Advertisement

Microchimica Acta

, 186:123 | Cite as

Colorimetric determination of ascorbic acid and the activity of alkaline phosphatase based on the inhibition of the peroxidase-like activity of citric acid-capped Prussian Blue nanocubes

  • Tengteng Wu
  • Wenli Hou
  • Zhangyan Ma
  • Meiling LiuEmail author
  • Xiaoying LiuEmail author
  • Youyu Zhang
  • Shouzhuo Yao
Original Paper
  • 106 Downloads

Abstract

Colorimetric methods are described for the determination of ascorbic acid (AA) and alkaline phosphatase (ALP). Both assays are based on the inhibition of the peroxidase (POx)-like activity of Prussian Blue nanocubes (PB NCs) capped with citric acid. They catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to produce a blue color with an absorption maximum at 652 nm. On addition of AA, the PB NCs are reduced to Prussian White (PW) which does not act as a POx mimic. This results in a decreased rate of the formation of the blue coloration whose intensity decreases with increasing concentration of AA. The assay allows AA to be quantified with a 35 nM detection limit (at 3σ/m). The hydrolysis of AA phosphate by ALP leads to the formation of AA which can be quantified by the above method. Based on this, the activity of ALP can be determined by measurement of the intensity of the blue coloration thus formed. The method can be used to determine the activity of ALP with a detection limit as low as 0.23 U·L−1.

Graphical abstract

Schematic presentation of a method for colorimetric determination of ALP activity. AA obtained by ALP-catalyzed hydrolysis of ascorbic acid phosphate (AAP) inhibits the intrinsic peroxidase-like activity of PB NCs by reducing Prussian Blue nanocrystals (PB NCs) to form inactive Prussian White (PW).

Keywords

Enzyme mimic Nanocubes Prussian White Peroxidase mimetic 3,3,5,5-Tetramethylbenzidine Inhibition Colorimetric assay 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21645008, 21305042, 21375037), Scientific Research Fund of Hunan Provincial Education Department (14B116), Science and Technology Department (14JJ4030), the construct program of the key discipline in Hunan province and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3224_MOESM1_ESM.docx (3.4 mb)
ESM 1 (DOCX 3.36 mb)

