Advertisement

Comparative Clinical Pathology

, Volume 29, Issue 1, pp 127–133 | Cite as

Morphometric study of red blood cells in non-descriptive cattle with respect to age and sex

  • Ipsita DashEmail author
Original Article
  • 25 Downloads

Abstract

The present study was conducted to investigate the influence of age (calf, young and adult) and sex (female and castrated male) on the morphometry (mean length and mean breadth) of red blood cells (RBCs) of non-descriptive cattle. Blood samples were collected from each non-descriptive cattle with respect to age and sex by jugular venipuncture and collected in EDTA vials. Then, blood smears were prepared on grease-free microscopic slides then air-dried, fixed on methanol, and stained with Giemsa stain for morphometric study. The morphometric study was done with the help of the ocular micrometer and stage micrometer under 40X objective. Since there was no previous report on comparative morphometrical analyses of castrated males and female ND cattle of Odisha, an attempt has been taken to undertake this particular study. For both mean length and breadth, no significant difference was there among the different age groups of female cattle. Highly significant differences (p < 0.001) were observed between the mean length of RBCs of male calves with young castrated males and adult males. For mean breadth of RBCs, a highly significant difference (p < 0.01) was there between the male calves and male adults. A highly significant difference (p < 0.01) was there between the mean length of RBCs of the male calves with the mean length of RBCs of the young females. Therefore, age and sex have a profound effect on the morphometry of RBCs in non-descriptive cattle. Therefore, careful attention must be done in studying and interpretation of anemic conditions on the basis of size.

Keywords

Calves Youngs Adults Length Breadth 

Notes

Compliance with ethical standards

Ethical approval

The investigation followed guidelines for the care and use of animals.

Conflict of interest

The author declares that she has no conflict of interest.

