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Microchimica Acta

, 186:73 | Cite as

Photoelectrochemical determination of the activity of alkaline phosphatase by using a CdS@graphene conjugate coupled to CoOOH nanosheets for signal amplification

  • Weisu Kong
  • Qingqing Tan
  • Haiyu Guo
  • Han Sun
  • Xia QinEmail author
  • Fengli QuEmail author
Original Paper
  • 134 Downloads

Abstract

A method is described for photoelectrochemical determination of the activity of alkaline phosphatase (ALP). It employs an indium tin oxide (ITO) electrode modified a CdS quantum dots@graphene (CdS@GR) composite and hexagonal cobalt oxyhydroxide (CoOOH) nanosheets. The CdS@GR nanocomposite was synthesized by assembling the CdS quantum dots onto a GO film to receive a basic photocurrent response of the ITO. This is further improved by covering it with CoOOH nanosheets. Secondly, 2-phospho-L-ascorbic acid (AAP) is added as a substrate for ALP. Its hydrolysis yields ascorbic acid which reduces CoOOH to form cobalt(II) ion. As a result, the CoOOH nanosheets decompose. This is accompanied by a reduction of the photocurrent. The effect was used to design a selective and sensitive assay of determination of the activity of ALP. Under the optimized experimental conditions, response is linear in the 10 to 300 U·L−1ALP activity range. The detection limit is 1.5 U·L−1 at a signal-to-noise ratio of 3.

Graphical abstract

Indium tin oxide (ITO) was coted with CdS@graphene and CoOOH to obtain a material with superior photoelectrochemical properties. The detection of alkaline phosphatase (ALP) was accomplished by using 2-phospho-L-ascorbic acid (AAP) which is hydrolyzed by ALP to release ascorbic acid (AA) which reduces CoOOH to Co2+.

Keywords

Photoelectrochemical sensor Alkaline phosphatase CdS@GR-CoOOH nanocomposite 2-Phospho-L-ascorbic acid Photon-to-electricity conversion 

Notes

Acknowledgments

The authors are grateful for the support of the National Natural Science Foundation of China (21775089), Outstanding Youth Foundation of Shandong Province (ZR2017JL010), Key Research and Development Program of Jining City (2018ZDGH032), and Project of Shandong Province Higher Educational Science and Technology Program (J18KA101).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3182_MOESM1_ESM.docx (703 kb)
ESM 1 (DOCX 703 kb)

