Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission
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The authors describe a molecular beacon-based fluorescent probe for the determination of the cancer drug bleomycin (BLM). The probe was tagged with DNA-templated silver nanoclusters (DNA-AgNCs) and guanine-rich sequences (GRSs) at two terminals serving as signal reporter with a loop. In the absence of the BLM-iron(II) complex [BLM-Fe(II)], the probe has a hairpin shape and displays strong fluorescence because the AgNCs are close to the GRSs. In the presence of the BLM-Fe(II) complex, it will selectively cleave the probe at the 5’-GC-3′ scission site of the loop. This displaces the AgNCs away from the GRSs and causes a decrease in fluorescence, best measured at excitation/emission wavelengths of 565/623 nm. This effect enables BLM to be detected with a detection limit as low as 33 pM, which was 1–3 orders of magnitude more sensitive than most of the previous reports. The probe was applied for the determination of BLM in spiked human serum samples, and excellent performance was achieved. In our perception, the method described here represents a promising tool for highly sensitive and specific analysis of BLM during cancer treatment.
KeywordsFluorescence DNA-templated synthesis Silver nanoclusters Bleomycin Guanine-rich sequence Antitumor drug DNA scission
This work is kindly supported by the National Natural Science Foundation of China (Nos. 21405094, 21775088, and 81403051), the Natural Science Foundation of Shandong Province (Nos. ZR2017MC037, ZR2017QB008), the Natural Science Foundation of Qinghai Province of China (2016-ZJ-955), and the Development Project of Qinghai Key Laboratory (No. 2017-ZJ-Y10), and the Open Funds of Shandong Key Laboratory of TCM Quality Control Technology of Shandong Academy of Sciences. We also thank Xiaoqing Qu for her good advice in the design of DNA.
Compliance with ethical standards
The authors declare that they have no competing interest.
- 5.Peng RQ, Sridhar S, Tyagi G, Phillips JE, Garrido R, Harris P, Burns L, Renteria L, Woods J, Chen L, Allard J, Ravindran P, Bitter H, Liang Z, Hogaboam CM, Kitson C, Budd DC, Fine JS, Bauer CMT, Stevenson CS (2013) Bleomycin induces molecular changes directly relevant to idiopathic pulmonary fibrosis: a model for “active” disease. PLoS One 8:e59348CrossRefPubMedPubMedCentralGoogle Scholar
- 8.D’Hondt M, Gevaert B, Wynendaele E, Spiegeleer BD (2016) Implementation of a single quad MS detector in routine QC analysis of peptide drugs. J. Pharmaceut Biomed 6:24–31Google Scholar