Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid
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A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO2@BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO2@BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO2@BSA recovers its fluorescence because MnO2@BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO2 and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.
KeywordsBiomimetic approach Ascorbic acid Dual-modal detection
This work was supported by the National Natural Science Foundation of China (81571742, 81871399, 81801823), Project funded by China Postdoctoral Science Foundation (1500229020), Shanghai Innovation Program (14ZZ039), Program for Outstanding Young Teachers in Tongji University, and the Fundamental Research Funds for the Central Universities. International Science & Technology Cooperation Program of China (2014DFA33010).
Compliance with ethical standards
The author(s) declare that they have no competing interests
- 10.Zhao P, He KY, Han YT, Zhang Z, Yu MZ, Wang HH, Huang Y, Nie Z, Yao SZ (2015) Near-infrared dual-emission quantum dots-gold nanoclusters nanohybrid via co-template synthesis for ratiometric fluorescent detection and bioimaging of ascorbic acid in vitro and in vivo. Anal Chem 87(19):9998–10005PubMedCrossRefGoogle Scholar
- 32.Zheng Y, Feng G, Shang T, Wu W, Huang J, Sun D, Wang Y, Li Q (2016) Separation of biosynthesized gold nanoparticles by density gradient centrifugation. Sep Sci 52(2):951–957Google Scholar
- 34.Zhao ZH, Wang XM, Zhang ZJ, Zhang H, Liu HY, Zhu XL, Li H, Chi XQ, Yin ZY, Gao JH (2015) Real-time monitoring of arsenic trioxide release and delivery by activatable T(1) imaging. ACS Nano 12(2):546–546Google Scholar