Advertisement

Microchimica Acta

, 185:457 | Cite as

A catalytic cleavage strategy for fluorometric determination of Hg(II) based on the use of a Mg(II)-dependent split DNAzyme and hairpins conjugated to gold nanoparticles

  • Wen Yun
  • Fukun Li
  • Xingyan Liu
  • Ning Li
  • Lin Chen
  • Lizhu Yang
Original Paper
First Online:
Received:
Accepted:
  • 37 Downloads

Abstract

A catalytic cleavage strategy was developed for the fluorometric determination of Hg(II). The method is based on the use of a Mg(II)-dependent split DNAzyme. Fluorophore labeled hairpins were conjugated to gold nanoparticles (AuNPs) upon which fluorescence is quenched. Thymine-Hg(II)-thymine (T-Hg(II)-T) interaction causes the two DNA sequences to form an entire enzyme-strand DNA (E-DNA). The E-DNA bind to the hairpins on the AuNPs to form a Mg(II)-dependent DNAzyme structure. The circular cleavage of hairpins results in a signal amplification and in the recovery of fluorescence. The assay has a limit of detection (LOD) as low as 80 pM of Hg(II). This LOD is comparable to those obtained with other amplification strategies. The method was successfully applied to the determination of Hg(II) in Chinese herbs (Atractylodes macrocephala Koidz).

Graphical abstract

Schematic of a catalytic cleavage strategy based on Mg(II)-dependent split DNAzyme for fluorometric determination of Hg(II).

Keywords

AuNPs Split DNAzyme Amplification Enzyme-free detection T-Hg(II)-T 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgements

This work is sponsored by Chongqing Key Laboratory of Catalysis and New Environmental Materials (Grant No. KFJJ2017033), Open Research Fund (CQCM-2016-05), the Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences (Grant No.201712), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJ1706156), the Project of Wenzhou Science &Technology Bureau (W20170006), the Open Project of State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials (Grant No. 17kffk06), the Startup Foundation of Chongqing Technology and Business University (Grant No.1756001), National Natural Science Foundation of China (Grant No. 21601164; 31300819).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2990_MOESM1_ESM.doc (798 kb)
ESM 1 (DOC 797 kb)

