Advertisement

Microchimica Acta

, 186:161 | Cite as

Colorimetric enzymatic determination of glucose based on etching of gold nanorods by iodine and using carbon quantum dots as peroxidase mimics

  • Qingmei Zhong
  • Yuye Chen
  • Xiu Qin
  • Yilin WangEmail author
  • Chunling Yuan
  • Yuanjin Xu
Original Paper
  • 20 Downloads

Abstract

Carbon quantum dots (CQDs) with peroxidase-mimicking activity were successfully prepared from litchi rind. A colorimetric method for glucose determination was developed based on etching of gold nanorods (GNRs) using CQDs as peroxidase mimetic. The glucose oxidase-catalyzed oxidation of glucose leads to the generation of H2O2 which oxidizes added iodide under formation of elemental iodine under the catalytic action of CQDs. Iodine then etches the GNRs along the longitudinal direction due to the higher reaction activities at the tips of GNRs. This results in a stepwise decrease in the maximum absorption wavelength of the GNRs, from initially 953 nm to finally 645 nm. Under the optimized conditions, the shift in the maximum absorption wavelength decreases linearly in the 0.01–2.0 mM glucose concentration range, and the detection limit is 3.0 μM. Importantly, this method was applied to the determination of glucose in human serum. It is perceived that the CQDs are valuable peroxidase mimics due to their ease of preparation, low costs and stable catalytic activity.

Graphical abstract

Carbon quantum dots were prepared from litchi rind. They can induce the oxidation of gold nanorods in the presence of I ions and H2O2. This finding was applied to design a colorimetric assay for glucose.

Keywords

Spectrophotometry Optical assay Nanomaterial Peroxidase mimetic Glucose oxidase Enzyme mimicking Blood plasma Glucose detection 

Notes

Acknowledgements

The present work was supported by the Natural Science Foundation of China (21567002) and the Opening Project of Guangxi Key Laboratory of Bio Refinery (GXKLB-201801).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3291_MOESM1_ESM.doc (3.4 mb)
ESM 1 (DOC 3488 kb)

