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Microchimica Acta

, 186:157 | Cite as

Multicolor emitting N/S-doped carbon dots as a fluorescent probe for imaging pathogenic bacteria and human buccal epithelial cells

  • Abhishek Pathak
  • Suneesh PV
  • John Stanley
  • T. G. Satheesh BabuEmail author
Original Paper
  • 38 Downloads

Abstract

Carbon dots co-doped with nitrogen and sulfur (NSCDs) were obtained from thiourea and TAE (Tris-acetate-ethylenediamine) buffer using microwave assisted hydrothermal synthesis. The synergistic presence of nitrogen and sulfur as a dopant results in teasing fluorescence properties and a fluorescence quantum yield of 57%. An HR-TEM study showed the NSCDs to be mono-dispersed and seemingly spherical with an average hydrodynamic diameter of 3.6 ± 0.88 nm. The NSCDs are nontoxic as proven by an MTT assay for cytotoxicity. The optical characterization was done by using UV-Vis absorption and fluorescence spectroscopy which revealed excitation wavelength-dependent multicolor emissions. The characterization of surface topology was done by using X-ray diffraction, FTIR, and X-ray photoelectron spectroscopy. The NSCDs were used to image various pathogenic bacteria (E. coli, Klebsiella, Pseudomonas & Staphylococcus) and human buccal epithelial cells by applying multicolor fluorometry.

Graphical abstract

Schematic presentation of microwave-assisted hydrothermal synthesis of nitrogen and sulfur doped carbon dots (NSCD) based on Thiourea and 50X Tris-acetate-ethylenediamine (TAE) buffer having multicolor fluorescence, used for tagging and imaging pathogenic bacteria and Human buccal epithelial cells using fluorescence microscope.

Keywords

Thiourea TAE buffer Doping Microwave-assisted hydrothermal synthesis Tagging Fluorescence microscopy Fluorescence quantum yield Multicolor fluorometry 

Notes

Acknowledgements

The authors gratefully acknowledge the Department of Biotechnology (DBT), Govt. of India for the financial support through the project no. 102SAN/2237/2016-2017 and project no. 102/IFD/SAN/1409/2018-2019. The authors would like to thank Sophisticated Analytical Instruments Facility (SAIF), Cochin University of Science and Technology (CUSAT) for their unsparing support in HR-TEM image acquisition. The authors would also like to thank Mr. Jayesh Vasudeva Adhikari (University of Southern California), Mr. Prateek Katare (Indian Institute of Science, Bangalore) and Ms. Divya Nair (Amrita School of Biotechnology, Kollam) for their generous help in providing the presentable schematics, MATLAB codes for particle size estimation and several other characterization techniques.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3270_MOESM1_ESM.docx (12.6 mb)
ESM 1 (DOCX 12935 kb)

