
    Wu AH, Laios I, Green S, Gornet TG, Wong SS, Parmley L, Tonnesen AS, Plaisier B, Orlando R (1994) Immunoassays for serum and urine myoglobin: myoglobin clearance assessed as a risk factor for acute renal failure. Clin Chem 40(5):796–802PubMedGoogle Scholar
  2. 2.
    Giaretta N, Di GA, Lippert M, Parente A, Di MA (2013) Myoglobin as marker in meat adulteration: a UPLC method for determining the presence of pork meat in raw beef burger. Food Chem 141(3):1814–1820CrossRefGoogle Scholar
  3. 3.
    Naveena BM, Faustman C, Tatiyaborworntham N, Yin S, Ramanathan R, Mancini RA (2010) Detection of 4-hydroxy-2-nonenal adducts of Turkey and chicken myoglobins using mass spectrometry. Food Chem 122(3):836–840CrossRefGoogle Scholar
  4. 4.
    Shorie M, Kumar V, Kaur H, Singh K, Tomer VK, Sabherwal P (2018) Plasmonic DNA hotspots made from tungsten disulfide nanosheets and gold nanoparticles for ultrasensitive aptamer-based SERS detection of myoglobin. Microchim Acta 185(3):1–8CrossRefGoogle Scholar
  5. 5.
    El-Said WA, Fouad DM, El-Safty SA (2016) Ultrasensitive label-free detection of cardiac biomarker myoglobin based on surface-enhanced Raman spectroscopy. Sensors Actuators B Chem 228:401–409CrossRefGoogle Scholar
  6. 6.
    Gnedenko OV, Mezentsev YV, Molnar AA, Lisitsa AV, Ivanov AS, Archakov AI (2013) Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor. Anal Chim Acta 759:105–109CrossRefGoogle Scholar
  7. 7.
    Yang Z, Wang H, Dong X, Yan H, Lei C, Luo Y (2017) Giant magnetoimpedance based immunoassay for cardiac biomarker myoglobin. Anal Methods 9(24):3636–3642CrossRefGoogle Scholar
  8. 8.
    Suprun EV, Shilovskaya AL, Lisitsa AV, Bulko TV, Shumyantseva VV, Archakov AI (2011) Electrochemical immunosensor based on metal nanoparticles for cardiac myoglobin detection in human blood plasma. Electroanalysis 23(5):1051–1057CrossRefGoogle Scholar
  9. 9.
    Singh S, Tuteja SK, Sillu D, Deep A, Suri CR (2016) Gold nanoparticles-reduced graphene oxide based electrochemical immunosensor for the cardiac biomarker myoglobin. Microchim Acta 183(5):1729–1738CrossRefGoogle Scholar
  10. 10.
    Pur MRK, Hosseini M, Faridbod F, Ganjali MR (2017) Highly sensitive label-free electrochemiluminescence aptasensor for early detection of myoglobin, a biomarker for myocardial infarction. Microchim Acta 184(9):3529–3537CrossRefGoogle Scholar
  11. 11.
    Yue Q, Song Z (2006) Assay of femtogram level nitrite in human urine using luminol–myoglobin chemiluminescence. Microchem J 84(1–2):10–13CrossRefGoogle Scholar
  12. 12.
    Abnous K, Danesh NM, Sarreshtehdar Emrani A, Ramezani M, Taghdisi SM (2016) A novel fluorescent aptasensor based on silica nanoparticles, PicoGreen and exonuclease III as a signal amplification method for ultrasensitive detection of myoglobin. Anal Chim Acta 917:71–78CrossRefGoogle Scholar
  13. 13.
    Zhang X, Kong X, Fan W, Du X (2011) Iminodiacetic acid-functionalized gold nanoparticles for optical sensing of myoglobin via Cu2+ coordination. Langmuir 27(10):6504–6510CrossRefGoogle Scholar
  14. 14.
    Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249(4968):505–510CrossRefGoogle Scholar
  15. 15.
    Tombelli S, Minunni M, Mascini M (2005) Analytical applications of aptamers. Biosens Bioelectron 20(12):2424–2434CrossRefGoogle Scholar
  16. 16.
    Zhang G, Liu Z, Wang L, Guo Y (2016) Electrochemical aptasensor for myoglobin-specific recognition based on porphyrin functionalized graphene-conjugated gold nanocomposites. Sensors 16(11):1803/1–1803/12Google Scholar
  17. 17.
