Voltammetric kanamycin aptasensor based on the use of thionine incorporated into Au@Pt core-shell nanoparticles
A signal-on aptasensor is described for the voltammetric determination of kanamycin (KANA). Au@Pt core-shell nanoparticles with large surface and good electrical conductivity were synthetized and act as both a conductive material and as the carrier for complementary strands (CS2) and thionine (TH). In the presence of KANA, the electrochemical response of TH changes due to hybridization between CS1 immobilized on the electrode and the Au@Pt-CS2/TH system. The peak current increases linearly with the logarithm of the KANA concentration in the range from 1 pM to 1 μM, and the limit of detection is 0.16 pM. The sensor was characterized in terms of selectivity, reproducibility and stability, and satisfactory results were obtained. It was also utilized for the determination of KANA in (spiked) chicken samples. The recoveries (95.8–103.2%) demonstrate the potential of the method for KANA detection in real samples.
KeywordsKanamycin (KANA) Aptamer Electrochemical detection Signal-on Aptasensor Chicken sample
This work was supported by the National Natural Science Foundation of China (Grant No. 61301037), the Henan Science and Technology Cooperation Project (Grant No. 172106000014), the Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), the Youth Backbone Teacher Training Program of Henan University of Technology (No. 21420004), and the Master's Degree Thesis Cultivation Project of Henan University of Technology.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 9.Arsand JB, Jank L, Martins MT, Hoff RB, Barreto F, Pizzolato TM (2016) Determination of aminoglycoside residues in milk and muscle based on a simple and fast extraction procedure followed by liquid chromatography coupled to tandem mass spectrometry and time of flight mass spectrometry. Talanta 154:38–45CrossRefGoogle Scholar
- 13.Fei X, Jing L, Zhou J, Liu M, Liu Y, Wang J (2016) A visual gel-enzyme-linked immunosorbent assay for simultaneous detection of gentamicin and kanamycin in milk. Chinese J Anal Chem 43:881–885Google Scholar
- 14.Su P, Chen X, He Z, Yang Y (2017) Preparation of polyclonal antibody and development of a biotin-streptavidin-based ELISA method for detecting kanamycin in milk and honey. Chinese J Anal Chem 33:1–6Google Scholar
- 16.Arya SK, Zhurauski P, Jolly P, M Batistuti R, Mulato M, Estrela P (2018) Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. Biosens Bioelectron 102:106–112Google Scholar