The reliability of platelet counts obtained by different methods following different storage times/conditions is not well defined. The aim was to evaluate temporal changes in platelet count of canine blood samples submitted to two thermal storage conditions using three techniques of platelet counting. Blood samples were obtained from 40 dogs aged between 6 months and 12 years. Blood samples were collected by jugular venipuncture, separated into two aliquots, and stored in EDTA tubes: one kept at room temperature (RT group) and the other kept under refrigeration (RE group). The platelet count was performed using the hemocytometer method, blood smear counting, and automated counting immediately after blood collection (M0) and 30 min and 1, 2, 4, 6, and 24 h later in both groups. The platelet parameters provided by the electronic counter were also obtained. There was a significant difference in platelet counts between the manual and automated methods when the samples were processed 24 h after sample collection. The refrigerated samples remained with closer platelet values of the previous samples for longer than those stored at room temperature. The analysis of platelet parameters loses its validity when the samples are stored for more than 6 h. The plaquetogram is reliable when the sample is processed up to 4 h after collection, regardless of the storage method. The timing of the platelet count should not exceed 6 h after collection for refrigerated samples and 4 h for samples stored at room temperature, for all counting methods.
This is a preview of subscription content, log in to check access.
Buy single article
Instant unlimited access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Abella NHG, Geffré A, Deshuillers PL et al (2014) Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using the XT-2000iV analyzer. Vet Clin Pathol 43:24–35
Clark P, Mogg TD, Tvedten HW, Korcal D (2002) Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer. Vet Clin Pathol 31:90–94
Coelho E (1981) Técnicas de estudo da coagulação, 3rd edn. Santos, São Paulo, p 121
Comar SR, Silva PH (2009) Determinação laboratorial e aplicação clínica dos parâmetros de volume plaquetário. RBAC 41(1):257–265
Farias MG, Bó SD (2008) Determinação do intervalo de referência para o volume plaquetário médio (VPM) utilizando o analisador hematológico Pentra 120 ABX. RBAC 40(1):39–41
Ferreira GS, Masson GCIH, Costa EDCP et al (2009) Plateletcrit, mean platelet volume and platelet distribution width: its expected values and correlation parameters in dogs from northern region of Brazil. In: World Small Animal Veterinary Association World Congress Proceedings. Universidade Federal Rural da Amazônia-UFRA, Belém
Gao XM, Moore XL, Liu Y, Wang XY, Han LP, Su Y, Tsai A, Xu Q, Zhang M, Lambert GW, Kiriazis H, Gao W, Dart AM, du XJ (2016) Splenic release of platelets contributes to increased circulating platelet size and inflammation after myocardial infarction. Clin Sci 130:1089–1104
Gerday E, Baer VL, Lambert DK (2009) Testing platelet mass versus platelet count to guide platelet transfusions in the neonatal intensive care unit. Transfusion 49:2034–2039
Golwala ZM, Shah H, Gupta N, Sreenivas NGV, Puliyel JM (2016) Mean platelet volume (MPV), platelet distribution width (PDW), platelet count and plateletcrit (PCT) as predictors of in-hospital paediatric mortality: a case-control study. Afr Health Sci 16:356–362
Gulcu E, Durmaz Y, Karabay O (2013) Effect of severe sepsis on platelet count and their indices. Afr Health Sci 13(2):333–338
Humann-Ziehank E, Ganter M (2012) Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics. Animal 6:1115–1123
Jackson SR, Carter JM (1993) Platelet volume: laboratory measurement and clinical application. Blood Rev 7:104–113
Jain NC (1986) The platelets: structural, biochemical and functional aspects. In: Schalm’s veterinary hematology, 4th edn. Lea & Febiger, Philadelphia, pp 1–4
Knoll JS, Rowell S (1996) Clinical hematology. In-clinic analysis, quality control, reference values and system selection. Veterinary Clinics of North America: Small Animal Practice, Philadelphia, pp 981–1002
