Microchimica Acta

, 186:73 | Cite as

Photoelectrochemical determination of the activity of alkaline phosphatase by using a CdS@graphene conjugate coupled to CoOOH nanosheets for signal amplification

  • Weisu Kong
  • Qingqing Tan
  • Haiyu Guo
  • Han Sun
  • Xia QinEmail author
  • Fengli QuEmail author
Original Paper


A method is described for photoelectrochemical determination of the activity of alkaline phosphatase (ALP). It employs an indium tin oxide (ITO) electrode modified a CdS quantum dots@graphene (CdS@GR) composite and hexagonal cobalt oxyhydroxide (CoOOH) nanosheets. The CdS@GR nanocomposite was synthesized by assembling the CdS quantum dots onto a GO film to receive a basic photocurrent response of the ITO. This is further improved by covering it with CoOOH nanosheets. Secondly, 2-phospho-L-ascorbic acid (AAP) is added as a substrate for ALP. Its hydrolysis yields ascorbic acid which reduces CoOOH to form cobalt(II) ion. As a result, the CoOOH nanosheets decompose. This is accompanied by a reduction of the photocurrent. The effect was used to design a selective and sensitive assay of determination of the activity of ALP. Under the optimized experimental conditions, response is linear in the 10 to 300 U·L−1ALP activity range. The detection limit is 1.5 U·L−1 at a signal-to-noise ratio of 3.

Graphical abstract

Indium tin oxide (ITO) was coted with CdS@graphene and CoOOH to obtain a material with superior photoelectrochemical properties. The detection of alkaline phosphatase (ALP) was accomplished by using 2-phospho-L-ascorbic acid (AAP) which is hydrolyzed by ALP to release ascorbic acid (AA) which reduces CoOOH to Co2+.


Photoelectrochemical sensor Alkaline phosphatase CdS@GR-CoOOH nanocomposite 2-Phospho-L-ascorbic acid Photon-to-electricity conversion 



The authors are grateful for the support of the National Natural Science Foundation of China (21775089), Outstanding Youth Foundation of Shandong Province (ZR2017JL010), Key Research and Development Program of Jining City (2018ZDGH032), and Project of Shandong Province Higher Educational Science and Technology Program (J18KA101).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3182_MOESM1_ESM.docx (703 kb)
ESM 1 (DOCX 703 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringQufu Normal UniversityQufuChina
  2. 2.School of Geography and TourismQufu Normal UniversityRizhaoChina

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