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Voltammetric kanamycin aptasensor based on the use of thionine incorporated into Au@Pt core-shell nanoparticles

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Abstract

A signal-on aptasensor is described for the voltammetric determination of kanamycin (KANA). Au@Pt core-shell nanoparticles with large surface and good electrical conductivity were synthetized and act as both a conductive material and as the carrier for complementary strands (CS2) and thionine (TH). In the presence of KANA, the electrochemical response of TH changes due to hybridization between CS1 immobilized on the electrode and the Au@Pt-CS2/TH system. The peak current increases linearly with the logarithm of the KANA concentration in the range from 1 pM to 1 μM, and the limit of detection is 0.16 pM. The sensor was characterized in terms of selectivity, reproducibility and stability, and satisfactory results were obtained. It was also utilized for the determination of KANA in (spiked) chicken samples. The recoveries (95.8–103.2%) demonstrate the potential of the method for KANA detection in real samples.

A signal-on aptasensor for kanamycin (KANA) was developed by using Au@Pt core-shell nanoparticles as nanocarrier for probe aptamer and as a sensing probe.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 61301037), the Henan Science and Technology Cooperation Project (Grant No. 172106000014), the Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), the Youth Backbone Teacher Training Program of Henan University of Technology (No. 21420004), and the Master's Degree Thesis Cultivation Project of Henan University of Technology.

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  1. School of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou High & New Technology Industries Development Zone, Henan University of Technology, Lianhua Road 100#, Zhengzhou, 450001, Henan Province, People’s Republic of China

    Baoshan He & Sasa Yan

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  1. Baoshan He
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  2. Sasa Yan
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Correspondence to Baoshan He.

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He, B., Yan, S. Voltammetric kanamycin aptasensor based on the use of thionine incorporated into Au@Pt core-shell nanoparticles. Microchim Acta 186, 77 (2019). https://doi.org/10.1007/s00604-018-3188-5

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