Aptamer based voltammetric patulin assay based on the use of ZnO nanorods
The authors describe an aptamer based assay for the mycotoxin patulin (PAT). A gold electrode was modified with a composite made from ZnO nanorods (ZnO-NRs) and chitosan. The ZnO-NRs was prepared by reaction with ammonia and subsequent hydrothermal growth. Its properties were characterized by X-ray diffraction, Raman spectroscopy and scanning electron microscopy. Subsequently, thiol-modified aptamers were self-assembled on AuNPs that had been electrodeposited on the surface of the modified electrode. The presence of ZnO-NRs on the electrode increases the loading with AuNPs and aptamers. It also warrants a relatively stable microenvironment for the aptamers. In the presence of PAT, it will form a complex with the aptamer on the electrode surface. This hinders electron transfer from the electrode to the redox probe hexacyanoferrate and results in reduced current, which is typically measured at 0.176 V (vs. Ag/AgCl). The concentration of PAT can be calculated from the differences in the peak current before and after incubation with PAT. The assay has a linear response in the 50 ng·mL−1 to 0.5 pg·mL−1 PAT concentration range and a 0.27 pg·mL−1 lower detection limit. The sensor is specific, reproducible, repeatable, and long-term stable. It was successfully applied to the determination of PAT in spiked juice samples.
KeywordsAptasensor Patulin Chitosan Electrodeposited gold nanoparticles Rapid detection
This work was supported by the National Natural Science Foundation of China (Grant No. 61301037), the Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), the Henan Science and Technology Cooperation Project (Grant No. 172106000014), and the Youth Backbone Teacher Training Program of Henan University of Technology.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 3.Yuan XU, Jiang LP, Chen M, Geng CY, Wei YW, Yang G, Zhang LF, Liu XF (2014) The lysosomal pathway of dna damage induced by patulin in hek-293 cells. J Toxicol 28(3):203–207Google Scholar
- 4.dos Santos ID, Pizzutti IR, Dias JV, Fontana MEZ, Brackmann A, Anese RO, Thewes FR, Marques LN, Cardoso CD (2018) Patulin accumulation in apples under dynamic controlled atmosphere storage. Food Chem 255:275–281Google Scholar
- 6.Liu BJ, Peng XN, Meng XH (2018) Effective biodegradation of mycotoxin patulin by porcine pancreatic lipase. Front Microbiol 9(615):1–7Google Scholar
- 32.Duan N, Zhang WX, Wu SJ, Wang ZP (2016) A colorimetric method for Patulin detection based on aptamer and gold nanoparticles. Sci Sinica Chim 46(3):268–273Google Scholar