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Microchimica Acta

, 186:78 | Cite as

Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots

  • Qiyong CaiEmail author
  • Hongmin Meng
  • Yeru Liu
  • Zhaohui LiEmail author
Original Paper
  • 131 Downloads

Abstract

A method is described for fluorometric detection of glucose. It is based on the finding that silicon nanodots (SNDs) are formed from glucose and aminopropyltriethoxysilane (APTES) under mild experimental conditions. The SNDs thus formed have an average diameter of ∼2 nm, exhibit good water dispersibility, blue fluorescence (with excitation/emission maxima at 410/475 nm), broad pH tolerance, and are photostable. The assay was applied to the quantification of glucose with high sensitivity, good specificity, and over a wide detection range (from 10 μM to 0.9 mM). It was applied to the determination of glucose in spiked serum samples and gave satisfactory results and recoveries.

Graphical abstract

Schematic presentation of serum glucose detection based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots.

Keywords

Silicon nanoparticles Diabetes Fluorescence detection Enzyme-free analysis 

Notes

Acknowledgements

This work was supported in part by the National Natural Science Foundation of China (21205108), the Foundation for University Key Teacher by Henan Province (2017GGJS007), the Key Scientific Research Project in Universities of Henan Province (19A150048), and the Fundamental Research Funds for the Central Universities from Hunan University.

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2018_3189_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1989 kb)

