Biochemical and total hemocyte count profiles of long-clawed crayfish (Astacus leptodactylus) as a reference interval in southern Iran

  • Shirin Safaeian
  • Amin Gholamhosseini
  • Fahimeh Heidari
  • Hesamodin Kordestani
  • Saeed NazifiEmail author
Original Article


One of the narrow-clawed crayfish of the freshwater is named Astacus leptodactylus. As the only crayfish species native to Iranian waters, Astacus leptodactylus is commonly distributed in Iranian water resources. Crayfish is considered a suitable biological indicator of the quality of water, a measure for the assessment of ecology and toxicology (ecotoxicology), and a model organism for the determination of the impact of pollutants. Blood biochemical parameters are useful indicators used to assess the physiological conditions of aquatic animals’ response to stress, contamination, physiological, and ecological changes; for this to work, knowing the normal parameter levels is vital. This study was aimed to determine the normal interval of the biochemical parameters in these species in Iran, which appears to be necessary for future planning and studies, especially for ecotoxicological studies. Biochemical parameters analyzed in this study were glucose (11.37 ± 1.53 mg/dl), urea (5.58 ± 0.62 mg/dl), creatinine (0.21 ± 0.02 mg/dl), AST (7.54 ± 0.82 U/L), ALT (7.04 ± 0.97 U/L), ALP (380.44 ± 33.46 U/L), total bilirubin (0.45 ± 0.32 mg/dl), calcium (8.39 ± 1.15 mg/dl), phosphorus (2.35 ± 0.24 mg/dl), total protein (1.72 ± 0.16 g/dl), albumin (4.30 ± 0.2 g/dl), iron (128.79 ± 12.54micg/dl), and magnesium (1.84 ± 0.32 mg/dl). The total number of hemocyte in samples was calculated to be mean 5.3 × 104 per milliliter (n = 30, range 3.9 × 103–1.59 × 105).


Astacus leptodactylus Crayfish Hemolymph Biochemical analysis 



The authors would like to thank the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University, for their technical support of this study.

Funding information

This work received financial support from the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University (Grant no. 71-GR-VT-5).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Animal ethics

All animal experiments were approved by the State Committee on Animal Ethics, Shiraz University, Shiraz, Iran (IACUC no.: 4687/63). The recommendations of the European Council Directive (86/609/EC) of November 24, 1986, regarding the standards in the protection of animals used for experimental purposes, were also followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical Studies, School of Veterinary MedicineShiraz UniversityShirazIran

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