Microchimica Acta

, 186:90 | Cite as

A glassy carbon electrode modified with graphene oxide, poly(3,4-ethylenedioxythiophene), an antifouling peptide and an aptamer for ultrasensitive detection of adenosine triphosphate

  • Zhenjiang Li
  • Jifang Yin
  • Chenghai Gao
  • Liying Sheng
  • Alan MengEmail author
Original Paper


An antifouling aptasensor is described for voltammetric determination of adenosine triphosphate (ATP). A glassy carbon electrode (GCE) was modified with a graphene oxide and poly(3,4-ethylenedioxythiophene) (GO-PEDOT) composite film by electrodeposition. Next, the zwitterionic peptide (EKEKEKE) was attached. It forms an antifouling layer on the modified GCE and serves as the support for subsequent aptamer immobilization. The resulting aptasensor typically is operated at a potential of 0.18 V (vs. SCE) using hexacyanoferrate as the electrochemical probe. It has a linear response in the 0.1 pM to 1.0 μM ATP concentration range, a 0.03 pM detection limit and a sensitivity of 2674.7 μA·μM−1·cm−2. It has outstanding selectivity, satisfactory reproducibility and desired stability. It was used to quantify ATP in ATP-spiked 10% serum solutions.

Graphical abstract

Schematic presentation of the construction of the aptamer based electrode for voltammetric determination of ATP.


Adenosine triphosphate GO-PEDOT composite film Zwitterionic peptide Electrochemical aptasensor Electrodeposition Contact angle Antifouling ability Aptasensor Clinical analysis 



The work reported here was supported by the National Natural Science Foundation of China under Grant No.51672144, 51572137, 51502149, 51702181, the Natural Science Foundation of Shandong Province under Grant No. ZR2016EMB25, ZR2017PEM006, ZR2017BB013, the Higher Educational Science and Technology Program of Shandong Province under Grant No.J16LA10, J17KA014, the Application Foundation Research Program of Qingdao under Grant No. 15-9-1-28-jch, the Taishan Scholars Program of Shandong Province under No. ts201511034 and the Overseas Taishan Scholars Program. We express our grateful thanks to them for their financial support.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3211_MOESM1_ESM.docx (417 kb)
ESM 1 (DOCX 416 kb)


