Microchimica Acta

, 186:263 | Cite as

A fluorometric displacement assay for adenosine triphosphate using layered cobalt(II) double hydroxide nanosheets

  • Jingjing Liu
  • Xiao Xu
  • Zhitao Chen
  • Renfu Li
  • Longtian KangEmail author
  • Jiannian YaoEmail author
Original Paper


A turn-on fluorometric method is described for the determination of adenosine-5′-triphosphate (ATP). It is based on the displacement of a dye-labeled oligonucleotide from a cobalt(II) based layered double hydroxide (LDH). Due to the electrostatic and ligand exchange interaction, the FAM-labeled DNA is readily adsorbed on the LDH. This leads to complete and fast quenching of the green fluorescence of the label. However, on addition of ATP, the DNA is detached from the LDH because of the stronger affinity of ATP for LDH. This results in the restoration of the green fluorescence. The effect was used to design a sensitive assay that has a linear response in the 0.5–100 μM ATP concentration range and a 0.23 μM lower detection limit. It was applied to the determination of ATP in spiked serum samples.

Graphical abstract

Schematic presentation of a fluorometric ATP assay based on the displacement of a dye-labeled oligonucleotide from a layered double hydroxide (LDH).


Dye-labeled DNA Phosphate-containing metabolites Two-dimensional layered materials Quenching mechanism Serum analysis 



The authors gratefully acknowledge the financial from the National Natural Science Foundation of China (21705150, 21473204), the Natural Science Foundation of Fujian Province (2015 J01070), and the Science and Technology Planning Project of Fujian Province (2014H2008).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3371_MOESM1_ESM.doc (732 kb)
ESM 1 (DOC 732 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhouPeople’s Republic of China
  2. 2.School of Ocean and Biochemical EngineeringFuqing Branch of Fujian Normal UniversityFuqingPeople’s Republic of China
  3. 3.Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of ChemistryChinese Academy of SciencesBeijingPeople’s Republic of China

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