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Additional effects of duodenojejunal bypass on glucose metabolism in a rat model of sleeve gastrectomy

  • Hiroomi TakayamaEmail author
  • Masayuki Ohta
  • Kazuhiro Tada
  • Kiminori Watanabe
  • Takahide Kawasaki
  • Yuichi Endo
  • Yukio Iwashita
  • Masafumi Inomata
Original Article
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Abstract

Purpose

Sleeve gastrectomy with duodenojejunal bypass (SG-DJB) is expected to become a popular procedure in East Asia. The aim of this study was to evaluate the effects of duodenojejunal bypass on glucose metabolism in a rat model of sleeve gastrectomy (SG).

Methods

Twenty-four Sprague–Dawley rats were divided into two groups: SG-DJB and SG alone. 6 weeks after surgery, body weight, feed intake, and metabolic parameters were measured, and oral glucose tolerance tests (OGTT) were performed. The mRNA expression of factors related to gluconeogenesis and glucose transport was evaluated using jejunal samples. Protein expression of factors with significantly different mRNA expression levels was evaluated using immunohistochemistry.

Results

Body weight and metabolic parameters did not significantly differ between the two groups. During the OGTT, the SG-DJB group showed an early increase in serum insulin followed by an early decrease in blood glucose compared with the SG group. Expression levels of glucose transporter 1 (GLUT1) and sodium-glucose cotransporter 1 (SGLT1) mRNA and protein in the alimentary limb (AL) were greater in the SG-DJB group than in the SG group.

Conclusions

The additional effects of duodenojejunal bypass on glucose metabolism after SG may be related to increased expression of GLUT1 and SGLT1 in the AL.

Keywords

Sleeve gastrectomy with duodenojejunal bypass Sleeve gastrectomy Glucose metabolism GLUT1 SGLT1 
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Notes

Acknowledgements

We would like to thank Ms. Yuiko Aso and Mayumi Wada for their technical assistance with the experiments.

Compliance with ethical standards

Conflict of interest

This work was supported by JSPS KAKENHI Grant Number JP16K10505. The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Hiroomi Takayama
    • 1
    Email author
  • Masayuki Ohta
    • 1
  • Kazuhiro Tada
    • 1
  • Kiminori Watanabe
    • 1
  • Takahide Kawasaki
    • 1
  • Yuichi Endo
    • 1
  • Yukio Iwashita
    • 1
  • Masafumi Inomata
    • 1
  1. 1.Department of Gastroenterological and Pediatric SurgeryOita University Faculty of MedicineYufuJapan

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