  1. 1.
    Ding XJ, Wang YH, Cheng W, Mo F, Sang Y, Xu LL, Ding S (2017) Aptamer based electrochemical adenosine triphosphate assay based on a target-induced dendritic DNA nanoassembly. Microchim Acta 184:431–438CrossRefGoogle Scholar
  2. 2.
    Lee T, Seeman P, Rajput A, Farley IJ, Hornykiewicz O (1978) Receptor basis for dopaminergic supersensitivity in Parkinson's disease. Nature 273:59–61CrossRefGoogle Scholar
  3. 3.
    Yokoshiki H, Sunagawa M, Seki T, Sperelakis N (1998) ATP-sensitive K+ channels in pancreatic, cardiac, and vascular smooth muscle cells. Am J Phys Cell Phys 274:C25–C37CrossRefGoogle Scholar
  4. 4.
    Tang L, Chun IS, Wang Z, Li JH, Li XL, Lu Y (2013) DNA detection using Plasmonic enhanced near-infrared photoluminescence of gallium arsenide. Anal Chem 85:9522–9527CrossRefGoogle Scholar
  5. 5.
    Wang J, Wang LH, Liu XF, Liang ZQ, Song SP, Li WX, Li GX, Fan CH (2007) A gold nanoparticle-based aptamer target binding readout for ATPAssay. Adv Mater 19:3943–3946CrossRefGoogle Scholar
  6. 6.
    Zuo XL, Song SP, Zhang J, Pan D, Wang LH, Fan CH (2007) Target-responsive electrochemical aptamer switch (TREAS) for Reagentless detection of Nanomolar ATP. J Am Chem Soc 129:1042–1043CrossRefGoogle Scholar
  7. 7.
    Liu X, Liu HW, Li M, Qi HL, Gao Q, Zhang CX (2017) Highly sensitive Electrochemiluminescence assay for cardiac troponin I and adenosine triphosphate by using Supersandwich amplification and bifunctional aptamer. Electronal Chem 4:1708–1713Google Scholar
  8. 8.
    Tang DP, Hou L (2016) Aptasensor for ATP based on analyte-induced dissociation of ferrocene-aptamer conjugates from manganese dioxide nanosheets on a screen-printed carbon electrode. Microchim Acta 183:2705–2711CrossRefGoogle Scholar
  9. 9.
    Shamsipur M, Farzin L, Tabrizi MA, Shanehsaz M (2016) CdTe amplification nanoplatforms capped with thioglycolic acid for electrochemical aptasensing of ultra-traces of ATP. Mater Sci Eng C 69:1354–1360CrossRefGoogle Scholar
  10. 10.
    Shi PF, Zhang YC, Yu ZP, Zhang SS (2017) Label-free electrochemical detection of ATP based on amino-functionalized metal-organic framework. Sci Rep 7:1–7CrossRefGoogle Scholar
  11. 11.
    Pei SF, Cheng HM (2012) The reduction of graphene oxide. Carbon 50:3210–3228CrossRefGoogle Scholar
  12. 12.
    Luo XL, Weaver CL, Tan SS, Cui XY (2013) Pure graphene oxide doped conducting polymer nanocomposite for bio-interfacing. J Mater Chem B 1:1340–1348CrossRefGoogle Scholar
  13. 13.
    Cheng X, Cen Y, Xu GH, Wei FD, Shi ML, Xu XM, Sohail M, Hu Q (2018) Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide. Microchim Acta 185:144–152CrossRefGoogle Scholar
  14. 14.
    Li SQ, Fu YW, Ma XJ, Zhang YD (2017) Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials. Biosens Bioelectron 88:210–216CrossRefGoogle Scholar
  15. 15.
    Sheng LY, Li ZJ, Meng AL, Xu QH (2018) Ultrafast responsive and highly sensitive enzyme-free glucose sensor based on a novel Ni(OH)2@PEDOT-rGO nanocomposite. Sensors Actuators B Chem 254:1206–1215CrossRefGoogle Scholar
  16. 16.
    Sakthivel M, Sivakumar M, Chen SM, Pandi K (2018) Electrochemical synthesis of poly(3,4-ethylenedioxythiophene) on terbium hexacyanoferrate for sensitive determination of tartrazine. Sensors Actuators B Chem 256:195–203CrossRefGoogle Scholar
  17. 17.
    Hryniewicz BM, Orth ES, Vidotti M (2018) PEDOT-based electrochemical sensor for the detection of nitrophenols and organophosphates. Sensors Actuators B Chem 257:570–578CrossRefGoogle Scholar
  18. 18.
    Wang GX, Han R, Su XL, Li Y, Xu GY, Luo XL (2017) Zwitterionic peptide anchored to conducting polymer PEDOT for the development of antifouling and ultrasensitive electrochemical DNA sensor. Biosens Bioelectron 92:396–401CrossRefGoogle Scholar
  19. 19.
    Chernousova S, Epple M (2013) Silver as antibacterial agent: ion, nanoparticle, and metal. Angew Chem Int Ed 52:1636–1653CrossRefGoogle Scholar
  20. 20.
