Microchimica Acta

, 186:53 | Cite as

In-situ growth of a metal organic framework composed of zinc(II), adeninate and biphenyldicarboxylate as a stationary phase for open-tubular capillary electrochromatography

  • Zhentao Li
  • Zhenkun Mao
  • Zilin ChenEmail author
Original Paper


This work reports on the in-situ growth of a metal organic framework (MOF) composed of zinc(II), adeninate and biphenyldicarboxylate on the inner wall of a capillary, and the use of this MOF as a stationary phases in open-tubular capillary electrochromatography. The inner wall of a fused-silica capillary was first modified with 3-aminopropyltriethoxysilane to create surface amino sites, and then the MOF was synthesized by in-situ growth. The modified capillary was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. The results proved the successful growth of the MOF. The resultant open-tubular column showed good separation selectivity towards neutral compounds, acidic and basic compounds including nonsteroidal anti-inflammatory drugs, sulfa drugs and small biomolecules. In addition, the modified column is stable and repeatable. The precisions (expressed as RSDs) of the retention time for intra-day (n = 5) and inter-day (n = 5) separations and between columns (n = 3) are less than 0.5, 1.6 and 4.7%, respectively. Conceivably, this new kind of MOF represents a most useful novel stationary phase in electrochromatographic separations.

Graphical abstract

Schematic presentation of the open-tubular column modified with bio-MOF-1 by in situ hydrothermal reaction with adeninate, Zn(II) and biphenyldicarboxylate for capillary electrochromatographic separations.


In situ hydrothermal reaction Bio-MOF-1 Open tubular column Drug analysis Nonsteroidal anti-inflammatory drugs Sulfa drugs Biomolecules Electrochromatographic separation 



This work was supported by the National Natural Science Foundation of China (Grant Nos. 81872828 and 81573384).

Supplementary material

604_2018_3115_MOESM1_ESM.docx (4.5 mb)
ESM 1 (DOCX 4.50 MB)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical SciencesWuhanChina
  2. 2.State Key Laboratory of Transducer Technology, Chinese Academy of SciencesBeijingChina

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