References

  1. 1.
    Yu J, Ge L, Huang J, Wang S, Ge S (2011) Microfluidic paper-based chemiluminescence biosensor for simultaneous determination of glucose and uric acid. Lab Chip 11:1286–1291.  https://doi.org/10.1039/C0LC00524J CrossRefPubMedGoogle Scholar
  2. 2.
    van Enter BJ, von Hauff E (2018) Challenges and perspectives in continuous glucose monitoring. Chem Commun 54:5032–5045.  https://doi.org/10.1039/c8cc01678j CrossRefGoogle Scholar
  3. 3.
    Wang HC, Lee AR (2015) Recent developments in blood glucose sensors. J Food Drug Anal 23:191–200.  https://doi.org/10.1016/j.jfda.2014.12.001 CrossRefPubMedGoogle Scholar
  4. 4.
    Su L, Yang L, Sun Q, Zhao T, Liu B, Jiang C, Zhang Z (2018) A ratiometric fluorescent paper sensor for consecutive color change-based visual determination of blood glucose in serum. New J Chem 42:6867–6872.  https://doi.org/10.1039/C8NJ00502H CrossRefGoogle Scholar
  5. 5.
    Ding L, Gong Z, Yan M, Yu J, Song X (2017) Determination of glucose by using fluorescent silicon nanoparticles and an inner filter caused by peroxidase-induced oxidation of o-phenylenediamine by hydrogen peroxide. Microchim Acta 184:4531–4536.  https://doi.org/10.1007/s00604-017-2445-3 CrossRefGoogle Scholar
  6. 6.
    Mutuyimana FP, Liu J, Na M, Nsanzamahoro S, Rao Z, Chen H, Chen X (2018) Synthesis of orange-red emissive carbon dots for fluorometric enzymatic determination of glucose. Microchim Acta 185:518–518.  https://doi.org/10.1007/s00604-018-3041-x CrossRefGoogle Scholar
  7. 7.
    Cai T, Gao Y, Yan J, Wu Y, Di J (2017) Visual detection of glucose using triangular silver nanoplates and gold nanoparticles. RSC Adv 7:29122–29128.  https://doi.org/10.1039/c7ra00593h CrossRefGoogle Scholar
  8. 8.
    Samanta A, Dhar BB, Devi RN (2012) Novel porous silica encapsulated Au nanoreactors as peroxidase mimic for one-pot glucose detection. New J Chem 36:2625–2629.  https://doi.org/10.1039/c2nj40665a CrossRefGoogle Scholar
  9. 9.
    Fan Y, Huang Y (2012) The effective peroxidase-like activity of chitosan-functionalized CoFe2O4 nanoparticles for chemiluminescence sensing of hydrogen peroxide and glucose. Analyst 137:1225–1231.  https://doi.org/10.1039/c2an16105b CrossRefPubMedGoogle Scholar
  10. 10.
    Hallaj T, Amjadi M, Song Z, Bagheri R (2018) Strong enhancement of the chemiluminescence of the Cu(II)-H2O2 system on addition of carbon nitride quantum dots, and its application to the detection of H2O2 and glucose. Microchim Acta 185:67.  https://doi.org/10.1007/s00604-017-2547-y CrossRefGoogle Scholar
  11. 11.
    Kitte SA, Gao W, Zholudov YT, Qi L, Nsabimana A, Liu Z, Xu G (2017) Stainless steel electrode for sensitive Luminol Electrochemiluminescent detection of H2O2, glucose, and glucose oxidase activity. Anal Chem 89:9864–9869.  https://doi.org/10.1021/acs.analchem.7b01939 CrossRefPubMedGoogle Scholar
  12. 12.
    Luo F, Lin Y, Zheng L, Lin X, Chi Y (2015) Encapsulation of hemin in metal organic frameworks for catalyzing the Chemiluminescence reaction of the H2O2-Luminol system and detecting glucose in the neutral condition. ACS Appl Mater Interfaces 7:11322–11329.  https://doi.org/10.1021/acsami.5b01706 CrossRefPubMedGoogle Scholar
  13. 13.
    Liao H, Liu G, Liu Y, Li R, Fu W, Hu L (2017) Aggregation-induced accelerating peroxidase-like activity of gold nanoclusters and their applications for colorimetric Pb2+ detection. Chem Commun 53:10160–10163.  https://doi.org/10.1039/C7CC05409B CrossRefGoogle Scholar
  14. 14.
