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Microchimica Acta

, 186:77 | Cite as

Voltammetric kanamycin aptasensor based on the use of thionine incorporated into Au@Pt core-shell nanoparticles

  • Baoshan HeEmail author
  • Sasa Yan
Original Paper

Abstract

A signal-on aptasensor is described for the voltammetric determination of kanamycin (KANA). Au@Pt core-shell nanoparticles with large surface and good electrical conductivity were synthetized and act as both a conductive material and as the carrier for complementary strands (CS2) and thionine (TH). In the presence of KANA, the electrochemical response of TH changes due to hybridization between CS1 immobilized on the electrode and the Au@Pt-CS2/TH system. The peak current increases linearly with the logarithm of the KANA concentration in the range from 1 pM to 1 μM, and the limit of detection is 0.16 pM. The sensor was characterized in terms of selectivity, reproducibility and stability, and satisfactory results were obtained. It was also utilized for the determination of KANA in (spiked) chicken samples. The recoveries (95.8–103.2%) demonstrate the potential of the method for KANA detection in real samples.

Graphical abstract

A signal-on aptasensor for kanamycin (KANA) was developed by using Au@Pt core-shell nanoparticles as nanocarrier for probe aptamer and as a sensing probe.

Keywords

Kanamycin (KANA) Aptamer Electrochemical detection Signal-on Aptasensor Chicken sample 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 61301037), the Henan Science and Technology Cooperation Project (Grant No. 172106000014), the Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), the Youth Backbone Teacher Training Program of Henan University of Technology (No. 21420004), and the Master's Degree Thesis Cultivation Project of Henan University of Technology.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3188_MOESM1_ESM.doc (814 kb)
ESM 1 (DOC 814 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou High & New Technology Industries Development ZoneHenan University of TechnologyZhengzhouPeople’s Republic of China

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