References

  1. 1.
    Deng JJ, Yu P, Wang YX, Mao LQ (2015) Real-time ratiometric fluorescent assay for alkaline phosphatase activity with stimulus responsive infinite coordination polymer nanoparticles. Anal Chem 87:3080–3086CrossRefGoogle Scholar
  2. 2.
    Song HW, Wang HY, Li X, Peng YX, Pan JM, Niu XH (2018) Sensitive and selective colorimetric detection of alkaline phosphatase activity based on phosphate anion-quenched oxidase-mimicking activity of Ce(IV) ions. Anal Chim Acta 31:154–161CrossRefGoogle Scholar
  3. 3.
    Takuya H, Masaru S, Kohei T, Hirotaka M, Tomonari U, Hiroki H (2006) Assay of alkaline phosphatase in salmon egg cell cytoplasm with fluorescence detection of enzymatic activity and zinc detection by ICP-MS in relation to metallomics research. B Chem Soc Jpn 79:1211–1214CrossRefGoogle Scholar
  4. 4.
    Ino K, Kanno Y, Arai T, Inoue KY, Takahashi Y, Shiku H, Matsue T (2012) Novel electrochemical methodology for activity estimation of alkaline phosphatase based on solubility difference. Anal Chem 84:7593–7598CrossRefGoogle Scholar
  5. 5.
    Wei H, Chen CG, Han BY, Wang EK (2008) Enzyme colorimetric assay using unmodified silver nanoparticles. Anal Chem 80:7051–7055CrossRefGoogle Scholar
  6. 6.
    Ingram A, Moore BD, Graham D (2009) Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS. Bioorg Med Chem Lett 19:1569–1571CrossRefGoogle Scholar
  7. 7.
    Zhu XH, Zhao TB, Nie Z, Liu Y, Yao SZ (2015) Non-redox modulated fluorescence strategy for sensitive and selective ascorbic acid detection with highly Photoluminescent nitrogen-doped carbon nanoparticles via solid-state synthesis. Anal Chem 87:8524–8530CrossRefGoogle Scholar
  8. 8.
    Qian J, Yang XW, Yang ZT, Zhu GB, Mao HP, Wang K (2015) Multiwalled carbon nanotube@reduced graphene oxide nanoribbon heterostructure: synthesis, intrinsic peroxidase-like catalytic activity, and its application in colorimetric biosensing. J Mater Chem B 3:1624–1632CrossRefGoogle Scholar
  9. 9.
    Song YJ, Chen Y, Feng LY, Ren JS, Qu XG (2011) Selective and quantitative cancer cell detection using target-directed functionalized graphene and its synergetic peroxidase-like activity. Chem Commun 47:4436–4438CrossRefGoogle Scholar
  10. 10.
    Zheng AX, Cong ZX, Wang JR, Li J, Yang HH, Chen GN (2013) Highly-efficient peroxidase-like catalytic activity of graphene dots for biosensing. Biosens Bioelectron 49:519–524CrossRefGoogle Scholar
  11. 11.
    Chang YQ, Zhang Z, Hao JH, Yang WS, Tang JL (2016) A simple label free colorimetric method for glyphosate detection based on the inhibition of peroxidase-like activity of Cu(II). Sensors Actuators B Chem 228:410–415CrossRefGoogle Scholar
  12. 12.
    Dehghani Z, Hosseini M, Mohammadnejad J, Bakhshi B, Rezayan AH (2018) Colorimetric aptasensor for Campylobacter jejuni cells by exploiting the peroxidase like activity of Au@Pd nanoparticles. Microchim Acta 185:448CrossRefGoogle Scholar
  13. 13.
    Wang GL, Jin LY, Dong YM, Wu XM, Li ZJ (2015) Intrinsic enzyme mimicking activity of gold nanoclusters upon visible light triggering and its application for colorimetric trypsin detection. Biosens Bioelectron 64:523–529CrossRefGoogle Scholar
  14. 14.
    Chen W, Chen J, Feng YB, Hong L, Chen QY, Wu LF (2012) Peroxidase-like activity of water-soluble cupric oxide nanoparticles and its analytical application for detection of hydrogen peroxide and glucose. Analyst 137:1706–1712CrossRefGoogle Scholar
  15. 15.
    Wang N, Duan JZ, Shi WJ, Zhai XF, Guan F, Yang LH, Hou BR (2018) A 3-dimensional C/CeO2 hollow nanostructure framework as a peroxidase mimetic, and its application to the colorimetric determination of hydrogen peroxide. Microchim Acta 185:417CrossRefGoogle Scholar
  16. 16.
    Liu HM, Wang BC, Li DH, Zeng XY, Tang X, Gao QS, Cai JY, Cai HH (2018) MoS2 nanosheets with peroxidase mimicking activity as viable dual-mode optical probes for determination and imaging of intracellular hydrogen peroxide. Microchim Acta 185:287CrossRefGoogle Scholar
  17. 17.
    Zhang LL, Han L, Hu P, Wang L, Dong SJ (2013) TiO2 nanotube arrays: intrinsic peroxidase mimetics. Chem Commun 49:10480–10482CrossRefGoogle Scholar
  18. 18.
