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Microchimica Acta

, 186:128 | Cite as

Enantioseparation of propranolol, amlodipine and metoprolol by electrochromatography using an open tubular capillary modified with β-cyclodextrin and poly(glycidyl methacrylate) nanoparticles

  • Xiaodong Sun
  • Yingxiang DuEmail author
  • Shiyuan Zhao
  • Zhifeng Huang
  • Zijie Feng
Original Paper
  • 46 Downloads

Abstract

The inner wall of a capillary was coated with glycidyl methacrylate (GMA) to form tentacle-type coating, and poly(glycidyl methacrylate) nanoparticles (PGMA NPs) were then immobilized on the film. Ethanediamine-β-cyclodextrin as chiral selector was covalently bonded into the PGMA NPs through the ring-open reaction. The materials were characterized by SEM, TEM and FT-IR. The modified column was applied to the enantioseparation of the racemates of propranolol, amlodipine and metoprolol. Compared to a capillary with a single layer of CD-PGMA (without GMA coating) and to a CD-GMA system (without PGMA nanoparticles), the performance of the capillary is strongly improved. The effects of buffer pH value and applied voltage were optimized. Best resolutions (propranolol: 1.27, metoprolol: 1.01 and amlodipine: 2.93) were obtained when using the PGMA-coated capillary system. The run-to-run, day-to-day and column-to-column reproducibility were tested and found to be highly attractive. The new stationary phase is likely to have a large potential and scope in that it may also be applied to chiral separations of other enantiomers, such as amino acids and biogenic amines.

Graphical abstract

Schematic presentation of the preparation of a capillary column with glycidyl methacrylate (GMA) coating which was then immobilized with poly(glycidyl methacrylate) nanoparticles and ethanediamine-β-cyclodextrin. This novel open tubular column was applied to construct capillary electrochromatography system for separation of basic racemic drugs.

Keywords

Capillary electrochromatography Open-tubular capillary Poly(glycidyl methacrylate) nanoparticles Ethanediamine-β-cyclodextrin Tentacle-type coating Basic drugs Chiral separation Novel method 

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.: BK20141353) and the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3163_MOESM1_ESM.doc (1.3 mb)
ESM 1 (DOC 1298 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Xiaodong Sun
    • 1
  • Yingxiang Du
    • 1
    • 2
    • 3
    Email author
  • Shiyuan Zhao
    • 1
  • Zhifeng Huang
    • 1
  • Zijie Feng
    • 1
  1. 1.Department of Analytical ChemistryChina Pharmaceutical UniversityNanjingPeople’s Republic of China
  2. 2.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education)China Pharmaceutical UniversityNanjingPeople’s Republic of China
  3. 3.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingPeople’s Republic of China

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