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Microchimica Acta

, 186:93 | Cite as

Simultaneous fluorometric determination of the DNAs of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus by using an ultrathin metal-organic framework (type Cu-TCPP)

  • Qiming Qiu
  • Huayun Chen
  • Shengna Ying
  • Sumaira Sharif
  • Zhiheng You
  • Yixian WangEmail author
  • Yibin Ying
Original Paper
  • 32 Downloads

Abstract

Ultrathin (<10 nm) nanosheets of a metal-organic framework (MOF-NSs) were prepared in high-yield and scalable production by a surfactant-assisted one-step method. The MOF-NSs possess distinguished affinity for ssDNA but not for dsDNA. This causes the fluorescence of the labeled DNA to be quenched. On binding to the target DNA (shown here for Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus), the labeled duplex is released and the fluorescence of the label is restored. The labels Texas Red, Cy3 and FAM were used and give red, red or green fluorescence depending on the kind of pathogen. The detection limits are 28 pM, 35 pM and 15 pM for the gene segments of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus, respectively.

Graphical abstract

Schematic of an ultrasensitive fluorescent biosensor for multiplex detection of pathogenic DNAs based on ultrathin MOF nanosheets (type Cu-TCPP).

Keywords

Two dimensional nanomaterials Surfactant-assisted synthesis FRET Pathogens Fluorescence sensor Multiplex detection 

Notes

Acknowledgements

The authors are grateful for financial support from National Key Research and Development Program of China (No. 2017YFC1601700) and National Natural Science Foundation of China (No. 31401568).

Compliance with ethical standards

The author declare that they have no competing interests.

Supplementary material

604_2019_3226_MOESM1_ESM.pdf (664 kb)
ESM 1 (PDF 663 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Zhejiang A&F UniversityHangzhouPeople’s Republic of China

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