Lassen ED, Weisner G (2007) Tecnologia Laboratorial em Medicina Veterinária. In: Thrall MA (ed) Hematology and veterinary clinical biochemistry. São Paulo, Editora Roca, pp 3–36
Matos JF, Carvalho MG, Dusse LMS et al (2008) O papel do RDW, da morfologia eritrocitária e de parâmetros plaquetários na diferenciação entre anemias microcíticas e hipocrômicas. Rev Bras Hematol Hemoter 30:463–469
Médaille C, Briend-Marchal A, Braun JP (2002) Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Vet Clin Pathol 35:18–23
Moreno A, Menke D (2002) Assessment of platelet numbers and morphology in the peripheral blood smear. Clin Lab Med 22:193–213
Norman EJ, Barron RC, Nash AS (2001) Prevalence of low automated platelet counts in cats: comparison with prevalence of thrombocytopenia based on blood smear estimation. Vet Clin Pathol 30:137–140
Olsen LH, Kristensen AT, Qvortrup K, Pedersen HD (2004) Comparison of manual and automated methods for determining platelet count in dogs with macrothombocitopenia. J Vet Diagn Investig 16:167–170
Pastor J, Cuenca R, Velarde R, Viñas L, Lavin S (1997) Evaluation of a hematology analyzer with canine and feline blood. Vet Clin Pathol 26:138–147
Prins MW, Van Leeuwen MW, Teske E (2009) Stability and reproducibility of advia 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin contente (CHr) in the diagnosis of iron deficiency. Tijdschr Diergeneeskd 134:272–278
Reardom DM, Hutchinson D, Preston FE, Trowbridge EA (1985) The routine measurement of platelet volume: a comparison of aperture impedance and flow cytometric systems. Clin Lab Haematol 7:251–257
Schäfers A, Meierhans S, Sauter-Louis C et al (2013) Reference values for haematological and clinical-chemical parameters in the dog. Tierarztl Prax Ausg K 41(3):163–172
Schwartz D, Sharkey L, Armstrong PJ, Knudson C, Kelley J (2014) Platelet volume and plateletcrit in dogs with presumed primary immune-mediated thrombocytopenia. J Vet Intern Med 28:1575–1579
Schweirgert A, Rezende FH, Fantoni DT, Moroz LR (2010) Avaliação da contagem plaquetária pelo contador automático QBC Vet Autoread® comparado com estimativa em esfregaço sanguíneo e contagem em hemocitômetro. Semin Cienc Agrar 31:1001–1008
Shi G, Morrell CN (2011) Platelets as initiators and mediators of inflammation at the vessel wall. Thromb Res 127:387–390
Smith JR, Smith KF, Brainard BM (2014) Platelet parameters from an automated hematology analyzer in dogs with inflammatory clinical diseases. Vet J 201:406–411
Souza AM, Pereira JJ, Campos SDE et al (2016) Platelet indices in dogs with thrombocytopenia and dogs with normal platelet counts. Arch Med Vet 48:27–281
Stokol T, Erb HN (2007) A comparison of platelet parameters in EDTA and citrate anticoagulated blood in dogs. Vet Clin Pathol 36:148–154
Tasker S, Cripps PJ, Mackin AJ (2001) Evaluation of methods of platelet counting in the cat. J Small Anim Pract 42:326–332
Thompson CB, Jakubowski JA (1988) The pathophysiology and clinical relevance of platelet heterogeneity. Blood 72:1–8
Topper MJ, Weller EG (2003) Hemostasis. In: Latimer KS, Mahaffey EA, Prasse KW (eds) Duncan & Prasse’s veterinary laboratory medicine: clinical pathology, 4th edn, pp 99–135
Yilmaz Z, Eralp O, Ilcol YO (2008) Evaluation of platelet count and its association with plateletcrit, mean platelet volume, and platelet size distribution width in a canine model of endotoxemia. Vet Clin Pathol 37:159–163
Zelmanovic D, Hetherington EJ (1998) Automated analysis of feline platelets in whole blood, including platelet count, mean platelet volume and activation state. Vet Clin Pathol 27:2–9
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for and use of animals were followed.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Jaguezeski, A.M., Volpato, J., de Lorenzi Cancelier, C.D. et al. Evaluation of time and temperature storage in platelet counts in blood samples of dogs. Comp Clin Pathol 29, 155–160 (2020). https://doi.org/10.1007/s00580-019-03046-2
- Automated counting
- Storage times