    Liao H, Hu L, Zhang Y, Yu X, Liu Y, Li R (2018) A highly selective colorimetric sulfide assay based on the inhibition of the peroxidase-like activity of copper nanoclusters. Microchim Acta 185:143.  https://doi.org/10.1007/s00604-018-2701-1 CrossRefGoogle Scholar
  15. 15.
    Nasir M, Nawaz MH, Latif U, Yaqub M, Hayat A, Rahim A (2017) An overview on enzyme-mimicking nanomaterials for use in electrochemical and optical assays. Microchim Acta 184:323–342.  https://doi.org/10.1007/s00604-016-2036-8 CrossRefGoogle Scholar
  16. 16.
    Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583.  https://doi.org/10.1038/nnano.2007.260 CrossRefPubMedGoogle Scholar
  17. 17.
    Mu J, Wang Y, Zhao M, Zhang L (2012) Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun 48:2540–2542.  https://doi.org/10.1039/C2CC17013B CrossRefGoogle Scholar
  18. 18.
    Wang Y, Zhang D, Wang J (2018) Metastable alpha-AgVO3 microrods as peroxidase mimetics for colorimetric determination of H2O2. Microchim Acta 185(1).  https://doi.org/10.1007/s00604-017-2562-z
  19. 19.
    Huang L, Zhu W, Zhang W, Chen K, Wang J, Wang R, Yang Q, Hu N, Suo Y, Wang J (2018) Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose. Microchim Acta 185:7.  https://doi.org/10.1007/s00604-017-2552-1 CrossRefGoogle Scholar
  20. 20.
    Ding C, Yan Y, Xiang D, Zhang C, Xian Y (2016) Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay. Microchim Acta 183:625–631.  https://doi.org/10.1007/s00604-015-1690-6 CrossRefGoogle Scholar
  21. 21.
    Choleva TG, Gatselou VA, Tsogas GZ, Giokas DL (2018) Intrinsic peroxidase-like activity of rhodium nanoparticles, and their application to the colorimetric determination of hydrogen peroxide and glucose. Microchim Acta 185:22.  https://doi.org/10.1007/s00604-017-2582-8 CrossRefGoogle Scholar
  22. 22.
    Xia Y, Ye J, Tan K, Wang J, Yang G (2013) Colorimetric visualization of glucose at the submicromole level in serum by a homogenous silver Nanoprism–glucose oxidase system. Anal Chem 85:6241–6247.  https://doi.org/10.1021/ac303591n CrossRefPubMedGoogle Scholar
  23. 23.
    Rastogi L, Karunasagar D, Sashidhar RB, Giri A (2017) Peroxidase-like activity of gum kondagogu reduced/stabilized palladium nanoparticles and its analytical application for colorimetric detection of glucose in biological samples. Sensors Actuators B Chem 240:1182–1188.  https://doi.org/10.1016/j.snb.2016.09.066 CrossRefGoogle Scholar
  24. 24.
    Jv Y, Li B, Cao R (2010) Positively-charged gold nanoparticles as peroxidiase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun 46:8017–8019.  https://doi.org/10.1039/C0CC02698K CrossRefGoogle Scholar
  25. 25.
    Wang Y, Jiang K, Zhu J, Zhang L, Lin H (2015) A FRET-based carbon dot-MnO2 nanosheet architecture for glutathione sensing in human whole blood samples. Chem Commun 51:12748–12751.  https://doi.org/10.1039/c5cc04905a CrossRefGoogle Scholar
  26. 26.
    Hsu PC, Chang HT (2012) Synthesis of high-quality carbon nanodots from hydrophilic compounds: role of functional groups. Chem Commun 48:3984–3986.  https://doi.org/10.1039/c2cc30188a CrossRefGoogle Scholar
  27. 27.
    Mao X-J, Zheng H-Z, Long Y-J, Du J, Hao J-Y, Wang L-L, Zhou D-B (2010) Study on the fluorescence characteristics of carbon dots. Spectrochim Acta A Mol Biomol Spectrosc 75:553–557.  https://doi.org/10.1016/j.saa.2009.11.015 CrossRefPubMedGoogle Scholar
  28. 28.
    Dehghani A, Ardekani SM, Hassan M, Gomes VG (2018) Collagen derived carbon quantum dots for cell imaging in 3D scaffolds via two-photon spectroscopy. Carbon 131:238–245.  https://doi.org/10.1016/j.carbon.2018.02.006 CrossRefGoogle Scholar