References

  1. 1.
    Tan Y, Zhang L, Man KH, Peltier R, Chen GC, Zhang HT, Zhou LY, Wang F, Shao DH, Yao Q, Hu Y, Sun HY (2017) Reaction-based off−on near-infrared fluorescent probe for imaging alkaline phosphatase activity in living cells and mice. ACS Appl Mater Interfaces 9:6796–6803CrossRefGoogle Scholar
  2. 2.
    Guo LY, Chen DL, Yang MH (2017) DNA-templated silver nanoclusters for fluorometric determination of the activity and inhibition of alkaline phosphatase. Microchim Acta 184:2165–2170CrossRefGoogle Scholar
  3. 3.
    Kang KY, Hong YS, Park SH, Ju JH (2015) Increased serum alkaline phosphatase levels correlate with high disease activity and low bone mineral density in patients with axial spondyloarthritis. Semin Arthritis Rheum 45:202–207CrossRefGoogle Scholar
  4. 4.
    Kang Q, Wang XX, Ma XL, Kong LQ, Zhang P, Shen DZ (2016) Sensitive detection of ascorbic acid and alkaline phosphatase activity by double-channel photoelectrochemical detection design based on g-C3N4/TiO2 nanotubes hybrid film. Sensors Actuators B Chem 230:231–241CrossRefGoogle Scholar
  5. 5.
    Kang WJ, Ding YY, Zhou H, Liao QY, Yang X, Yang YG, Jiang JS, Yang MH (2015) Monitoring the activity and inhibition of alkaline phosphatase via quenching and restoration of the fluorescence of carbon dots. Microchim Acta 182:1161–1167CrossRefGoogle Scholar
  6. 6.
    Ximenes VF, Campa A, Baader WJ, Catalani LH (1999) Facile chemiluminescent method for alkaline phosphatase determination. Anal Chim Acta 402:99–104CrossRefGoogle Scholar
  7. 7.
    Liu SG, Han L, Li N, Xiao N, Ju YJ, Li NB, Luo HQ (2018) A fluorescence and colorimetric dual-mode assay of alkaline phosphatase activity via destroying oxidase-like CoOOH nanoflakes. J Mater Chem B 6:2843–2850CrossRefGoogle Scholar
  8. 8.
    Shen CC, Li XZ, Rasooly A, Guo LY, Zhang KN, Yang MH (2016) A single electrochemical biosensor for detecting the activity and inhibition of both protein kinase and alkaline phosphatase based on phosphate ions induced deposition of redox precipitates. Biosens Bioelectron 85:220–225CrossRefGoogle Scholar
  9. 9.
    Chen D, Zhang H, Li X, Li J (2010) Bio-functional Titania nanotubes for visible-light activated Photoelectrochemical biosensing. Anal Chem 82:2253–2261CrossRefGoogle Scholar
  10. 10.
    Chang H, Zhang H, Lv X, Li JH (2010) Quantum dots sensitized graphene: in situ growth and application in photoelectrochemical cells. Electrochem Commun 12:483–487CrossRefGoogle Scholar
  11. 11.
    Xu R, Jiang Y, Xia L, Zhang T, Xu L, Zhang S, Liu D, Song H (2015) A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs. Biosens Bioelectron 74:411–417CrossRefGoogle Scholar
  12. 12.
    Li C, Feng C, Qu F, Liu J, Zhu L, Lin Y, Wang Y, Li F, Zhou J, Ruan S (2015) Electrospun nanofibers of p-type NiO/n-type ZnO heterojunction with different NiO content and its influence on trimethylamine sensing properties. Sensors Actuators B Chem 207:90–96CrossRefGoogle Scholar
  13. 13.
    Liu Y, Li J, Wang M, Li Z, Liu H, He P, Yang X, Li J (2005) Preparation and properties of nanostructure Anatase TiO2 monolith by using 1-butyl-3-methylimidazolium tetrafluoroborate room-temperature ionic liquids as template solvent. Cryst Growth Des 5:1643–1649CrossRefGoogle Scholar
  14. 14.
    Lu X, Wen Z, Li J (2006) Hydroxyl-containing antimony oxide bromide nanorods combined with chitosan for biosensors. Biomaterials 27:5740–5747CrossRefGoogle Scholar
  15. 15.
    Dai WX, Zhang L, Zhao WW, Yu XD, Xu JJ, Chen HY (2017) Hybrid PbS quantum dot/nanoporous NiO film nanostructure: preparation, characterization, and application for a self-powered cathodic photoelectrochemical biosensor. Anal Chem 89:8070–8078CrossRefGoogle Scholar
  16. 16.
    Kong RM, Zhao Y, Zheng YQ, Qu FL (2017) Facile synthesis of ZnO/CdS@ZIF-8 core–shell nanocomposites and their applications in photocatalytic degradation of organic dyes. RSC Adv 7:31365–31371CrossRefGoogle Scholar
  17. 17.
    Zhang XP, Zhang RR, Yang AJ, Wang Q, Kong RM, Qu FL (2017) Aptamer based photoelectrochemical determination of tetracycline using a spindle-like ZnO-CdS@au nanocomposite. Microchim Acta 184:4367–4374CrossRefGoogle Scholar
  18. 18.
