Three kinds of lateral flow immunochromatographic assays based on the use of nanoparticle labels for fluorometric determination of zearalenone
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Colloidal gold, quantum dots and polystyrene microspheres were used as labels in three kinds of lateral flow immunochromatographic assays (ICAs) for the detection of zearalenone (ZEN) in cereal samples. The assays allow ZEN to be quantified within 20 min. The LODs are 10 μg·L−1 of ZEN for the colloidal gold-based ICA, and 1 μg·L−1 for both the quantum dot and polystyrene microsphere based ICAs. The respective data are 60 μg·kg−1, 6 μg·kg−1 and 6 μg·kg−1, respectively, for spiked samples and cereals. Only minor cross-sensitivity occurred between ZEN and fusarium toxins, and no cross-sensitivity if found for aflatoxin B1, T-2 mycotoxin, ochratoxin A, deoxynivalenol, and fumonisin B1. LODs of the three assays are lower than the maximum limits of ZEN set by most standardization agencies.
KeywordsColloidal gold Quantum dots Polystyrene microspheres Immunochromatographic assay Cereal samples
The authors are grateful for providing language help from the American Journal Experts.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 5.FAO Agric (2003) In worldwide regulations for mycotoxins in food and feed in 2003. FAO Food and Nutration Paper, 81Google Scholar
- 6.CEC (2006) Setting maximum levels for certain contaminants in foodstuffs. Off J Eur Communities: Legis 364:5–24Google Scholar
- 11.Echarte JM, Fernández DC, Chiacchio CA, Leedham VMT (2014) Comparison of a validated LC/MS/MS method with a validated GC/MS method for the analysis of Zeranol and its related mycotoxin residues in bovine urine samples collected during Argentina's residue monitoring control program (2005-2012). J AOAC Int 97:1470–1475CrossRefGoogle Scholar
- 13.Duca RC, Bravin F, Delaforge M, Vladescu L, Badea IA, Criste RD (2009) Development of a new HPLC method used for determination of zearalenone and its metabolites in broiler samples. Influence of zearalenone on the nutritional properties of broiler meat. J Agric Food Chem 57:10497–10504CrossRefGoogle Scholar
- 19.Xu W, Qing Y, Chen S, Chen J, Qin Z, Qiu J, Li C (2017) Electrochemical indirect competitive immunoassay for ultrasensitive detection of zearalenone based on a glassy carbon electrode modified with carboxylated multi-walled carbon nanotubes and chitosan. Microchim Acta 184(9):3339–3347CrossRefGoogle Scholar
- 25.Fu X, Chu Y, Zhao K, Li J, Deng A (2017) Ultrasensitive detection of the β-adrenergic agonist brombuterol by a SERS-based lateral flow immunochromatographic assay using flower-like gold-silver core-shell nanoparticles. Microchim Acta 184(6):1711–1719. https://doi.org/10.1007/s00604-017-2178-3 CrossRefGoogle Scholar
- 28.Tang X, Li P, Zhang Q, Zhang Z, Zhang W, Jiang J (2017) Time-resolved fluorescence Immunochromatographic assay developed using two Idiotypic Nanobodies for rapid, quantitative, and simultaneous detection of aflatoxin and Zearalenone in maize and its products. Anal Chem 89(21):11520–11528CrossRefGoogle Scholar
- 29.Thouvenot D, Morfin RF (1983) Radioimmunoassay for Zearalenone and Zearalanol in human serum: production, properties and use of porcine antibodies. Appl Environ Microbiol 45:16–23Google Scholar
- 32.He Q, Peng H, Yang J, Xu Z, Fan C, Sun Y (2017) QuEChERS extraction followed by enzyme-linked immunosorbent assay for determination of deoxynivalenol and zearalenone in cereals. Food Agric Immunol:1–19. https://doi.org/10.1080/09540105.2017.1348491
- 35.Chen Y, Chen Q, Han M, Zhou J, Gong L, Niu Y, Zhang Y, He L, Zhang L (2016) Development and optimization of a multiplex lateral flow immunoassay for the simultaneous determination of three mycotoxins in corn, rice and peanut. Food Chem 213:478–484. https://doi.org/10.1016/j.foodchem.2016.06.116 CrossRefGoogle Scholar