    Kumar V, Shorie M, Ganguli AK, Sabherwal P (2015) Graphene-CNT nanohybrid aptasensor for label free detection of cardiac biomarker myoglobin. Biosens Bioelectron 72:56–60CrossRefGoogle Scholar
  18. 18.
    Nia NG, Azadbakht A (2018) Nanostructured aptamer-based sensing platform for highly sensitive recognition of myoglobin. Microchim Acta 185(7):1–10CrossRefGoogle Scholar
  19. 19.
    Varghese N, Mogera U, Govindaraj A, Das A, Maiti PK, Sood AK, Rao CNR (2009) Binding of DNA nucleobases and nucleosides with graphene. ChemPhysChem 10(1):206–210CrossRefGoogle Scholar
  20. 20.
    Swathi RS, Sebastian KL (2009) Long range resonance energy transfer from a dye molecule to graphene has (distance)−4 dependence. J Chem Phys 130(8):086101/1–086101/3CrossRefGoogle Scholar
  21. 21.
    Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184(7):1899–1914CrossRefGoogle Scholar
  22. 22.
    Bai Y, Feng F, Zhao L, Chen Z, Wang H, Duan Y (2014) A turn-on fluorescent aptasensor for adenosine detection based on split aptamers and graphene oxide. Analyst 139(8):1843–1846CrossRefGoogle Scholar
  23. 23.
    Zhu Y, Cai Y, Xu L, Zheng L, Wang L, Qi B, Xu C (2015) Building an aptamer/graphene oxide FRET biosensor for one-step detection of bisphenol a. ACS Appl Mater Interfaces 7(14):7492–7496CrossRefGoogle Scholar
  24. 24.
    Lu CH, Yang HH, Zhu CL, Chen X, Chen GN (2009) A graphene platform for sensing biomolecules. Angew Chem 48(26):4785–4787CrossRefGoogle Scholar
  25. 25.
    Yao C, Kraatz HB, Steer RP (2005) Photophysics of pyrene-labelled compounds of biophysical interest. Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology 4(2):191–199CrossRefGoogle Scholar
  26. 26.
    Winnik FM (1993) Photophysics of preassociated pyrenes in aqueous polymer solutions and in other organized media. Chem Rev 93(2):587–614CrossRefGoogle Scholar
  27. 27.
    Kapf A, Albrecht M (2018) Discrimination of proteins through interaction with pyrene-labelled polymer aggregates. J Mater Chem B 6(41):6599–6606CrossRefGoogle Scholar
  28. 28.
    Wang Q, Liu F, Yang X, Wang K, Wang H, Deng X (2015) Sensitive point-of-care monitoring of cardiac biomarker myoglobin using aptamer and ubiquitous personal glucose meter. Biosens Bioelectron 64:161–164CrossRefGoogle Scholar
  29. 29.
    Taghdisi SM, Danesh NM, Ramezani M, Emrani AS, Abnous K (2016) A novel electrochemical aptasensor based on Y-shape structure of dual-aptamer-complementary strand conjugate for ultrasensitive detection of myoglobin. Biosens Bioelectron 80:532–537CrossRefGoogle Scholar
  30. 30.
    Anjum S, Qi W, Gao W, Zhao J, Hanif S, Aziz Ur R, Xu G (2015) Fabrication of biomembrane-like films on carbon electrodes using alkanethiol and diazonium salt and their application for direct electrochemistry of myoglobin. Biosens Bioelectron 65:159–165CrossRefGoogle Scholar
  31. 31.
    Shumyantseva VV, Bulko TV, Sigolaeva LV, Kuzikov AV, Archakov AI (2016) Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine for selective recognition and direct electrochemical detection of myoglobin. Biosens Bioelectron 86:330–336CrossRefGoogle Scholar
  32. 32.
    Wang Q, Yang X, Yang X, Liu F, Wang K (2015) Visual detection of myoglobin via G-quadruplex DNAzyme functionalized gold nanoparticles-based colorimetric biosensor. Sensors Actuators B Chem 212:440–445CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Biological, Chemical Sciences and EngineeringJiaxing UniversityJiaxingChina
  2. 2.School of Petrochemical EngineeringChangzhou UniversityChangzhouChina

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