Antifungal and antileishmanial activities of fractions and isolated isoflavanquinones from the roots of Abrus precatorius
- 10 Downloads
Neglected tropical diseases hinder social and economic growth in many tropical areas. We report leishmanicidal activities of isoflavanquinones and antifungal potentials of fractions from methanol root extract of Abrus precatorius. Agar dilution method was used to determine antifungal properties while 96-well dilution protocol was adopted for antileishmanial activities. Ethyl acetate fraction of A. precatorius root extract showed moderate activity against M. canis and F. solani with percentage inhibition of 42.5% and 55.0% respectively while other fractions were inactive. Crude extract, n-hexane, and ethyl acetate fractions demonstrated potent antileishmanial activities with IC50 values of 22.20 ± 0.540 μg/mL, 19.35 ± 0.670 μg/mL, and 6.32 ± 0.001 μg/mL respectively, against L. major. The ethyl acetate fraction, which was the most active fraction, was subjected to successive column chromatography followed by preparative recycling HPLC in order to isolate the active constituents. Structures were established by HR-ESIMS, 1D and 2D NMR (1H, 13C, COSY, NOESY, HMBC, HSQC) spectroscopy. Two compounds were obtained and identified as isoflavanquinones: abruquinone A (1) and abruquinone B (2). Compounds 1 and 2 demonstrated significant (p < 0.05) antileishmanial activity against L. major (IC50 6.35 ± 0.005 μg/mL and 6.32 ± 0.008 μg/mL respectively) and L. tropica (IC50 6.29 ± 0.015 μg/mL and 6.31 ± 0.005 μg/mL, respectively). This appears to be the first report of antileishmanial activity of compounds 1 and 2 from the genus Abrus against cutaneous leishmaniasis and validates the use of A. precatorius in treating dermatology diseases.
KeywordsAbrus precatorius Antifungal Isoflavanquinones Leishmania Neglected tropical diseases
EEO acknowledges the World Academy of Sciences for the advancement of Science in Developing Countries (TWAS), Trieste, Italy, for ICCBS-TWAS Fellowship (2018) at the H.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, Pakistan.
EEO conceptualized and designed the research work; EEO carried out the assays, analyzed data, and wrote the article manuscript, MSA elucidated the chemical structures; MSA, ORO, and FDO proofread the article manuscript.
This research work was fully funded by the International Centre for Chemical and Biological Sciences, ICCBS, University of Karachi, Karachi, Pakistan and The World Academy of Science (TWAS) Trieste, Italy, for the advancement of Science in Developing Countries.
Compliance with ethical standards
The authors declare that they have no competing interests.
- Androula P, Helena CM (2010) Leishmaniasis, an emerging infection in travelers. Int J Inf Secur 14:e1032–e1039Google Scholar
- Aziz AR, Volodymyr S, Surendra KJ, Babu LT, Shabana IK, Melissa RJ, Ilias M (2011) Antiparasitic and antimicrobial isoflavanquinones from Abrus schimperi. Nat Prod Commun 6(11):1645–1650Google Scholar
- Kalpesh M, Mehta M, Mendpara N, Garmit S, Shah G (2010) Determination of antibacterial activity of MIC of crude extract of Abrus precatorius L. Bitotechnol Adv 10:2Google Scholar
- Kishur SC, Ramakant S, Pradeep SP, Vidyadhish AK (2012) Pharmacological activities of Abrus precatorius Linn – a review. Int J Ayurvedic Herb Med 2(2):336–348Google Scholar
- Song C, Hu Z (1998) Abruquinone A, B, D, E, F, and G from the root of Abrus precatorius. Acta Bot Sin 40:734–739Google Scholar