    Ni PJ, Sun YJ, Dai HC, Lu WD, Jiang S, Wang YL, Li Z, Li Z (2017) Prussian blue nanocubes peroxidase mimetic-based colorimetric assay for screening acetylcholinesterase activity and its inhibitor. Sensors Actuators B Chem 240:1314–1320CrossRefGoogle Scholar
  19. 19.
    Jia SP, Zang JB, Li W, Tian PF, Zhou SY, Cai HX, Tian XQ, Wang YH (2018) A novel synthesis of Prussian blue nanocubes/biomass-derived nitrogen-doped porous carbon composite as a high-efficiency oxygen reduction reaction catalyst. Electrochim Acta 289:56–64CrossRefGoogle Scholar
  20. 20.
    Zhao BX, Huang Q, Dou LN, Bu T, Chen K, Yang QF, Yan LZ, Wang JL, Zhang DH (2018) Prussian blue nanoparticles based lateral flow assay for high sensitive determination of clenbuterol. Sensors Actuators B Chem 275:223–229CrossRefGoogle Scholar
  21. 21.
    Muthusamy S, Charles J, Renganathan B, Sastikumar D (2018) In situ growth of Prussian blue nanocubes on polypyrrole nanoparticles: facile synthesis, characterization and their application as fiber optic gas sensor. J Mater Sci 53:15401–15417CrossRefGoogle Scholar
  22. 22.
    Han J, Zhuo Y, Chai YQ, Yuan R, Xiang Y, Zhu Q, Liao N (2013) Multi-labeled functionalized C60 nanohybrid as tracing tag for ultrasensitive electrochemical aptasensing. Biosens Bioelectron 46:74–79CrossRefGoogle Scholar
  23. 23.
    Zhuo Y, Yuan PX, Yuan R, Chai YQ, Hong CL (2009) Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors. Biomaterials 30:2284–2290CrossRefGoogle Scholar
  24. 24.
    Cui L, Hu J, Li CC, Wang CM, Zhang CY (2018) An electrochemical biosensor based on the enhanced quasi-reversible redox signal of Prussian blue generated by self-sacrificial label of Iron metal-organic framework. Biosens Bioelectron 122:168–174CrossRefGoogle Scholar
  25. 25.
    Zhang WM, Ma D, Du JX (2014) Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose. Talanta 120:362–367CrossRefGoogle Scholar
  26. 26.
    Xing HH, Zhang XW, Zhai QF, Li J, Wang EK (2017) Bipolar electrode-based reversible fluorescence switch using Prussian blue/Au nanoclusters nanocomposite film. Anal Chem 89:3867–3872CrossRefGoogle Scholar
  27. 27.
    Karyakin AA, Karyakina EE (1999) Prussian blue-based 'artificial peroxidase'as a transducer for hydrogen peroxide detection. Application to biosensors. Sensors Actuators B Chem 57:268–273CrossRefGoogle Scholar
  28. 28.
    Huang W, Liang Y, Deng YQ, Cai YH, He Y (2017) Prussian blue nanoparticles as optical probes for visual and spectrophotometric determination of silver ions. Microchim Acta 184:2959–2964CrossRefGoogle Scholar
  29. 29.
    Fu GL, Liu W, Feng SS, Yue XL (2012) Prussian blue nanoparticles operate as a new generation of photothermal ablation agents for cancer therapy. Chem Commun 48:11567–11569CrossRefGoogle Scholar
  30. 30.
    Hu Q, He MH, Mei YQ, Feng WJ, Jing S, Kong JM, Zhang XJ (2017) Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex. Talanta 163:146–152CrossRefGoogle Scholar
  31. 31.
    Hu Q, Zhou BJ, Dang PY, Li LZ, Kong JM, Zhang XJ (2017) Facile colorimetric assay of alkaline phosphatase activity using Fe(II)-phenanthroline reporter. Anal Chim Acta 950:170–177CrossRefGoogle Scholar
  32. 32.
    Yang JJ, Zheng L, Wang Y, Li W, Zhang JL, Gu JJ, Fu Y (2016) Guanine-rich DNA-based peroxidase mimetics for colorimetric assays of alkaline phosphatase. Biosens Bioelectron 77:549–556CrossRefGoogle Scholar
  33. 33.
    Liu HJ, Li M, Xia YN, Ren XQ (2016) A turn-on fluorescent sensor for selective and sensitive detection of alkaline phosphatase activity with gold nanoclusters based on inner filter effect. ACS Appl Mater Interfaces 9:120–126CrossRefGoogle Scholar
  34. 34.
    Liu JJ, Tang DS, Chen ZT, Yan XM, Zhong Z, Kang LT, Yao JN (2017) Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase. Biosens Bioelectron 94:271–277CrossRefGoogle Scholar
  35. 35.
    Mei YQ, Hu Q, Zhou BJ, Zhang YH, He MH, Xu T, Li F, Kong JM (2017) Fluorescence quenching based alkaline phosphatase activity detection. Talanta 176:52–58CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical EngineeringHunan Normal UniversityChangshaPeople’s Republic of China
  2. 2.College of ScienceHunan Agricultural UniversityChangshaPeople’s Republic of China

Personalised recommendations