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Microchimica Acta

, 186:149 | Cite as

A DNA functionalized porphyrinic metal-organic framework as a peroxidase mimicking catalyst for amperometric determination of the activity of T4 polynucleotide kinase

  • Weiling Song
  • Wenshuo Yin
  • Zhonghui Zhang
  • Peng He
  • Xiaoyan Yang
  • Xiaoru ZhangEmail author
Original Paper
  • 18 Downloads

Abstract

An electrochemical method is described for the sensitive detection of the activity of the enzyme T4 polynucleotide kinase (PNK) by using a DNA functionalized porphyrinic metal-organic framework (L/(Fe-P)n-MOF). In the presence of PNK, the hairpin oligonucleotide (HP1) becomes phosphorylated, and the trigger is released by lambda exonuclease (λ exo). The trigger DNA hybridizes with hairpin probe (immobilized on the gold electrode) to form a nicking endonuclease cleavage site. Thus, a single-strand capture probe is employed to hybridize with L/(Fe-P)n-MOF. The (Fe-P)n-MOF is a peroxidase mimicking material with high catalytic efficiency. By using this amplification strategy, an electrochemical signal is procured that allows for the determination of T4 PNK in the 1.0 mU·mL−1 to 1.0 U·mL−1 with a detection limit of 0.62 mU·mL−1. The method is selective and can be used to screen for enzyme inhibitors. Conceivably, the (Fe-P)n-MOF can also be used to detect other analytes via its peroxidase-mimicking activity.

Graphical abstract

Schematic presentation of T4 polynucleotide kinase (PNK) detection. Two hairpin DNAs (HP) and a porphyrinic metal-organic framework with peroxidase-mimicking activity are used. The detection limit is 0.62 mU mL−1 with enzyme assisted signal amplification. This method is selective and can be used to screen for enzyme inhibitors.

Keywords

MOF Peroxidase mimetic Electrochemical assay DNA enzyme Lambda exonuclease Tetramethylbenzidine H2O2 Gold nanoparticles 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21505082, 21705086, 21775080), a project of Shandong Province Higher Educational Science and Technology Program (Grant J16LC10), the Key Research and Development project of Shandong Province (No. 2017GSF221004, No. 2018GGX102001).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3269_MOESM1_ESM.docx (975 kb)
ESM 1 (DOCX 975 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Weiling Song
    • 1
  • Wenshuo Yin
    • 1
  • Zhonghui Zhang
    • 1
  • Peng He
    • 1
  • Xiaoyan Yang
    • 1
  • Xiaoru Zhang
    • 1
    Email author
  1. 1.Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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