  29. 29.
    Martindale BCM, Hutton GAM, Caputo CA, Reisner E (2015) Solar hydrogen production using carbon quantum dots and a molecular nickel catalyst. J Am Chem Soc 137:6018–6025.  https://doi.org/10.1021/jacs.5b01650 CrossRefPubMedGoogle Scholar
  30. 30.
    Li H, Liu R, Kong W, Liu J, Liu Y, Zhou L, Zhang X, Lee S-T, Kang Z (2014) Carbon quantum dots with photo-generated proton property as efficient visible light controlled acid catalyst. Nanoscale 6:867–873.  https://doi.org/10.1039/c3nr03996j CrossRefPubMedGoogle Scholar
  31. 31.
    Shi W, Wang Q, Long Y, Cheng Z, Chen S, Zheng H, Huang Y (2011) Carbon nanodots as peroxidase mimetics and their applications to glucose detection. Chem Commun 47:6695–6697.  https://doi.org/10.1039/c1cc11943e CrossRefGoogle Scholar
  32. 32.
    Wang B, Liu F, Wu Y, Chen Y, Weng B, Li CM (2018) Synthesis of catalytically active multielement-doped carbon dots and application for colorimetric detection of glucose. Sensors Actuators B Chem 255:2601–2607.  https://doi.org/10.1016/j.snb.2017.09.067 CrossRefGoogle Scholar
  33. 33.
    Lin L, Song X, Chen Y, Rong M, Zhao T, Wang Y, Jiang Y, Chen X (2015) Intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots and their application in the colorimetric detection of H2O2 and glucose. Anal Chim Acta 869:89–95.  https://doi.org/10.1016/j.aca.2015.02.024 CrossRefPubMedGoogle Scholar
  34. 34.
    Zhang Z, Chen Z, Cheng F, Zhang Y, Chen L (2017) Highly sensitive on-site detection of glucose in human urine with naked eye based on enzymatic-like reaction mediated etching of gold nanorods. Biosens Bioelectron 89:932–936.  https://doi.org/10.1016/j.bios.2016.09.090 CrossRefPubMedGoogle Scholar
  35. 35.
    Shi X, Lu D, Wang Z, Zhang D, Gao W, Zhang C, Deng J, Guo S (2018) Colorimetric and visual determination of acrylamide via acrylamide-mediated polymerization of acrylamide-functionalized gold nanoparticles. Microchim Acta 185:522.  https://doi.org/10.1007/s00604-018-3062-5 CrossRefGoogle Scholar
  36. 36.
    Saa L, Coronado-Puchau M, Pavlov V, Liz-Marzan LM (2014) Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection. Nanoscale 6:7405–7409.  https://doi.org/10.1039/c4nr01323a CrossRefPubMedGoogle Scholar
  37. 37.
    Liu X, Zhang S, Tan P, Zhou J, Huang Y, Nie Z, Yao S (2013) A plasmonic blood glucose monitor based on enzymatic etching of gold nanorods. Chem Commun 49:1856–1858.  https://doi.org/10.1039/c3cc38476d CrossRefGoogle Scholar
  38. 38.
    Zhong Q, Chen Y, Su A, Wang Y (2018) Synthesis of catalytically active carbon quantum dots and its application for colorimetric detection of glutathione. Sensors Actuators B Chem 273:1098–1102.  https://doi.org/10.1016/j.snb.2018.07.026 CrossRefGoogle Scholar
  39. 39.
    Cheng C, Shi Y, Li M, Xing M, Wu Q (2017) Carbon quantum dots from carbonized walnut shells: structural evolution, fluorescence characteristics, and intracellular bioimaging. Mater Sci Eng C 79:473–480.  https://doi.org/10.1016/j.msec.2017.05.094 CrossRefGoogle Scholar
  40. 40.
    Zhang Y, Gao Z, Zhang W, Wang W, Chang J, Kai J (2018) Fluorescent carbon dots as nano probe for determination of lidocaine hydrochloride. Sensors Actuators B Chem 262:928–937.  https://doi.org/10.1016/j.snb.2018.02.079 CrossRefGoogle Scholar
  41. 41.
