A “turn-on” fluorometric assay for kanamycin detection by using silver nanoclusters and surface plasmon enhanced energy transfer
A rapid method is described for the determination of the antibiotic kanamycin. It integrates a kanamycin-binding aptamer and surface plasmon enhanced energy transfer (SPEET) between DNA-templated silver nanoclusters (AgNCs) and gold nanoparticles (AuNPs). The AgNCs and AuNPs were selected as energy donor and energy acceptor, respectively. The aptamer was designed to regulate the energy transfer between AgNCs and AuNPs. The aptamer was adsorbed on the AuNPs. Upon addition of kanamycin, the aptamer-kanamycin complex is formed, and this results in the aggregation of the AuNPs in high salt concentration, the formation of a blue coloration, and in the suppression of the SPEET process. The fluorescence of the AgNCs (with excitation/emission peaks at 560/600 nm) is quenched by the aptamer protected AuNPs in absence of kanamycin. The fluorescence on addition of kanamycin increases linearly in the 5 to 50 nM concentration range, with a lower detection limit of 1.0 nM (at S/N = 3). The assay can be performed within 30 min. It was successfully applied to the determination of kanamycin in spiked milk samples, and recoveries ranged between 90.2 and 95.4%. Conceivably, the strategy has a wide potential for screening by simply changing the aptamer.
KeywordsAptasensor Ag NCs Au NPs Antibiotics detection Food safety Milk analysis
This work was financially supported by the National Natural Science Foundation of China (31801636), Shanghai Sailing Program (Grant No. 18YF1417300), and Cultivation Science Foundation of University of Shanghai for Science and Technology (ZR17PY08).
Compliance with ethical standards
This chapter does not contain any studies with human participants or animals performed by any of the authors.
- 1.Yu YJ, Wu HL, Fu HY, Zhao J, Li YN, Li SF, Kang C, Yu RQ (2013) Chromatographic background drift correction coupled with parallel factor analysis to resolve coelution problems in three-dimensional chromatographic data: quantification of eleven antibiotics in tap water samples by high-performance liquid chromatography coupled with a diode array detector. J Chromatogr A 1302:72–80. https://doi.org/10.1016/j.chroma.2013.06.009 CrossRefPubMedGoogle Scholar
- 2.Gbylik-Sikorska M, Posyniak A, Sniegocki T, Zmudzki J (2015) Liquid chromatography-tandem mass spectrometry multiclass method for the determination of antibiotics residues in water samples from water supply systems in food-producing animal farms. Chemosphere 119:8–15. https://doi.org/10.1016/j.chemosphere.2014.04.105 CrossRefPubMedGoogle Scholar
- 9.Tang Y, Gu C, Wang C, Song B, Zhou X, Lou X, He M (2018) Evanescent wave aptasensor for continuous and online aminoglycoside antibiotics detection based on target binding facilitated fluorescence quenching. Biosens Bioelectron 102:646–651. https://doi.org/10.1016/j.bios.2017.12.006 CrossRefPubMedGoogle Scholar
- 15.Liu X, Wang F, Aizen R, Yehezkeli O, Willner I (2013) Graphene oxide/nucleic-acid-stabilized silver nanoclusters: functional hybrid materials for optical aptamer sensing and multiplexed analysis of pathogenic DNAs. J Am Chem Soc 135(32):11832–11839. https://doi.org/10.1021/ja403485r CrossRefPubMedGoogle Scholar
- 19.Zhang K, Wang K, Zhu X, Xie M (2015) A new signal-on method for the detection of protein based on binding-induced strategy and photoinduced electron transfer between ag nanoclusters and split G-quadruplex-hemin complexes. Anal Chim Acta 887:224–229. https://doi.org/10.1016/j.aca.2015.07.015 CrossRefPubMedGoogle Scholar
- 21.Chen CW, Wang CH, Wei CM, Hsieh CY, Chen YT, Chen YF, Lai CW, Liu CL, Hsieh CC, Chou PT (2010) Highly sensitive emission sensor based on surface Plasmon enhanced energy transfer between gold nanoclusters and silver nanoparticles. J Phys Chem C 114(2):799–802. https://doi.org/10.1021/jp908387y CrossRefGoogle Scholar
- 27.Ramezani M, Danesh NM, Lavaee P, Abnous K, Taghdisi SM (2016) A selective and sensitive fluorescent aptasensor for detection of kanamycin based on catalytic recycling activity of exonuclease III and gold nanoparticles. Sens Actuators B Chem 222:1–7. https://doi.org/10.1016/j.snb.2015.08.024 CrossRefGoogle Scholar
- 31.Wang YS, Ma TC, Ma SY, Liu YJ, Tian YP, Wang RN, Jiang YB, Hou DJ, Wang JL (2017) Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots. Microchim Acta 184(1):203–210. https://doi.org/10.1007/s00604-016-2011-4 CrossRefGoogle Scholar
- 32.Lai C, Liu XG, Qin L, Zhang C, Zeng GM, Huang DL, Cheng M, Xu P, Yi H, Huang DW (2017) Chitosan-wrapped gold nanoparticles for hydrogen-bonding recognition and colorimetric determination of the antibiotic kanamycin. Microchim Acta 184(7):2097–2105. https://doi.org/10.1007/s00604-017-2218-z CrossRefGoogle Scholar
- 35.Dehghani S, Danesh NM, Ramezani M, Alibolandi M, Lavaee P, Nejabat M, Abnous K, Taghdisi SM (2018) A label-free fluorescent aptasensor for detection of kanamycin based on dsDNA-capped mesoporous silica nanoparticles and rhodamine B. Anal Chim Acta 1030:142–147. https://doi.org/10.1016/j.aca.2018.05.003 CrossRefPubMedGoogle Scholar
- 36.Zhao M, Zhuo Y, Chai Y-Q, Yuan R (2015) Au nanoparticles decorated C60 nanoparticle-based label-free electrochemiluminesence aptasensor via a novel “on-off-on” switch system. Biomaterials 52:476–483. https://doi.org/10.1016/j.biomaterials.2015.02.058 CrossRefPubMedGoogle Scholar