A voltammetric assay for microRNA-25 based on the use of amino-functionalized graphene quantum dots and ss- and ds-DNAs as gene probes
- 138 Downloads
The authors describe a DNA based voltammetric assay for the cancer biomarker microRNA-25. A glassy carbon electrode (GCE) was modified with amino-functionalized graphene quantum dots and used as an amplifier of electrochemical signals. p-Biphenol is introduced as a new electroactive probe with a fairly low working potential of 0.3 V (vs. Ag/AgCl). The stages of fabricating the electrode were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. ss-Probe DNA was immobilized on the modified GCE and then exposed to a sample containing microRNA-25. The results indicated that the electrode can distinguish complementary microRNA-25 from a single-base mismatch. The increase in the electrochemical response of PBP and the positive shift in the potential peak indicate that PBP is intercalated between two strands. Under optimized experimental conditions, the current of the electrode increases linearly with the logarithm of the microRNA-25 concentration in the range from 0.3 nM to 1.0 μM, and the detection limit is 95.0 pM. The assay was successfully employed to the determination of microRNA-25 in spiked human plasma.
KeywordsPyrolysis Citric acid p-Biphenol Amino-functionalized graphene quantum dots microRNA-25 Electroactive label
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 3.Tüfekci KU, Öner MG, Meuwissen RLJ, Genç Ş (2014) The role of microRNAs in human diseases. miRNomics: MicroRNA biology and computational analysis. In: Methods in Molecular Biology (Methods and Protocols), vol 1107. Humana Press, TotowaGoogle Scholar
- 5.Liu L, Jiang S, Wang L, Zhang Z, Xie G (2015) Direct detection of microRNA-126 at a femtomolar level using a glassy carbon electrode modified with chitosan, graphene sheets, and a poly (amidoamine) dendrimer composite with gold and silver nanoclusters. Microchim Acta 182:77–846CrossRefGoogle Scholar
- 12.Xia N, Wang X, Deng D, Wang G, Zhai H, Li S-J (2013) Label-free electrochemical sensor for MicroRNAs detection with ferroceneboronic acids as redox probes. Int J Electrochem Sci 8:9714–9722Google Scholar
- 15.Belsare PU, Zade AB (2013) Synthesis, characterization and thermal study of 2, 2′-biphenol-tetraethylenepentamine-formaldehyde terpolymer resin. Der Pharma Chemica 5(4):325–334Google Scholar
- 18.Laurenti M, Paez-Perez M, Algarra M, Alonso-Cristobal P, Lopez-Cabarcos E, Mendez-Gonzalez D, Rubio-Retama J (2016) Enhancement of the upconversion emission by visible-to-near-infrared fluorescent graphene quantum dots for miRNA detection. ACS Appl Mater Interfaces 8:12644–12651CrossRefGoogle Scholar
- 27.Miller JN, Miller JC (2000) Statistics and Chemometrics for analytical chemistry, 4th Pearson education limited, EssexGoogle Scholar