References

  1. 1.
    Yun W, Xiong W, Wu H, Fu M, Huang Y, Liu X, Yang L (2017) Graphene oxide-based fluorescent “turn-on” strategy for Hg2+ detection by using catalytic hairpin assembly for amplification. Sensor Actuat B-Chem 249:493–498CrossRefGoogle Scholar
  2. 2.
    Zhao Y, Qiang H, Chen Z (2017) Colorimetric determination of hg (II) based on a visually detectable signal amplification induced by a cu@ au-hg trimetallic amalgam with peroxidase-like activity. Microchim Acta 184(1):107–115CrossRefGoogle Scholar
  3. 3.
    Gorski PR, Cleckner LB, Hurley JP, Sierszen ME, Armstrong DE (2003) Factors affecting enhanced mercury bioaccumulation in inland lakes of isle Royale National Park, USA. Sci Total Environ 304(1):327–348CrossRefPubMedGoogle Scholar
  4. 4.
    Koneswaran M, Narayanaswamy R (2016) Retraction note to: CdS/ZnS core-shell quantum dots capped with mercaptoacetic acid as fluorescent probes for hg (II) ions. Microchim Acta 183(4):1519–1519CrossRefGoogle Scholar
  5. 5.
    Zhang L, Li T, Li B, Li J, Wang E (2010) Carbon nanotube–DNA hybrid fluorescent sensor for sensitive and selective detection of mercury (II) ion. Chem Commun 46(9):1476–1478CrossRefGoogle Scholar
  6. 6.
    Jarzyńska G, Falandysz J (2011) The determination of mercury in mushrooms by CV-AAS and ICP-AES techniques. J Environ Sci Heal A 46(6):569–573CrossRefGoogle Scholar
  7. 7.
    Jackson B, Taylor V, Baker RA, Miller E (2009) Low-level mercury speciation in freshwaters by isotope dilution GC-ICP-MS. Environ Sci Technol 43(7):2463–2469CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Liang L-N, Jiang G-B, Liu J-F, Hu J-T (2003) Speciation analysis of mercury in seafood by using high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry via a post column microwave digestion. Anal Chim Acta 477(1):131–137CrossRefGoogle Scholar
  9. 9.
    Miyake Y, Togashi H, Tashiro M, Yamaguchi H, Oda S, Kudo M, Tanaka Y, Kondo Y, Sawa R, Fujimoto T (2006) MercuryII-mediated formation of thymine- HgII-thymine base pairs in DNA duplexes. J Am Chem Soc 128(7):2172–2173CrossRefPubMedGoogle Scholar
  10. 10.
    Yang L, Yun W, Chen Y, Wu H, Liu X, Fu M, Huang Y (2017) Ultrasensitive colorimetric and fluorometric detection of hg (II) based on the use of gold nanoparticles and a catalytic hairpin assembly. Microchim Acta 184(12):4741–4747CrossRefGoogle Scholar
  11. 11.
    Han D, Lim SY, Kim BJ, Piao L, Chung TD (2010) Mercury (II) detection by SERS based on a single gold microshell. Chem Commun 46(30):5587–5589CrossRefGoogle Scholar
  12. 12.
    Zhang H, Fu X, Deng X, Wang Q, Gu D (2017) Hg2+-triggered exonuclease III-assisted dual-cycle targets recycling amplification for label-free and ultrasensitive colorimetric detection of Hg2+. Sensor Actuat B-Chem 246:896–903CrossRefGoogle Scholar
  13. 13.
    Zhang Z, Fu X, Li K, Liu R, Peng D, He L, Wang M, Zhang H, Zhou L (2016) One-step fabrication of electrochemical biosensor based on DNA-modified three-dimensional reduced graphene oxide and chitosan nanocomposite for highly sensitive detection of hg (II). Sensor Actuat B-Chem 225:453–462CrossRefGoogle Scholar
  14. 14.
    Zhang M, Ge L, Ge S, Yan M, Yu J, Huang J, Liu S (2013) Three-dimensional paper-based electrochemiluminescence device for simultaneous detection of Pb2+ and Hg2+ based on potential-control technique. Biosens Bioelectron 41:544–550CrossRefPubMedGoogle Scholar
  15. 15.
    Yuan M, Zhu Y, Lou X, Chen C, Wei G, Lan M, Zhao J (2012) Sensitive label-free oligonucleotide-based microfluidic detection of mercury (II) ion by using exonuclease I. Biosens Bioelectron 31(1):330–336CrossRefPubMedGoogle Scholar
  16. 16.
    Zhu X, Zhou X, Xing D (2011) Ultrasensitive and selective detection of mercury (II) in aqueous solution by polymerase assisted fluorescence amplification. Biosens Bioelectron 26(5):2666–2669CrossRefPubMedGoogle Scholar
  17. 17.
    Ren W, Zhang Y, Huang WT, Li NB, Luo HQ (2015) Label-free colorimetric detection of Hg2+ based on Hg2+-triggered exonuclease III-assisted target recycling and DNAzyme amplification. Biosens Bioelectron 68:266–271CrossRefPubMedGoogle Scholar
  18. 18.
    Li D, Wieckowska A, Willner I (2008) Optical analysis of Hg2+ ions by oligonucleotide–gold-nanoparticle hybrids and DNA-based machines. Angew Chem Int Ed 47(21):3927–3931CrossRefGoogle Scholar
  19. 19.
    Huang J, Gao X, Jia J, Kim J-K, Li Z (2014) Graphene oxide-based amplified fluorescent biosensor for Hg2+ detection through hybridization chain reactions. Anal Chem 86(6):3209–3215CrossRefPubMedGoogle Scholar
  20. 20.
    Yuan Y, Gao M, Liu G, Chai Y, Wei S, Yuan R (2014) Sensitive pseudobienzyme electrocatalytic DNA biosensor for mercury (II) ion by using the autonomously assembled hemin/G-quadruplex DNAzyme nanowires for signal amplification. Anal Chim Acta 811:23–28CrossRefPubMedGoogle Scholar
  21. 21.
    Yun W, Li H, Chen S, Tu D, Xie W, Huang Y (2014) Aptamer-based rapid visual biosensing of melamine in whole milk. Eur Food Res Technol 238(6):989–995CrossRefGoogle Scholar
  22. 22.
    Demers LM, Mirkin CA, Mucic RC, Reynolds RA 3rd, Letsinger RL, Elghanian R, Viswanadham G (2000) A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Anal Chem 72(22):5535–5541CrossRefPubMedGoogle Scholar
  23. 23.
    Qi L, Zhao Y, Yuan H, Bai K, Zhao Y, Chen F, Dong Y, Wu Y (2012) Amplified fluorescence detection of mercury (II) ions (Hg2+) using target-induced DNAzyme cascade with catalytic and molecular beacons. Analyst 137(12):2799–2805CrossRefPubMedGoogle Scholar
  24. 24.
    Tan D, He Y, Xing X, Zhao Y, Tang H, Pang D (2013) Aptamer functionalized gold nanoparticles based fluorescent probe for the detection of mercury (II) ion in aqueous solution. Talanta 113:26–30CrossRefPubMedGoogle Scholar
  25. 25.
    Zhu Y, Cai Y, Zhu Y, Zheng L, Ding J, Quan Y, Wang L, Qi B (2015) Highly sensitive colorimetric sensor for Hg2+ detection based on cationic polymer/DNA interaction. Biosens Bioelectron 69:174–178CrossRefPubMedGoogle Scholar
  26. 26.
    Deng L, Zhou Z, Li J, Li T, Dong S (2011) Fluorescent silver nanoclusters in hybridized DNA duplexes for the turn-on detection of Hg2+ ions. Chem Commun 47(39):11065–11067CrossRefGoogle Scholar
  27. 27.
    Zhang Y, Yuan Q, Chen T, Zhang X, Chen Y, Tan W (2012) DNA-capped mesoporous silica nanoparticles as an ion-responsive release system to determine the presence of mercury in aqueous solutions. Anal Chem 84(4):1956–1962CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Li Q, Zhou X, Xing D (2010) Rapid and highly sensitive detection of mercury ion (Hg2+) by magnetic beads-based electrochemiluminescence assay. Biosens Bioelectron 26(2):859–862CrossRefPubMedGoogle Scholar
  29. 29.
    Jia H, Wang Z, Wang C, Chang L, Li Z (2014) Real-time fluorescence detection of Hg2+ ions with high sensitivity by exponentially isothermal oligonucleotide amplification. RSC Adv 4(19):9439–9444CrossRefGoogle Scholar
  30. 30.
    Xuan F, Luo X, Hsing I-M (2013) Conformation-dependent exonuclease III activity mediated by metal ions reshuffling on thymine-rich DNA duplexes for an ultrasensitive electrochemical method for Hg2+ detection. Anal Chem 85(9):4586–4593CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and ResourcesChongqing Technology and Business UniversityChongqingChina
  2. 2.State Key Laboratory of Environment-Friendly Energy MaterialSouthwest University of Science and TechnologyMianyangChina
  3. 3.School of Pharmaceutical SciencesWenzhou Medical UniversityZhejiangChina

Personalised recommendations

Cite article

Buy options