    Zhao Y, Gong J, Zhang XB, Kong RM, Qu FL (2018) Enhanced biosensing platform constructed using urchin-like ZnO-au@CdS microspheres based on the combination of photoelectrochemical and bioetching strategies. Sens Actuators B: Chem 255:1753–1761CrossRefGoogle Scholar
  19. 19.
    Zhao YY, Li L, Yu RQ, Chen TT, Chu X (2017) CoOOH-induced synthesis of fluorescent polydopamine nanoparticles for the detection of ascorbic acid. Anal Methods 9:5518–5524CrossRefGoogle Scholar
  20. 20.
    Cen Y, Yang Y, Yu RQ, Chen TT, Chu X (2016) A cobalt oxyhydroxide nanoflake-based nanoprobe for the sensitive fluorescence detection of T4 polynucleotide kinase activity and inhibition. Nanoscale 8:8202–8209CrossRefGoogle Scholar
  21. 21.
    Tang FM, Cheng WR, Su H, Zhao X, Liu QH (2018) Smoothing surface trapping states in 3D coral-like CoOOH wrapped-BiVO4 for efficient photoelectrochemical water oxidation. ACS Appl Mater Interfaces 10:6228–6234CrossRefGoogle Scholar
  22. 22.
    Gerken JB, McAlpin JG, Chen J, Rigsby ML, Casey WH, Britt RD, Stahl SS (2011) Electrochemical water oxidation with cobalt-based electrocatalysts from pH 0-14: the thermodynamic basis for catalyst structure, stability, and activity. J Am Chem Soc 33:14431–14442CrossRefGoogle Scholar
  23. 23.
    Wu JL, Bai S, Shen XP, Jiang L (2010) Preparation and characterization of graphene/CdS nanocomposites. Appl Surf Sci 257:747–751CrossRefGoogle Scholar
  24. 24.
    Kulkarni SB, Jamadade VS, Dhawale DS, Lokhande CD (2009) Synthesis and characterization of β-Ni(OH)2 up grown nanoflakes by SILAR method. Appl Surf Sci 255:8390–8394CrossRefGoogle Scholar
  25. 25.
    Jagadale AD, Dubal DP, Lokhand CD (2012) Electrochemical behavior of potentiodynamically deposited cobalt oxyhydroxide (CoOOH) thin films for supercapacitor application. Mater Res Bull 47:672–676CrossRefGoogle Scholar
  26. 26.
    Kandalkar SG, Dhawale DS, Kim CK, Lokhande CD (2010) Chemical synthesis of cobalt oxide thin film electrode for supercapacitor application. Synth Met 160:1299–1302CrossRefGoogle Scholar
  27. 27.
    Xu Y, Bai H, Lu G, Li C, Shi G (2008) Flexible graphene films via the filtration of water-soluble noncovalent functionalized graphene sheets. J Am Chem Soc 130:5856–5857CrossRefGoogle Scholar
  28. 28.
    Titelman GI, Gelman V, Bron S, Khalfin RL, Cohen Y, Bianco-Peled H (2005) Characteristics and microstructure of aqueous colloidal dispersions of graphite oxide. Carbon 43:641–649CrossRefGoogle Scholar
  29. 29.
    Yun JH, Wong RJ, Ng YH, Du A, Amal R (2012) Combined electrophoretic deposition-anodization method to fabricate reduced graphene oxide-TiO2 nanotube films. RSC Adv 2:8164–8171CrossRefGoogle Scholar
  30. 30.
    Kumar PN, Mishra SK, Kannan S (2017) Structural perceptions and mechanical evaluation of β-Ca3(PO4)2/c-CeO2 composites with preferential occupancy of Ce3+ and Ce4+. Inorg Chem 56:3600–3611CrossRefGoogle Scholar
  31. 31.
    Hu T, Li P, Zhang J, Liang C, Dai K (2018) Highly efficient direct Z-scheme WO3/CdS-diethylenetriamine photocatalyst and its enhanced photocatalytic H2 evolution under visible light irradiation. Appl Surf Sci 442:20–29CrossRefGoogle Scholar
  32. 32.
    Yu J, Jin J, Cheng B, Jaroniec M (2014) A noble metal-free reduced graphene oxide-CdS nanorod composite for the enhanced visible-light photocatalytic reduction of CO2 to solar fuel. J Mater Chem A 2:3407–3416CrossRefGoogle Scholar
  33. 33.
    Lv J, Zhang J, Dai K, Liang C, Zhu G, Wang Z, Li Z (2017) Controllable synthesis of inorganic-organic Zn1-xCdxS-DETA solid solution nanoflowers and their enhanced visible-light photocatalytic hydrogen-production performance. Dalton Trans 46:11335–11343CrossRefGoogle Scholar
  34. 34.
    Wu Q, Gao X, Li G, Pan G, Yan T, Zhu H (2007) Microstructure and electrochemical properties of Al-substituted nickel hydroxides modified with CoOOH nanoparticles. J Phys Chem C 111:17082–17087CrossRefGoogle Scholar
  35. 35.
    Casella IG, Guascito MR (1999) Anodic electrodeposition of conducting cobalt oxyhydroxide films on a gold surface. XPS study and electrochemical behaviour in neutral and alkaline solution. J Electroanal Chem 476:54–63CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringQufu Normal UniversityQufuChina
  2. 2.School of Geography and TourismQufu Normal UniversityRizhaoChina

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