Open-tubular capillary electrochromatography with β-cyclodextrin-functionalized magnetic nanoparticles as stationary phase for enantioseparation of dansylated amino acids
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Magnetic nanoparticles (MNPs) modified with β-cyclodextrin and mono-6-deoxy-6-(1-methylimidazolium)-β-cyclodextrin tosylate (an ionic liquid), which called MNP-β-CD and MNP-β-CD-IL, were coated into the capillary inner wall. Compared to an uncoated capillary, the new systems show good reproducibility and durability. The systems based on the use of MNP-β-CD or MNP-β-CD-IL as stationary phases were established for enantioseparation of Dns-modified amino acids. Improved resolutions were obtained for both CEC systems. Primary parameters such as running buffer pH value and applied voltage were systematically optimized in order to obtain optimal enantioseparations. Under the optimized conditions, the capillaries exhibited excellent chiral recognition ability for six Dns-amino acids (the DL-forms of alanine, leucine, lsoleucine, valine, methionine, glutamic acid) and provided a promising way for the preparation of chiral column.
KeywordsCEC Magnetic nanoparticles Open-tubular capillary Enantiomeric separation Dns-amino acids Magnetic coationg Ionic liquid
magnetite nanoparticle coated with β-cyclodextrin
magnetite nanoparticle coated with mono-6-deoxy-6-(1-methylimidazolium)-β -cyclodextrin tosylate nanoparticles
6-MI-β-CD+OTs−, mono-6-deoxy-6-(1-methylimidazolium)-β -cyclodextrin tosylate.
This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.BK20141353) and the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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