    Sahoo NK, Das S, Jana GC, Aktara MN, Patra A, Maji A, Beg M, Jha PK, Hossain M (2019) Eco-friendly synthesis of a highly fluorescent carbon dots from spider silk and its application towards Hg (II) ions detection in real sample and living cells. Microchem J 144:479–488.  https://doi.org/10.1016/j.microc.2018.10.006 CrossRefGoogle Scholar
  42. 42.
    Song Y, Qu K, Zhao C, Ren J, Qu X (2010) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22:2206–2210.  https://doi.org/10.1002/adma.200903783 CrossRefPubMedGoogle Scholar
  43. 43.
    Zhu Q, Wu J, Zhao J, Ni W (2015) Role of bromide in hydrogen peroxide oxidation of CTAB-stabilized gold Nanorods in aqueous solutions. Langmuir 31:4072–4077.  https://doi.org/10.1021/acs.langmuir.5b00137 CrossRefPubMedGoogle Scholar
  44. 44.
    Xu D, Mao J, He Y, Yeung ES (2014) Size-tunable synthesis of high-quality gold nanorods under basic conditions by using H2O2 as the reducing agent. J Mater Chem C 2:4989–4996.  https://doi.org/10.1039/C4TC00483C CrossRefGoogle Scholar
  45. 45.
    Tsung CK, Kou XS, Shi QH, Zhang JP, Yeung MH, Wang JF, Stucky GD (2006) Selective shortening of single-crystalline gold nanorods by mild oxidation. J Am Chem Soc 128:5352–5353.  https://doi.org/10.1021/ja060447t CrossRefPubMedGoogle Scholar
  46. 46.
    Yang H, Yang R, Zhang P, Qin Y, Chen T, Ye F (2017) A bimetallic (Co/2Fe) metal-organic framework with oxidase and peroxidase mimicking activity for colorimetric detection of hydrogen peroxide. Microchim Acta 184:4629–4635.  https://doi.org/10.1007/s00604-017-2509-4 CrossRefGoogle Scholar
  47. 47.
    Shen S, Jia M, Tang Z, Chang S, Shi P, Yang J (2018) Preparation and application of Mn-doped Zn0.5Cd0.5S @ ZnS nanorods with high quantum yield as sensitive fluorescence probe for detection of glucose. Mater Res Bull 106:471–477.  https://doi.org/10.1016/j.materresbull.2018.06.043 CrossRefGoogle Scholar
  48. 48.
    Karim MN, Anderson SR, Singh S, Ramanathan R, Bansal V (2018) Nanostructured silver fabric as a free-standing NanoZyme for colorimetric detection of glucose in urine. Biosens Bioelectron 110:8–15.  https://doi.org/10.1016/j.bios.2018.03.025 CrossRefPubMedGoogle Scholar
  49. 49.
    Shin HY, Kim B-G, Cho S, Lee J, Na HB, Kim MI (2017) Visual determination of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of magnetic nanoparticles functionalized with a poly(ethylene glycol) derivative. Microchim Acta 184:2115–2122.  https://doi.org/10.1007/s00604-017-2198-z CrossRefGoogle Scholar
  50. 50.
    Chen J, Chen Q, Chen J, Qiu H (2016) Magnetic carbon nitride nanocomposites as enhanced peroxidase mimetics for use in colorimetric bioassays, and their application to the determination of H2O2 and glucose. Microchim Acta 183:3191–3199.  https://doi.org/10.1007/s00604-016-1972-7 CrossRefGoogle Scholar
  51. 51.
    Tran HV, Nguyen TV, Nguyen ND, Piro B, Huynh CD (2018) A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine. Microchim Acta 185:270.  https://doi.org/10.1007/s00604-018-2804-8 CrossRefGoogle Scholar
  52. 52.
    Lin Y, Zhao M, Guo Y, Ma X, Luo F, Guo L, Qiu B, Chen G, Lin Z (2016) Multicolor Colormetric biosensor for the determination of glucose based on the etching of gold Nanorods. Sci Rep 6:1–7.  https://doi.org/10.1038/srep37879 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Qingmei Zhong
    • 1
  • Yuye Chen
    • 1
  • Xiu Qin
    • 1
  • Yilin Wang
    • 1
    Email author
  • Chunling Yuan
    • 1
  • Yuanjin Xu
    • 1
  1. 1.School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of BiorefineryGuangxi UniversityNanningChina

Personalised recommendations