Microchimica Acta

, 186:1 | Cite as

Voltammetric aptasensor for sulfadimethoxine using a nanohybrid composed of multifunctional fullerene, reduced graphene oxide and Pt@Au nanoparticles, and based on direct electron transfer to the active site of glucose oxidase

  • Huan You
  • Zhaode Mu
  • Min Zhao
  • Jing Zhou
  • Yongjie Chen
  • Lijuan BaiEmail author
Original Paper


This work describes a voltammetric and ultrasensitive aptasensor for sulfadimethoxine (SDM). It is based on signal amplification by making use of a multifunctional fullerene-doped reduced graphene oxide nanohybrid. The nanohybrid was coated with poly(diallyldimethylammonium chloride) to obtain a material (P-C60-rGO) with large specific surface area and a unique adsorption ability for loading it with glucose oxidase (GOx). The coating also facilitates the direct electron transfer between the active site of GOx and the glassy carbon electrode (GCE). The P-C60-rGO were then modified with Pt@Au nanoparticles, and the thiolated SDM-binding aptamer was immobilized on the nanoparticles. On exposure of the modified GCE to a solution containing SDM, it binds to the aptamer. The results were recorded through the signal responses generated from the redox center of GOx (FAD/FADH2) by cyclic voltammetry at a scan rate of 100 mV·s−1 from −0.25 to −0.65 V. Accordingly, The sensor has good specificity and stability, and response is linear in the 10 fg·mL−1 to 50 ng·mL−1 SDM concentration range with a detection limit of 8.7 fg·mL−1.

Graphical abstract

Schematic presentation of an electrochemical aptasensor for sulfadimethoxine (SDM) using multifunctional fullerene-doped graphene (C60-rGO) nanohybrids for amplification. The limit of detection for SDM is as low as 8.7 fg·mL−1.


Poly(diallyldimethylammonium chloride) Aptamer Electrochemical detection Glucose oxidase 



This work is supported by National Natural Science Foundation of China (81601856), the Third Batch of Young Backbone Teachers Funding Program in Colleges and Universities of Chongqing City ([2016] No. 53) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City (cstc2014kjrc-qnrc00004).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3127_MOESM1_ESM.doc (986 kb)
ESM 1 (DOC 986 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Huan You
    • 1
  • Zhaode Mu
    • 1
  • Min Zhao
    • 2
  • Jing Zhou
    • 1
  • Yongjie Chen
    • 1
  • Lijuan Bai
    • 1
    Email author
  1. 1.Engineering Technology Research Center for Pharmacodynamic Evaluation of Chongqing, College of PharmacyChongqing Medical UniversityChongqingPeople’s Republic of China
  2. 2.Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory MedicineChongqing Medical UniversityChongqingPeople’s Republic of China

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