Glucose levels are stable in stored blood and plasma samples of cultured female African catfish (Clarias gariepinus)
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Glucose is utilized by fish as one of the sources of energy. It is a physiological parameter useful in the determination of the general health condition of cultured fish. The aim of this study was to determine the stability of plasma and blood glucose concentration, stored at different temperatures (4 °C and 30 °C) for 72 h. Two (2) milliliters of blood was collected via the caudal abdominal venipuncture of 10 female catfishes purchased. The fish were 8 months of age and were kept in plastic ponds at the Faculty of Veterinary Medicine, University of Nigeria. Blood was collected in blood tubes containing K2EDTA. Plasma was harvested from 1 mL of K2EDTA blood sample following centrifugation. Glucose concentration was determined using the Accu-Chek glucometer. Whole blood and plasma were analyzed immediately (0 h), and then after storage at 4 °C and 30 °C at 24 h, 48 h, and 72 h. Comparisons to the whole blood samples (0 h) stored at 30 °C revealed a significant (p < 0.05) decrease in blood glucose concentration from the 24th hour to the 72nd hour. At 4 °C, blood glucose concentration was stable for up to 72 h. Plasma glucose level was stable for 24 h, and significantly (p < 0.05) decreased from the 48th hour. When compared with the 0 h plasma glucose concentration at 4 °C, no significant (p > 0.05) differences in the 24-h, 48-h, and 72-h plasma glucose concentration were observed. Therefore, whole blood and plasma stored at 4 °C for 72 h and plasma stored at 30 °C for 24 h can be used for glucose determinations in the female African catfish.
KeywordsGlucometer Glucose Plasma Whole blood 30 °C 4 °C Female catfish
This study was supported by the personal contributions of all authors. No grant was made available for this study. We would like to thank Rebec farms and the Veterinary Clinical students on rotation in the Department of Veterinary Theriogenology and Reproductive diseases laboratory for their assistance.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The research was carried out in accordance with the Ethics and Regulations guiding the use of animals and animal products, as approved by the University of Nigeria Senate committee on Medical and Research ethics.
- Collicutt NB (2014) The effect of delayed serum separation and storage temperature on the rate of serum glucose concentration decline in alpacas, horses, dogs and sturgeon. MSc Thesis. The University of Georgia. Athens, GeorgiaGoogle Scholar
- Garrett RH, Grisham CM (2013) Biochemistry, 5th edn. Brooks/Cole, Cengage LearningGoogle Scholar
- Kamalam BS, Medale F, Panserat S (2016) Utilisation of dietary carbohydrates in farmed fishes: new insights on influencing factors, biological limitations and future strategies. Aquaculture. https://doi.org/10.1010/j.aquaculture.2016.02.007
- Nourrisson CL, Batisse M, Sapin V, Bouvier D (2010) Pseudo-hypoglycemia and hyperleukocytosis: a case report. Ann Biol Clin (Paris) 68(4):490–494Google Scholar
- Sacks DB (2008) Carbohydrates. In: Burtis CA, Ashwood ER, Bruns DE (eds) Tietz fundamentals of clinical chemistry, 6th edn. St. Louis, Saunders Elsevier, p 373Google Scholar
- Singh M, Pandya R, Chandra S, Sharma SK (2015) Stability of clinical chemistry and haematological analytes in preserved plasma and blood obtained from Wistar rats. Scand J Lab Anim Sci 41(6):1–6Google Scholar
- Stockham SL, Scott MA (2008) Fundamentals of veterinary clinical pathology, 2nd edn. Blackwell Publishing, IowaGoogle Scholar
- Young D, Bermes E, Haverstick D (2008) Separation and storage of specimens. In: Burtis CA, Ashwood ER, Bruns DE (eds) Tietz fundamentals of clinical chemistry. Saunders Elsevier, St. Louis, p 51Google Scholar