Microchimica Acta

, 186:71 | Cite as

Hybrid synthetic receptor composed of molecularly imprinted polydopamine and aptamers for impedimetric biosensing of urea

  • Saeed Yarahmadi
  • Azadeh AzadbakhtEmail author
  • Reza Mir Derikvand
Original Paper


An electrochemical aptamer-based method is described for highly specific sensing of urea. Urea-imprinted polydopamine was obtained by electropolymerization of dopamine (DA). The molecularly imprinted polymer (MIP) also contains DNA aptamers on gold nanoparticles decorated with a carbon nanotube network (AuNP/CNT). The material was placed on a glassy carbon electrode (GCE). After removal of urea from the MIP cavities, the GCE display double recognition capability which makes it superior to conventional MIP-only or aptamer-only based assays. On exposure of the modified electrode to urea, the interfacial charge transfer of the redox probe hexacyanoferrate is traced, typically measured at a peak voltage of 0.22 V vs. Ag/AgCl. The change in charge transfer resistance depends on the urea concentration. The assay has a 900 fM detection limit, and response is the linear up to 500 nM urea concentrations.

Graphical abstract


Dual recognition element Molecularly imprinted polydopamine Urea Nanohybrid receptor 



The authors gratefully acknowledge the support of this work by the Khorramabad Branch, Islamic Azad University for financial support.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3180_MOESM1_ESM.docx (484 kb)
ESM 1 (DOCX 484 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Saeed Yarahmadi
    • 1
  • Azadeh Azadbakht
    • 1
    Email author
  • Reza Mir Derikvand
    • 2
  1. 1.Department of Chemistry, Khorramabad BranchIslamic Azad UniversityKhorramabadIran
  2. 2.Department of Plant Breeding, Khorramabad BranchIslamic Azad UniversityKhorramabadIran

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