References

  1. 1.
    Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK (2018) Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol 9:1050–1074CrossRefGoogle Scholar
  2. 2.
    DaCosta MV, Doughan S, Han Y, Krull UJ (2014) Lanthanide upconversion nanoparticles and applications in bioassays and bioimaging: a review. Anal Chim Acta 832:1–33CrossRefGoogle Scholar
  3. 3.
    Baetke SC, Lammers T, Kiessling F (2015) Applications of nanoparticles for diagnosis and therapy of cancer. Br J Radiol 88:20150207CrossRefGoogle Scholar
  4. 4.
    De Jong WH, Borm PJ (2008) Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine 3:133–149CrossRefGoogle Scholar
  5. 5.
    Wang L, Zhao W, Tan W (2008) Bioconjugated silica nanoparticles: development and applications. Nano Res 1:99–115CrossRefGoogle Scholar
  6. 6.
    Wolfbeis OS (2015) An overview of nanoparticles commonly used in fluorescent bioimaging. Chem Soc Rev 44:4743–4768CrossRefGoogle Scholar
  7. 7.
    Nune SK, Gunda P, Thallapally PK, Lin Y-Y, Forrest ML, Berkland CJ (2009) Nanoparticles for biomedical imaging. Expert Opin Drug Deliv 6:1175–1194CrossRefGoogle Scholar
  8. 8.
    Bannunah AM, Vllasaliu D, Lord J, Stolnik S (2014) Mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge. Mol Pharm 11:4363–4373CrossRefGoogle Scholar
  9. 9.
    Lee S-C, Kim M-S, Yoo K-C, Ha N-R, Moon J-Y, Lee S-J, Yoon MY (2017) Sensitive fluorescent imaging of salmonella enteritidis and salmonella typhimurium using a polyvalent directed peptide polymer. Microchim Acta 184:2611–2620CrossRefGoogle Scholar
  10. 10.
    El-Ansary A, Faddah LM (2010) Nanoparticles as biochemical sensors. Nanotechnol Sci Appl 3:65–76CrossRefGoogle Scholar
  11. 11.
    Velusamy N, Binoy A, Bobba KN, Nedungadi D, Mishra N, Bhuniya S (2017) A bioorthogonal fluorescent probe for mitochondrial hydrogen sulfide: new strategy for cancer cell labeling. J Chem Soc Chem Commun 53:8802–8805CrossRefGoogle Scholar
  12. 12.
    Zhang J, Yu S-H (2016) Carbon dots: large-scale synthesis, sensing and bioimaging. Mater Today 19:382–393CrossRefGoogle Scholar
  13. 13.
    Li J, Jiao Y, Feng L, Zhong Y, Zuo G, Xie A, Dong W (2017) Highly n,p-doped carbon dots: rational design, photoluminescence and cellular imaging. Microchim Acta 184:2933–2940CrossRefGoogle Scholar
  14. 14.
    Zheng M, Ruan S, Liu S, Sun T, Qu D, Zhao H, Xie Z, Gao H, Jing X, Sun Z (2015) Self-targeting fluorescent carbon dots for diagnosis of brain Cancer cells. ACS Nano 9:11455–11461CrossRefGoogle Scholar
  15. 15.
    Wang D, Wang Z, Zhan Q, Pu Y, Wang J-X, Foster NR, Dai L (2017) Facile and scalable preparation of fluorescent carbon dots for multifunctional applications. Engineering 3:402–408CrossRefGoogle Scholar
  16. 16.
    Qu K, Wang J, Ren J, Qu X (2013) Carbon dots prepared by hydrothermal treatment of dopamine as an effective fluorescent sensing platform for the label-free detection of iron (iii) ions and dopamine. Chem Eur J 19:7243–7249CrossRefGoogle Scholar
  17. 17.
    Wang Q, Liu X, Zhang L, Lv Y (2012) Microwave-assisted synthesis of carbon nanodots through an eggshell membrane and their fluorescent application. Analyst 137:5392–5397CrossRefGoogle Scholar
  18. 18.
    Zhou J, Sheng Z, Han H, Zou M, Li C (2012) Facile synthesis of fluorescent carbon dots using watermelon peel as a carbon source. Mater Lett 66:222–224CrossRefGoogle Scholar
  19. 19.
    Sahu S, Behera B, Maiti TK, Mohapatra S (2012) Simple one-step synthesis of highly luminescent carbon dots from orange juice: application as excellent bio-imaging agents. J Chem Soc Chem Commun 48:8835–8837CrossRefGoogle Scholar
  20. 20.
    Das P, Bose M, Ganguly S, Mondal S, Das AK, Banerjee S, Das NC (2017) Green approach to photoluminescent carbon dots for imaging of gram-negative bacteria Escherichia coli. Nanotechnology 28:195501CrossRefGoogle Scholar
