Microchimica Acta

, 186:61 | Cite as

Superlattice stacking by hybridizing layered double hydroxide nanosheets with layers of reduced graphene oxide for electrochemical simultaneous determination of dopamine, uric acid and ascorbic acid

  • Muhammad Asif
  • Ayesha Aziz
  • Haitao Wang
  • Zhengyun Wang
  • Wei Wang
  • Muhammad Ajmal
  • Fei XiaoEmail author
  • Xuedong ChenEmail author
  • Hongfang LiuEmail author
Original Paper


A self-assembled periodic superlattice material was obtained by integrating positively charged semiconductive sheets of a Zn-NiAl layered double hydroxide (LDH) and negatively charged layers of reduced graphene oxide (rGO). The material was used to modify a glassy carbon electrode which then is shown to be a viable sensor for the diagnostic parameters dopamine (DA), uric acid (UA) and ascorbic acid (AA). The modified GCE displays excellent electrocatalytic activity towards these biomolecules. This is assumed to be due to the synergistic effects of (a) excellent interfacial electrical conductivity that is imparted by direct neighboring of conductive rGO to semiconductive channels of LDHs, (b) the superb intercalation feature of LDHs, and (c) the enlarged surface with an enormous number of active sites. The biosensor revealed outstanding electrochemical performances in terms of selectivity, sensitivity, and wide linear ranges. Typically operated at working potentials of −0.10, +0.13 and + 0.27 V vs. saturated calomel electrode, the lower detection limits for AA, DA and UA are 13.5 nM, 0.1 nM, and 0.9 nM, respectively, at a signal-to-noise ratio of 3. The sensor was applied to real-time tracking of dopamine efflux from live human nerve cells.

Graphical abstract

Schematic of the preparation of a superlattice self-assembled material by integrating positively charged semiconductive sheets of Zn-NiAl layered double hydroxide (LDH) with negatively charged reduced graphene oxide (rGO) layers. It was applied to simultaneous electrochemical detection of dopamine (DA), uric acid and ascorbic acid.


Direct neighboring Controllable co-feeding protocol Zn-NiAl LDH/rGO Cyclic voltammetry Differential pulse voltammetry Live cells Urine sample 



National Natural Science Foundation of China (No. 51572094, 51435006), the Innovation Foundation of Huazhong University of Science (No. 2015TS150, 2015ZZGH010) and Technology and the Key Laboratory for Large-Format Battery Materials and System. We acknowledge the support of the Analytical and Testing Center of the Huazhong University of Science and Technology for SEM and XPS measurements.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3158_MOESM1_ESM.doc (668 kb)
ESM 1 (DOC 667 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  2. 2.State Key Laboratory of Digital Manufacturing Equipment and TechnologyHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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