    Tischer M, Pradel G, Ohlsen K, Holzgrabe U (2012) Quaternary ammonium salts and their antimicrobial potential: targets or nonspecific interactions. ChemMedChem 7:22–31CrossRefGoogle Scholar
  21. 21.
    Zhang PC, Lin L, Zang DM, Guo XL, Liu MJ (2017) Designing bioinspired anti-biofouling surfaces based on a Superwettability strategy. Small 13:1–9Google Scholar
  22. 22.
    Shao Q, Jiang SY (2015) Molecular understanding and Design of Zwitterionic Materials. Adv Mater 27:15–26CrossRefGoogle Scholar
  23. 23.
    Sun QQ, Yan F, Yao YN, Su B (2016) Anti-biofouling Isoporous silica-micelle membrane enabling drug detection in human whole blood. Anal Chem 88:8364–8368CrossRefGoogle Scholar
  24. 24.
    Lowe S, O’Brien-Simpson NM, Connal LA (2015) Antibiofouling polymer interfaces: poly(ethylene glycol) and other promising candidates. Polym Chem 6:198–212CrossRefGoogle Scholar
  25. 25.
    Liu NZ, Hui N, Davis JJ, Luo XL (2018) Low fouling protein detection in complex biological media supported by a designed multifunctional peptide. ACS Sens 3:1210–1216CrossRefGoogle Scholar
  26. 26.
    Wang GX, Su XL, Xu QJ, Xu GY, Lin JH, Luo XL (2018) Antifouling aptasensor for the detection of adenosine triphosphate in biological media based on mixed self-assembled aptamer and zwitterionic peptide. Biosens Bioelectron 101:129–134CrossRefGoogle Scholar
  27. 27.
    Meng AL, Sheng LY, Zhao K, Li ZJ (2017) A controllable honeycomb-like amorphous cobalt sulfide architecture directly grown on the reduced graphene oxide–poly(3,4-ethylenedioxythiophene) composite through electrodeposition for non-enzyme glucose sensing. J Mater Chem B 5:8934–8943CrossRefGoogle Scholar
  28. 28.
    Xu Q, Pu P, Zhao JG, Dong CB, Gao C, Chen YS, Chen JR, Liu Y, Zhou HJ (2015) Preparation of highly photoluminescent sulfur-doped carbon dots for Fe( III ) detection. J Mater Chem A 3:542–546CrossRefGoogle Scholar
  29. 29.
    Liu XJ, Lin BX, Yu Y, Cao YJ, Guo ML (2018) A multifunctional probe based on the use of labeled aptamer and magnetic nanoparticles for fluorometric determination of adenosine 5′-triphosphate. Microchim Acta 185:243–251CrossRefGoogle Scholar
  30. 30.
    Xie H, Chai YQ, Yuan YL, Yuan R (2017) Highly effective molecule converting strategy based on enzyme-free dual recycling amplification for ultrasensitive electrochemical detection of ATP. Chem Commun 53:8368–8371CrossRefGoogle Scholar
  31. 31.
    Chen JY, Liu YC, Ji XH, He ZK (2016) Target-protecting dumbbell molecular probe against exonucleases digestion for sensitive detection of ATP and streptavidin. Biosens Bioelectron 83:221–228CrossRefGoogle Scholar
  32. 32.
    Hai XM, Li N, Wang K, Zhang ZQ, Zhang J, Dang FQ (2018) A fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks for monitoring adenosine triphosphate. Anal Chim Acta 998:60–66CrossRefGoogle Scholar
  33. 33.
    Song YH, Yang X, Li ZQ, Zhao YJ, Fan AP (2014) Label-free chemiluminescent ATP aptasensor based on graphene oxide and an instantaneous derivatization of guanine bases. Biosens Bioelectron 51:232–237CrossRefGoogle Scholar
  34. 34.
    Santangelo MF, Libertino S, Turner APF, Filippini D, Mak WC (2018) Integrating printed microfluidics with silicon photomultipliers for miniaturised and highly sensitive ATP bioluminescence detection. Biosens Bioelectron 99:464–470CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Zhenjiang Li
    • 1
  • Jifang Yin
    • 2
  • Chenghai Gao
    • 2
  • Liying Sheng
    • 1
  • Alan Meng
    • 3
    Email author
  1. 1.Key Laboratory of Polymer Material Advanced Manufacturing Technology of Shandong Provincial, College of Electromechanical Engineering, College of Sino-German Science and TechnologyQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  2. 2.College of Materials Science and EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  3. 3.Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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