  21. 21.
    Xu H, Yang X, Li G, Zhao C, Liao X (2015) Green synthesis of fluorescent carbon dots for selective detection of Tartrazine in food samples. J Agric Food Chem 63:6707–6714CrossRefGoogle Scholar
  22. 22.
    Kalytchuk S, Polkov K, Wang Y, Froning JP, Cepe K, Rogach AL, Zboil R (2017) Carbon dot Nanothermometry: intracellular photoluminescence lifetime thermal sensing. ACS Nano 11:1432–1442CrossRefGoogle Scholar
  23. 23.
    Cui X, Wang Y, Liu J, Yang Q, Zhang B, Gao Y, Wang Y, Lu G (2017) Dual functional N- and S-co-doped carbon dots as the sensor for temperature and Fe3+ ions. Sens Actuator B-Chem 242:1272–1280CrossRefGoogle Scholar
  24. 24.
    Shen C, Wang J, Cao Y, Lu Y (2015) Facile access to B-doped solid-state fluorescent carbon dots toward light emitting devices and cell imaging agents. J Mater Chem C 3:6668–6675CrossRefGoogle Scholar
  25. 25.
    Bao R, Chen Z, Zhao Z, Sun X, Zhang J, Hou L, Yuan C (2018) Green and facile synthesis of nitrogen and phosphorus co-doped carbon quantum dots towards fluorescent ink and sensing applications. Nanomaterials 8:386CrossRefGoogle Scholar
  26. 26.
    Xu Y, Li D, Liu M, Niu F, Liu J, Wang E (2017) Enhanced-quantum yield sulfur/nitrogen co-doped fluorescent carbon nanodots produced from biomass Enteromorpha prolifera: synthesis, posttreatment, applications and mechanism study. Sci Rep 7:4499CrossRefGoogle Scholar
  27. 27.
    Sun Y, Shen C, Wang J, Lu Y (2015) Facile synthesis of biocompatible n, s-doped carbon dots for cell imaging and ion detecting. RSC Adv 5:16368–16375CrossRefGoogle Scholar
  28. 28.
    Bhushan B, Kumar SU, Gopinath P (2016) Multifunctional carbon dots as effcient fluorescent nanotags for tracking cells through successive generations. J Mater Chem B 4:4862–4871CrossRefGoogle Scholar
  29. 29.
    Zhai X, Zhang P, Liu C, Bai T, Li W, Dai L, Liu W (2012) Highly luminescent carbon nanodots by microwave-assisted pyrolysis. J Chem Soc Chem Commun 48:7955–7957CrossRefGoogle Scholar
  30. 30.
    Meiling TT, Cywiski PJ, Bald I (2016) White carbon: fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis. Sci Rep 6:28557CrossRefGoogle Scholar
  31. 31.
    Li Z, Lu C, Xia Z, Zhou Y, Luo Z (2007) X-ray diffraction patterns of graphite and turbostratic carbon. Carbon 45:1686–1695CrossRefGoogle Scholar
  32. 32.
    Rajkumari J, Singha LP, Pandey P (2018) Genomic insights of aromatic hydrocarbon degrading klebsiella pneumoniae awd5 with plant growth promoting attributes: a paradigm of soil isolate with elements of biodegradation. 3 Biotech 8:118CrossRefGoogle Scholar
  33. 33.
    Shen J, Zhang T, Cai Y, Chen X, Shang S, Li J (2017) Highly fluorescent N,S-co-doped carbon dots: synthesis and multiple applications. New J Chem 41:11125–11137CrossRefGoogle Scholar
  34. 34.
    Chen J, Liu J, Li J, Xu L, Qiao Y (2017) One-pot synthesis of nitrogen and sulfur co-doped carbon dots and its application for sensor and multicolor cellular imaging. J Colloid Interface Sci 485:167–174CrossRefGoogle Scholar
  35. 35.
    Chai L, Zhou J, Feng H, Tang C, Huang Y, Qian Z (2015) Functionalized carbon quantum dots with dopamine for Tyrosinase activity monitoring and inhibitor screening: in vitro and intracellular investigation. ACS Appl Mater Interfaces 7:23564–23574CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Abhishek Pathak
    • 1
  • Suneesh PV
    • 1
    • 2
  • John Stanley
    • 1
    • 2
  • T. G. Satheesh Babu
    • 1
    • 2
    Email author
  1. 1.Amrita Biosensor Research Lab, Amrita School of EngineeringAmrita Vishwa VidyapeethamCoimbatoreIndia
  2. 2.Department of Sciences, Amrita School of EngineeringAmrita Vishwa VidyapeethamCoimbatoreIndia

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