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Acta Diabetologica

, Volume 56, Issue 5, pp 581–592 | Cite as

Understanding the gut–kidney axis among biopsy-proven diabetic nephropathy, type 2 diabetes mellitus and healthy controls: an analysis of the gut microbiota composition

  • Sibei Tao
  • Lingzhi Li
  • Ling Li
  • Yuan Liu
  • Qian Ren
  • Min Shi
  • Jing Liu
  • Jing Jiang
  • Huichao Ma
  • Zhuo Huang
  • Zijing Xia
  • Jing Pan
  • Tiantian Wei
  • Yan Wang
  • Peiyun Li
  • Tian Lan
  • Xi Tang
  • Xiaoxi Zeng
  • Song Lei
  • Huairong Tang
  • Liang MaEmail author
  • Ping FuEmail author
Original Article
Part of the following topical collections:
  1. Gut Microbiome and Metabolic Disorders

Abstract

Aims

Type 2 diabetes mellitus (T2DM) has a rising prevalence and gut microbiota involvement is increasingly recognized. Diabetic nephropathy (DN) is a major complication of T2DM. The aim of the study was to understand the gut–kidney axis by an analysis of gut microbiota composition among biopsy-proven DN, T2DM without kidney disease, and healthy control.

Methods

Fecal samples were collected from 14 DNs, 14 age/gender-matched T2DMs without renal diseases (DM), 14 age and gender-matched healthy controls (HC) and household contacts (HH) of DM group. The microbiota composition was analyzed by 16sRNA microbial profiling approach.

Results

Substantial differences were found in the richness of gut microbiota and the variation of bacteria population in DM compared to HC, and DN compared to DM, respectively. DM could be accurately distinguished from age/gender-matched healthy controls by the variable of genus g_Prevotella_9 (AUC = 0.9), and DN patients could be accurately distinguished from age/gender-matched DM by the variables of two genera (g_Escherichia-Shigella and g_Prevotella_9, AUC = 0.86). The microbiota composition of HH group was close to that of HC group, and was different from DM group. Under the same diet, DM could be more accurately detected by the same genus (g_Prevotella_9, AUC = 0.92).

Conclusion

Gut microbiota composition was explored to be related to the occurrence of biopsy-proven DN from DM. DM could be distinguished from HC by detecting g_Prevotella_9 level in feces, while DN was different from DM by the variables of g_Escherichia-Shigella and g_Prevotella_9, which potentially contributed to the physiopathological diagnosis of DN from DM.

Keywords

Gut–kidney axis Gut microbiota Diabetic nephropathy Type 2 diabetes mellitus 

Notes

Funding

The study is supported by National Key Research & Development Program of China (2016YFC1305403), National Natural Science Foundation of China (81700634) and International cooperation project (2016HH0069) funded by Science and Technology Department of Sichuan Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval was approved by Biomedical Ethics Committee of West China Hospital of Sichuan University (No. 2016-273). All the procedures followed the Declaration of Helsinki principles.

Informed consent

All participants provided written informed consent before enrolment in the study.

Supplementary material

592_2019_1316_MOESM1_ESM.doc (166 kb)
Supplementary material 1 (DOC 166 KB)

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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2019

Authors and Affiliations

  • Sibei Tao
    • 1
  • Lingzhi Li
    • 1
  • Ling Li
    • 1
  • Yuan Liu
    • 2
  • Qian Ren
    • 1
  • Min Shi
    • 1
  • Jing Liu
    • 1
  • Jing Jiang
    • 1
  • Huichao Ma
    • 1
  • Zhuo Huang
    • 1
  • Zijing Xia
    • 1
  • Jing Pan
    • 1
  • Tiantian Wei
    • 1
  • Yan Wang
    • 1
  • Peiyun Li
    • 1
  • Tian Lan
    • 1
  • Xi Tang
    • 1
  • Xiaoxi Zeng
    • 3
  • Song Lei
    • 4
  • Huairong Tang
    • 2
  • Liang Ma
    • 1
    • 5
    Email author
  • Ping Fu
    • 1
    Email author
  1. 1.Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for GeriatricsWest China Hospital of Sichuan UniversityChengduChina
  2. 2.Chinese Health Service Management DepartmentWest China Hospital of Sichuan UniversityChengduChina
  3. 3.West China Biomedical Big Data CenterSichuan UniversityChengduChina
  4. 4.Department of PathologyWest China Hospital of Sichuan UniversityChengduChina
  5. 5.Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney DiseasesNational Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney DiseaseBeijingChina

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