Microchimica Acta

, 186:138 | Cite as

Simultaneous colorimetric determination of acute myocardial infarction biomarkers by integrating self-assembled 3D gold nanovesicles into a multiple immunosorbent assay

  • Qinli Pu
  • Xuanhua Yang
  • Yongcan Guo
  • Tao Dai
  • Tingyan Yang
  • Xinying Ou
  • Junlong Li
  • Shangchun Sheng
  • Guoming XieEmail author
Original Paper


An improved enzyme-free immunosorbent assay is described for the simultaneous detection of the myocardial infarction biomarkers N-terminal pro B type natriuretic peptide (NT-proBNP), creatine kinase-MB (CK-MB), and cardiac muscle troponin T (cTnT). The assay integrates 3D gold nanovesicles (GNVs) and three allochroic agents (phenolphthalein, methyl red, bromothymol blue). The pH regulated allochroic agents were enwrapped in GNVs to acts as ultrasensitive nanoprobes. Loading can be controlled by adjusting the temperature to efficiently load and release the allochroic agents. This bare-eye multicolor assay has limits of detection of 70 pg·mL−1 for NT-proBNP, 910 pg·mL−1 for CK-MB, and 7.8 pg·mL−1 for cTnT. Other features include (a) a linear range that extends over a wide range and sometimes is better than conventional HRP-based immunoassays, and (b) a precision that is comparable to immunofluorescence assays as used in the clinical laboratory.

Graphical abstract

Schematic presentation of an improved enzyme-free immunosorbent assay (EFISA). It integrates 3D gold nano-vesicles (GNVs) and allochroic agents for the simultaneous detection of acute myocardial infarction (AMI) biomarkers (N-terminal prohormone of brain natriuretic peptide (NT-proBNP), kinase-muscle/brain test (CK-MB), and cardiac muscle troponin (cTnT)).


Enzyme-free immunosorbent assay Gold nanovesicles Nanoprobes Allochroic agents Multiplex detection N-terminal pro B type natriuretic peptide Creatine kinase-MB Cardiac muscle troponin T Plasma samples 



This research work was financially supported by the National Natural Science Foundation of China (No. 81672112, 81702101), Chongqing Technology Innovation and Application Demonstration Project (cstc2018jscx-msybX0010) and Key Project of Education Department of Sichuan(No. 16ZA0181).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3242_MOESM1_ESM.doc (1.6 mb)
ESM 1 (DOC 1682 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Qinli Pu
    • 1
  • Xuanhua Yang
    • 2
  • Yongcan Guo
    • 3
  • Tao Dai
    • 1
  • Tingyan Yang
    • 1
  • Xinying Ou
    • 1
  • Junlong Li
    • 4
  • Shangchun Sheng
    • 5
  • Guoming Xie
    • 1
    Email author
  1. 1.Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory MedicineChongqing Medical UniversityChongqingPeople’s Republic of China
  2. 2.Department of Gastrointestinal Surgery, Second Clinical Medical Institution of North Sichuan Medical CollegeNanchong Central HospitalNanchongPeople’s Republic of China
  3. 3.Clinical Laboratory of Traditional Chinese Medicine Hospital Affiliated to Southwest Medical UniversityLuzhouPeople’s Republic of China
  4. 4.University-Town Hospital of Chongqing Medical UniversityChongqingPeople’s Republic of China
  5. 5.Clinical Laboratory of Hospital Affiliated to Chengdu UniversitySichuanPeople’s Republic of China

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