Microchimica Acta

, 186:101 | Cite as

An amino-modified metal-organic framework (type UiO-66-NH2) loaded with cadmium(II) and lead(II) ions for simultaneous electrochemical immunosensing of triazophos and thiacloprid

  • Yong Yang
  • Jingli Cheng
  • Biao Wang
  • Yirong Guo
  • Xiaowu Dong
  • Jinhao ZhaoEmail author
Original Paper


A method is described for simultaneous voltammetric determination of the pesticides triazophos (TRS) and thiacloprid (THD). A glassy carbon electrode (GCE) was modified with a metal-organic framework (type UiO-66-NH2) which has a large specific surface (1018 m2·g−1) and contains large amounts of Cd(II) and Pb(II) ions, with adsorption capacities of 230 and 271 mg·g−1, respectively. The antigen-loaded particles were then bound to antibody, magnetically separated, and analyzed by square wave voltammetry to give signals for Cd(II) and Pb(II) at −0.82 and − 0.56 V (vs. Ag/AgCl) for TRS and THD, respectively. Under optimized conditions, the method has a wide linear range (0.2–750 ng·mL−1) and low detection limits (0.07 and 0.1 ng·mL−1 at a S/N of 3 for TRS and THD, respectively). It is perceived that this assay represents a useful tool for simultaneous determination of multiple pesticide residues. The method has a wide scope in that may be extended to monitoring of other small organic pollutants by changing the types of metal ions and by using other antibodies.

Graphical abstract

Schematic presentation of an amino-modified metal-organic framework (type UiO-66-NH2) loaded with Cd(II) and Pb(II) ions for simultaneous electrochemical immunosensing of triazophos (TRS) and thiacloprid (THD). It is based on the fabrication of antigen (Ab)-immobilized UiO-66-NH2-based signal tags (a), and of an antibody (Ab)-immobilized magnetic bead (MB-COOH)-based capture probes (b).


MOF UiO-66-NH2 Simultaneous voltammetric determination Electrochemical immunosensing Multiple pesticide residues Square wave voltammetry Cd(II) ion Pb(II) ion 



This work was supported financially by the Experimental Technology Research Project of Zhejiang University (No. SJS201710) and the National Key Research and Development Program of China (2016YFD0200804, 2017YFF0210200, 2017YFD0201805).

Compliance with ethical standards

The author(s) declare that they have no competing interest.

Supplementary material

604_2018_3201_MOESM1_ESM.docx (19.1 mb)
ESM 1 (DOCX 19.0 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Yong Yang
    • 1
  • Jingli Cheng
    • 1
  • Biao Wang
    • 1
  • Yirong Guo
    • 1
  • Xiaowu Dong
    • 2
  • Jinhao Zhao
    • 1
    Email author
  1. 1.Institute of Pesticide and Environmental Toxicology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and InsertsZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Hangzhou Institute of Innovative Medicine, College of Pharmaceutical SciencesZhejiang UniversityHangzhouPeople’s Republic of China

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