Microchimica Acta

, 186:89 | Cite as

Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid

  • Jiani Yu
  • Weitao Yang
  • Shige Xing
  • Jun Wang
  • Huanxing Han
  • Pengfei Zhang
  • Chenyang Xiang
  • Bingbo ZhangEmail author
Original Paper


A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO2@BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO2@BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO2@BSA recovers its fluorescence because MnO2@BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO2 and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.

Graphical abstract

Schematic presentation of the biomimetic synthesis of blended Au/MnO2@BSA nanoprobes and fluorometric/MR cross-validation dual-modal detection of ascorbic acid.


Biomimetic approach Ascorbic acid Dual-modal detection 



This work was supported by the National Natural Science Foundation of China (81571742, 81871399, 81801823), Project funded by China Postdoctoral Science Foundation (1500229020), Shanghai Innovation Program (14ZZ039), Program for Outstanding Young Teachers in Tongji University, and the Fundamental Research Funds for the Central Universities. International Science & Technology Cooperation Program of China (2014DFA33010).

Compliance with ethical standards

The author(s) declare that they have no competing interests

Supplementary material

604_2018_3205_MOESM1_ESM.docx (4.1 mb)
ESM 1 (DOCX 4.13 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Photomedicine, Shanghai Skin Disease Hospital, The Institute for Biomedical Engineering & Nano ScienceTongji University School of MedicineShanghaiPeople’s Republic of China
  2. 2.Chongqing University of EducationChongqingPeople’s Republic of China
  3. 3.Institute of Food SafetyChinese Academy of Inspection & QuarantineBeijingPeople’s Republic of China
  4. 4.Department of Pharmacy, Changzheng HospitalSecond Military Medical UniversityShanghaiPeople’s Republic of China

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