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Erythrocyte osmotic fragility and erythrocyte sizes for captive African rock pythons (Python sebae)

  • Henry Olanrewaju JegedeEmail author
  • Folashade H. Olaifa
  • Adakole S. Adah
  • Arimie Deborah Adah
  • Temidayo Olutayo Omobowale
Original Article
  • 17 Downloads

Abstract

The aim of the experiment was to determine the erythrocyte sizes and osmotic fragility index of the African rock python in a transitional zone. Ten African rock pythons (ARPs) were used as experimental animals. Blood samples were obtained from captive ARP by venepuncture of the ventral coccygeal vein. Erythrocyte counts, packed cell volume, mean corpuscular volume and erythrocyte sizes were determined. Erythrocytes obtained from each snake were added into saline solution of different NaCl concentrations of 0.1, 0.3, 0.5, 0.7 and 0.9 g/100 mL, respectively. Erythrocyte osmotic fragility index was determined spectrophotometrically. Mean erythrocyte size was 76.9 ± 10.2 μm and nucleus size was 9.1 ± 2.4 μm. The mean erythrocyte count obtained was 3.3 ± 0.6 × 1012/L, the mean packed cell volume obtained was 24.0 ± 2.9%, and the mean corpuscular volume was 76.4 ± 4.9 fl. The percentage haemolysis increased significantly (P < 0.05) at 0.3 g/100 mL NaCl concentration with the value of 79.9 ± 5.6%. The result of this finding adds to already available haematological data of the ARP and also showed that their erythrocyte may be extremely fragile. More research should be done on the effect of antioxidants in improving erythrocyte fragility of ARPs in the region.

Keywords

Erythrocytes Erythrocyte size Osmotic fragility Python sebae 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Veterinary Teaching HospitalUniversity of IlorinIlorinNigeria
  2. 2.Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary MedicineUniversity of IlorinIlorinNigeria
  3. 3.Department of Veterinary Medicine, Faculty of Veterinary MedicineUniversity of IlorinIlorinNigeria
  4. 4.Department of Veterinary Medicine, Faculty of Veterinary MedicineUniversity of IbadanIbadanNigeria

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