An electrochemical aptasensor for streptomycin based on covalent attachment of the aptamer onto a mesoporous silica thin film-coated gold electrode
- 105 Downloads
An electrochemical method is described for the determination of streptomycin (STR). It is making use of a gold electrode coated with a thin mesoporous silica film (MSF). In addition, silver nanoparticles were coated on the MSF to increase the surface area, to bind a large amount of aptamer (Apt), and to improve the electrical conductivity. In the presence of STR, it will bind to the Apt and hinder the diffusion of the redox probe hexacyanoferrate through the nanochannels of the mesoporous film. The aptasensor, best operated at a working potential of 0.22 V (vs. Ag/AgCl) has a linear response in the 1 fg.mL−1 to 6.2 ng.mL−1 STR concentration range. The detection limit is 0.33 fg.mL−1. The assay was successfully validated by analyzing spiked samples of milk and blood serum.
KeywordsSilver nanoparticles Hexacyanoferrate Differential pulse voltammetry Tetraethoxysilane Cetyltrimethylammonium bromide
The authors thank the Iran National Science Foundation (Grant no. 96015612) for their support.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 15.Danesh NM, Ramezani M, Emrani AS, Abnous K, Taghdis SM (2015) A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin. Biosens Bioelectron 15:123–128Google Scholar
- 24.Valipour A, Roushani M (2017) Using silver nanoparticle and thiol graphene quantum dots nanocomposite as a substratum to load antibody for detection of hepatitis C virus core antigen: electrochemical oxidation of riboflavin was used as redox probe. Biosens Bioelectron 89:946–951CrossRefGoogle Scholar
- 25.Ogawa M (2001) Mesoporous silica films by supramolecular templating approach. Curr Top Colloid Interface Sci 4:209–217Google Scholar