Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots
- 47 Downloads
A method is described for fluorometric detection of glucose. It is based on the finding that silicon nanodots (SNDs) are formed from glucose and aminopropyltriethoxysilane (APTES) under mild experimental conditions. The SNDs thus formed have an average diameter of ∼2 nm, exhibit good water dispersibility, blue fluorescence (with excitation/emission maxima at 410/475 nm), broad pH tolerance, and are photostable. The assay was applied to the quantification of glucose with high sensitivity, good specificity, and over a wide detection range (from 10 μM to 0.9 mM). It was applied to the determination of glucose in spiked serum samples and gave satisfactory results and recoveries.
KeywordsSilicon nanoparticles Diabetes Fluorescence detection Enzyme-free analysis
This work was supported in part by the National Natural Science Foundation of China (21205108), the Foundation for University Key Teacher by Henan Province (2017GGJS007), the Key Scientific Research Project in Universities of Henan Province (19A150048), and the Fundamental Research Funds for the Central Universities from Hunan University.
Compliance with ethical standards
The authors declare that they have no competing interests.
- 2.Hu Y, Cheng H, Zhao X, Wu J, Muhammad F, Lin S, He J, Zhou L, Zhang C, Deng Y, Wang P, Zhou Z, Nie S, Wei H (2017) Surface-enhanced Raman scattering active gold nanoparticles with enzyme-mimicking activities for measuring glucose and lactate in living tissues. ACS Nano 11(6):5558–5566CrossRefGoogle Scholar
- 4.Grembecka M, Lebiedzińska A, Szefer P (2014) Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector. Microchem J 117:77–82CrossRefGoogle Scholar
- 9.Ying H, Meiting Z, Shikui H, Zhuangchai L, Jian Y, Chaoliang T, Qinglang M, Qipeng L, Junze C, Xiao Z, Zhicheng Z, Bing L, Bo C, Yun Z, Hua Z (2017) Growth of au nanoparticles on 2D Metalloporphyrinic metal-organic framework Nanosheets used as biomimetic catalysts for Cascade reactions. Adv Mater 29(32):1700102CrossRefGoogle Scholar
- 25.Taguchi T, Miwa I, Mizutani T, Nakajima H, Fukumura Y, Kobayashi I, Yabuuchi M, Miwa I (2003) Determination of D-Mannose in Plasma by HPLC 49(1):181–183Google Scholar