References

  1. 1.
    Li J, Li Y, Shahzad SA, Chen J, Chen Y, Wang Y, Yang M, Yu C (2015) Fluorescence turn-on detection of glucose via the ag nanoparticle mediated release of a perylene probe. Chem Commun 51(29):6354–6356CrossRefGoogle Scholar
  2. 2.
    Hu Y, Cheng H, Zhao X, Wu J, Muhammad F, Lin S, He J, Zhou L, Zhang C, Deng Y, Wang P, Zhou Z, Nie S, Wei H (2017) Surface-enhanced Raman scattering active gold nanoparticles with enzyme-mimicking activities for measuring glucose and lactate in living tissues. ACS Nano 11(6):5558–5566CrossRefGoogle Scholar
  3. 3.
    Wang Z, Cao X, Liu D, Hao S, Du G, Asiri AM, Sun X (2016) Ternary NiCoP nanosheet array on a Ti mesh: a high-performance electrochemical sensor for glucose detection. Chem Commun 52(100):14438–14441CrossRefGoogle Scholar
  4. 4.
    Grembecka M, Lebiedzińska A, Szefer P (2014) Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector. Microchem J 117:77–82CrossRefGoogle Scholar
  5. 5.
    Wu Q, Wang X, Liao C, Wei Q, Wang Q (2015) Microgel coating of magnetic nanoparticles via bienzyme-mediated free-radical polymerization for colorimetric detection of glucose. Nanoscale 7(40):16578–16582CrossRefGoogle Scholar
  6. 6.
    Jin L, Meng Z, Zhang Y, Cai S, Zhang Z, Li C, Shang L, Shen Y (2017) Ultrasmall Pt nanoclusters as robust peroxidase mimics for colorimetric detection of glucose in human serum. ACS Appl Mater Interfaces 9(11):10027–10033CrossRefGoogle Scholar
  7. 7.
    Schügerl K, Hitzmann B, Jurgens H, Kullick T, Ulber R, Weigal B (1996) Challenges in integrating biosensors and FIA for on-line monitoring and control. Trends Biotechnol 14(1):21–31CrossRefGoogle Scholar
  8. 8.
    Wei H, Wang E (2013) Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem Soc Rev 42(14):6060–6093CrossRefGoogle Scholar
  9. 9.
    Ying H, Meiting Z, Shikui H, Zhuangchai L, Jian Y, Chaoliang T, Qinglang M, Qipeng L, Junze C, Xiao Z, Zhicheng Z, Bing L, Bo C, Yun Z, Hua Z (2017) Growth of au nanoparticles on 2D Metalloporphyrinic metal-organic framework Nanosheets used as biomimetic catalysts for Cascade reactions. Adv Mater 29(32):1700102CrossRefGoogle Scholar
  10. 10.
    David R (2016) Continuous glucose monitoring: a review of successes, challenges, and opportunities. Diabetes Technol Ther 18(S2):S2–3-S2-13CrossRefGoogle Scholar
  11. 11.
    Dhara K, Mahapatra DR (2018) Electrochemical nonenzymatic sensing of glucose using advanced nanomaterials. Microchim Acta 185(1):49CrossRefGoogle Scholar
  12. 12.
    Rahman MM, Ahammad AJS, Jin J-H, Ahn SJ, Lee J-J (2010) A comprehensive review of glucose biosensors based on nanostructured metal-oxides. Sensors 10(5):4855–4886CrossRefGoogle Scholar
  13. 13.
    Steiner M-S, Duerkop A, Wolfbeis OS (2011) Optical methods for sensing glucose. Chem Soc Rev 40(9):4805–4839CrossRefGoogle Scholar
  14. 14.
    Shen P, Xia Y (2014) Synthesis-modification integration: one-step fabrication of Boronic acid functionalized carbon dots for fluorescent blood sugar sensing. Anal Chem 86(11):5323–5329CrossRefGoogle Scholar
  15. 15.
    Z-b Q, Zhou X, Gu L, Lan R, Sun D, Yu D, Shi G (2013) Boronic acid functionalized graphene quantum dots as a fluorescent probe for selective and sensitive glucose determination in microdialysate. Chem Commun 49(84):9830–9832CrossRefGoogle Scholar
  16. 16.
    Chandra S, Masuda Y, Shirahata N, Winnik FM (2017) Transition-metal-doped NIR-emitting silicon nanocrystals. Angew Chem Int Ed 56(22):6157–6160CrossRefGoogle Scholar
  17. 17.
    Cheng X, Lowe SB, Reece PJ, Gooding JJ (2014) Colloidal silicon quantum dots: from preparation to the modification of self-assembled monolayers (SAMs) for bio-applications. Chem Soc Rev 43(8):2680–2700CrossRefGoogle Scholar
  18. 18.
    Croissant JG, Cattoen X, Durand J-O, Wong Chi Man M, Khashab NM (2016) Organosilica hybrid nanomaterials with a high organic content: syntheses and applications of silsesquioxanes. Nanoscale 8(48):19945–19972CrossRefGoogle Scholar
  19. 19.
    C J G, Yevhen F, Abdulaziz A, K N M (2018) Mesoporous silica and Organosilica nanoparticles: physical chemistry, biosafety, delivery strategies, and biomedical applications. Adv Healthc Mater 7(4):1700831CrossRefGoogle Scholar
  20. 20.
    Miller JB, Dandu N, Velizhanin KA, Anthony RJ, Kortshagen UR, Kroll DM, Kilina S, Hobbie EK (2015) Enhanced luminescent stability through particle interactions in silicon nanocrystal aggregates. ACS Nano 9(10):9772–9782CrossRefGoogle Scholar
  21. 21.
    McVey BFP, Tilley RD (2014) Solution synthesis, optical properties, and bioimaging applications of silicon nanocrystals. Acc Chem Res 47(10):3045–3051CrossRefGoogle Scholar
  22. 22.
    Zhang H, Zhang Q, Li M, Kan B, Ni W, Wang Y, Yang X, Du C, Wan X, Chen Y (2015) Investigation of the enhanced performance and lifetime of organic solar cells using solution-processed carbon dots as the electron transport layers. J Mater Chem C 3(48):12403–12409CrossRefGoogle Scholar
  23. 23.
    Liu H, Li Z, Sun Y, Geng X, Hu Y, Meng H, Ge J, Qu L (2018) Synthesis of luminescent carbon dots with ultrahigh quantum yield and inherent folate receptor-positive Cancer cell Targetability. Sci Rep 8(1):1086CrossRefGoogle Scholar
  24. 24.
    Hu Y, Geng X, Zhang L, Huang Z, Ge J, Li Z (2017) Nitrogen-doped carbon dots mediated fluorescent on-off assay for rapid and highly sensitive pyrophosphate and alkaline phosphatase detection. Sci Rep 7(1):5849CrossRefGoogle Scholar
  25. 25.
    Taguchi T, Miwa I, Mizutani T, Nakajima H, Fukumura Y, Kobayashi I, Yabuuchi M, Miwa I (2003) Determination of D-Mannose in Plasma by HPLC 49(1):181–183Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityChangshaPeople’s Republic of China
  2. 2.College of Chemistry and Molecular EngineeringZhengzhou UniversityZhengzhouPeople’s Republic of China

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