Microchimica Acta

, 186:13 | Cite as

Colorimetric determination of glucose in solution and via the use of a paper strip by exploiting the peroxidase and oxidase mimicking activity of bimetallic Cu-Pd nanoparticles deposited on reduced graphene oxide, graphitic carbon nitride, or MoS2 nanosheets

  • Gitashree Darabdhara
  • Purna K. Boruah
  • Manash R. DasEmail author
Original Paper


This work describes the preparation of bimetallic Cu-Pd nanoparticles (NPs) on supports like reduced graphene oxide (rGO), graphitic carbon nitride (g-C3N4) and MoS2 sheets with a size of <10 nm. rGO is found to be the best support for synthesizing Cu-Pd NPs with controlled shape, size and oxidation state. The Cu-Pd/rGO nanocomposite also demonstrated the best peroxidase and oxidase mimicking activity compared to Cu-Pd/g-C3N4 and Cu-Pd/MoS2 nanocomposites. The peroxidase mimicking activity of Cu-Pd/rGO was investigated in more detail, and a glucose oxidase (GOx) based glucose sensor was constructed that is based on the enzymatic formation of H2O2 and the Cu-Pd NPs-assisted oxidation of tetramethylbenzidine by H2O2 to give a blue-green coloration with absorption maxima at 652 nm. The assay has a 0.29 μM detection limit and a detection range that extends from 0.2 to 50 μM. The method was applied to the determination of glucose in diluted serum samples, and results compared well to those acquired with a clinical analyzer. The method also was applied in a colorimetric paper-based test stripe that can detect glucose within 10 min.

Graphical abstract

Schematic representation of a sensitive colorimetric glucose assay based on bimetallic Cu-Pd nanoparticles supported on 2D nanosheets, and construction of a paper based device for rapid glucose detection.


2D nanosheets 3,3′,5,5′-Tetramethylbenzidine Colorimetric assay Glucose assay Paper device Enzyme mimic 



Authors thank the Director, CSIR−NEIST, Jorhat. GD acknowledges DST, India for DST-INSPIRE Fellowship (GAP–0726). The authors acknowledges clinical center, CSIR-NEIST for providing the serum samples.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3112_MOESM1_ESM.docx (4.9 mb)
ESM 1 (DOCX 4.85 mb)


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Copyright information

© Springer-Verlag GmbH Austria, ein Teil von Springer Nature 2018

Authors and Affiliations

  1. 1.Advanced Materials Group, Materials Sciences and Technology DivisionCSIR-North East Institute of Science and TechnologyJorhatIndia
  2. 2.Academy of Scientific and Innovative ResearchCSIR - Human Resource Development CentreGhaziabadIndia

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