Voltammetric aptasensor for sulfadimethoxine using a nanohybrid composed of multifunctional fullerene, reduced graphene oxide and Pt@Au nanoparticles, and based on direct electron transfer to the active site of glucose oxidase
This work describes a voltammetric and ultrasensitive aptasensor for sulfadimethoxine (SDM). It is based on signal amplification by making use of a multifunctional fullerene-doped reduced graphene oxide nanohybrid. The nanohybrid was coated with poly(diallyldimethylammonium chloride) to obtain a material (P-C60-rGO) with large specific surface area and a unique adsorption ability for loading it with glucose oxidase (GOx). The coating also facilitates the direct electron transfer between the active site of GOx and the glassy carbon electrode (GCE). The P-C60-rGO were then modified with Pt@Au nanoparticles, and the thiolated SDM-binding aptamer was immobilized on the nanoparticles. On exposure of the modified GCE to a solution containing SDM, it binds to the aptamer. The results were recorded through the signal responses generated from the redox center of GOx (FAD/FADH2) by cyclic voltammetry at a scan rate of 100 mV·s−1 from −0.25 to −0.65 V. Accordingly, The sensor has good specificity and stability, and response is linear in the 10 fg·mL−1 to 50 ng·mL−1 SDM concentration range with a detection limit of 8.7 fg·mL−1.
KeywordsPoly(diallyldimethylammonium chloride) Aptamer Electrochemical detection Glucose oxidase
This work is supported by National Natural Science Foundation of China (81601856), the Third Batch of Young Backbone Teachers Funding Program in Colleges and Universities of Chongqing City ( No. 53) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City (cstc2014kjrc-qnrc00004).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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