The objective of the present study was to evaluate the effect of Eclipta alba (L.) and E. alba nanoparticles on haematological and hepatoprotective parameters in diethyl nitrosamine (DEN)–induced toxicity and hepatocarcinogenesis model in rats. Eighty-one Wistar albino rats were divided into four groups. Group I control (n = 19), group II DEN .01% in water (n = 19), group III (DEN+ raw E. alba 50 mg/kg BW/day) and group IV (DEN+ nano-E. alba 50 mg/kg BW/day). DEN group (II) showed a significant (P < 0.05) decrease in the haemoglobin (Hb), PCV, total erythrocyte and platelet value whereas total leucocyte count (TLC) and absolute neutrophil count showed a significant (P < 0.05) increase when compared with the sham control group. Thus, E. alba nanoparticle treatment was able to offer better protection as compared with the raw E. alba against DEN toxicity. Anaemic changes and thrombocytopenia were less in E. alba treatments. Nano-E. alba could be capable to alleviate the DEN toxicity.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Abdallah AM, Nasr AM, El-Deen N, Neamat-Allah ANF, Heba I, El-Aziz A (2018) Evaluation of the hematoprotective and hepato-renal protective effects of Thymus vulgaris aqueous extract on thermally oxidized oil-induced hematotoxicity and hepato-renal toxicity. Comp Clin Pathol 1:1–10
Abdallah AAM, Nasr El-Deen NAM, Neamat-Allah NAF, El-Aziz H (2019a) Evaluation of the hematoprotective and hepato-renal protective effects of Thymus vulgaris aqueous extract on thermally oxidized oil-induced hematotoxicity and hepato-renal toxicity. Comp Clin Pathol 26:1–11
Abdallah AAM, Nasr El-Deen NAM, Neamat-Allah NAF, El Aziz HIA (2019b) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Pathol 18:2747–2749
Chokotia LS, Vashistha P, Sironiya R, Matoli H (2013) Pharmacological activities of Eclipta alba (L.). Int J Res Dev Pharm Life Sci 2(4):499–502
Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM (2019a) The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Orient Pharm Exp Med 19:403–413
Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hossein MS (2019b) Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol 18:2866–2863
Gupta M, Kanti U, Palla K, Chandi C, Manikanda L, Senthil GP (2005) Anticancer activity of Indigofera aspalathoides and Wedelia calendulaceae in Swiss albino mice. Iran J Pharm Res 6(2):141–145
Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotoc ininduced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26:455–463
Jalavland AR, Zhaleh M, Gooran S, Zangeneh MM, Seyedi N, Zangeneh MR (2019) Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum R.M. Fritsch leaves rich in linolenic acid, methyl ester. J Photochem Photobiol B 192:103–112
Lal VK, Amit K, Prashant K, Kuldeep SY (2010) Screening of leaves and roots of Eclipta alba for hepatoprotective activity. Arch Appl Sci Res 2(1):86–94
Moradi R, Majid H, Almasi SAM, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol 17(65):47–60
Nivedita A, Singh SK, Singh JK (2014) Efficacy of Eclipta alba (L.) against sublethal dose of endosulfan induced biochemical and hematological alterations in Swiss albino mice. Int J Pharm Sci Rev Res 27(2):216–221
Pithayanukul P, Lapett B, Bavovada R, Pakmanee N, Suttisri R (2007) Inhibition of proteolytic and hemorrhagic activities by ethyl acetate extract of Eclipta prostrata against Malayan pit viper venom. Pharm Biol 45:282–288
Prakash KM, Naidu PVS, Muralidhar P (2011) Promising phytochemicals from medicinal plant Eclipta alba. Int J Pharm Technol 3(2):868–287
Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69:535–543
Singh B, Saxena AK, Chandan BK, Agarwal SG, Bhatia MS, Anand KK (1993) Hepapatoprotective effect of ethanolic extract of Eclipta alba on experimental liver damage. Phytother Res 7:154–158
Singh A, Kumar R, Nivedita JK, Singh T (2011) Radio-protective effect of Eclipta alba (L.) against radiation induced haematological changes in Swiss albino mice. J Nat Prod 4:177–177
Singh T, Sinha N, Singh A (2013) Biochemical and histopathological effects on liver due to acute oral toxicity of aqueous leaf extract of Eclipta alba on female Swiss albino mice. Indian J Pharmacol 45:61–65
Zangeneh MM (2018) Hematoprotective and nephroprotective properties of ethanolic extract of Anthemis odontostephana Boiss in Streptozotocin-induced diabetic mice. J Med Plants 17:47–60
Zangeneh MM, Zangeneh A, Tahvilia Z, Moradi R (2018a) Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice. Comp Clin Pathol 27:1119–1126
Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018b) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Pathol 26:1–11
Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018c) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Biol Sci 70:655–664
Zangeneh MM, Zangeneh A, Tahvilian R, Tehrani PR (2018d) Preclinical evaluation of hematoprotective and nephroprotective activities of Bellis perennis L aqueous extract on CCl4-induced renal injury in mice. Comp Clin Pathol 27:1557–1566
The authors thank Tamil Nadu Veterinary and Animal Sciences University, Chennai for providing necessary facilities to conduct this study.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Mugale, M.N., Balachandran, C. Haematological protective effect of raw extract and nano-Eclipta alba–treated rats in experimentally induced hepatocarcinogenesis. Comp Clin Pathol (2020). https://doi.org/10.1007/s00580-020-03092-1
- Eclipta alba nanoparticles