References

  1. Aceňa MC, Garcia-Belenguer S, Garson M, Purroy A (1995) Modifications hematologiques at musculaires pendant la corrida chez le taureau de combat. Rev Méd Vét 146(4):277–282Google Scholar
  2. Adams R, Garry FB, Aldridge BM, Holland MD, Odde KG (1992) Hematologic values in newborn beef calves. Am J Vet Res 53(6):944–950PubMedGoogle Scholar
  3. Adilli N, Mohamed M, Omar B (2013) The influence of age, sex and altitude on the morphometry of red blood cells in bovine. Vet World 6(8):476–478.  https://doi.org/10.5455/vetworld.2013.476-478 CrossRefGoogle Scholar
  4. Adilli N, Mohamed M, Belabbas H, Achouri A (2014) Preliminary study of the influence of red blood cells size on the determinism of the breed in cattle. Vet Med Int 2014:1–4.  https://doi.org/10.1155/2014/429495 CrossRefGoogle Scholar
  5. Ahmed SA, Ahmed EA (2011) Behavioral responses of castrated buck kids at different ages by using different methods of castration. J Am Sci 7:200–209Google Scholar
  6. Aoki T, Ishii H (2012) Hematological and biochemical profiles in peripartum mares and neonatal foals (heavy draft horse). J Equine Vet Sci 32:170–176Google Scholar
  7. Barger AM (2010) Erythrocyte morphology. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary Hematology. Wiley-Blackwell Publishing Ltd, Ames, pp 144–151Google Scholar
  8. Brar RS, Sandhu HS, Singh A (2002) Veterinary clinical diagnosis by laboratory methods, 1st edn. Kalyani Publisher, Ludhiana, p 10Google Scholar
  9. Brun-Hansen HE, Kampen AH, Lund A (2006) Hematologic values in calves during the first six months of life. Vet Clin Pathol 35(2):182–187PubMedGoogle Scholar
  10. Carlson GP (1996) Clinical chemistry tests. In: Smith BP (ed) Large animal internal medicine, 2nd edn. Mosby Publisher, St. LouisGoogle Scholar
  11. Dash I, Mohanty PK (2015) Morphological features and influence of age and breed on the morphometry of red blood cells of female cattle. IJSR 4(2):259–264Google Scholar
  12. Dash I, Bhattacherjee A, Mohanty PK (2015) Hematological analysis of three breeds of cows. IJB 2(1):31–39Google Scholar
  13. Fisher AD, Crowe MA, Alonso de la varga ME, Enright WJ (1996) Effect of castration method and the provision of local anaesthesia on plasma cortisol, scrotal circumference, growth and feed intake of bull calves. J Anim Sci 74:2336–2343PubMedGoogle Scholar
  14. Fisher AD, Crowe MA, O’Nualláin EM, Monaghan ML, Prendiville DJ, O’Kiely P, Enright WJ (1997) Effects of suppressing cortisol following castration of bull calves on adrenocorticotropic hormone, in vitro interferon-γ production, leukocytes, acute-phase proteins, growth, and feed intake. J Anim Sci 75:1899–1908PubMedGoogle Scholar
  15. Fisher AD, Knight TW, Cosgrove GP, Death AF, Anderson CB, Duganzich DM et al (2001) Effects of surgical or banding castration on stress responses and behaviour of bulls. Aust Vet J 79(4):279–284PubMedGoogle Scholar
  16. George JW, Lane VM, Snipes JN (2008) Changes in bovine hematology reference intervals from 1965 to 2001. Vet Clin Pathol 36:313–315Google Scholar
  17. Harvey JW (2001) Atlas of veterinary hematology: blood and bone marrow of domestic animals. WB Saunders Company, Philadelphia, pp 1–40Google Scholar
  18. Harvey JW (2008) The erythrocyte: physiology metabolism and biochemical disorders. In: Kareko JJ, Harvey JW, Bruss ML (eds) Clinical biochemistry of domestic animals, 6th edn. Academic Press, San Diego, pp 173–240Google Scholar
  19. Harvey JW (2010) Erythrocyte biochemistry. In: Weiss DJ, Wardrop KJ (eds) Schlam’s Veterinary Hematology, 6th edn. Wiley-Blackwell Publishing Ltd., Ames, pp 131–135Google Scholar
  20. Harvey JW, Asquith RL, Mcnulty PK, Kivipelto J, Bauer JE (1984) Hematology of the foals up to one year old. Equine Vet J 16(4):347–353PubMedGoogle Scholar
  21. Kapale PM, Jagtap DG, Badukale DM, Sahatpure SK (2008) Haematological constituents of blood of Gaolao cattle. Vet World 1(4):113–114Google Scholar
  22. Kramer JW (2000) Normal hematology of cattle, sheep and goats. In: Feldman BF, Zinkl JG, Jain NC (eds) Schlam’s veterinary hematology, 5th edn. Lippincott Williams and Wilkins, Philadelphia, pp 1075–1084Google Scholar
  23. Ledieu D (2003) Prélèvements en cytologie. Dans: Encyclopédie vétérinarie. Edns Scientifiques et Médicales Elsevier. Biologie Clinique, pp 0030Google Scholar
  24. Lillie RD (1977) HJ Conn’s biological stains, 9th edn. The Williams and Wilkins Company, Baltimore, pp 606–607Google Scholar
  25. Lobo J, Youg-Shun See E, Biggs M, Pandit A (2015) An insight into morphometric descriptors of cell shape that pertain to regenerative medicine. J Tissue Eng Regn Med.  https://doi.org/10.1002/term.1994 PubMedGoogle Scholar
  26. Mariella J, Pirrone A, Gentilini F, Castagnetti C (2014) Hematologic and biochemical profiles in Standardbred mares during peripartum. Theriogenology 81(4):526–534PubMedGoogle Scholar
  27. Masoni F, Lagadic M, Plassiocrt G, Guigand Le, Wyers M (1985) Paramėters Hématologiques de la chėvre laitiėre Variations physiologiques chez l’ animal Sain autour de la mise-bas. Rec Méd Vét 161(1):41–49Google Scholar
  28. McGrath K, Palis J (2008) Ontogeny of erythropoiesis in the mammalian embryo. Curr Top Dev Biol 82:1–22PubMedGoogle Scholar
  29. Meinkoth JH, Clinkenbeard KD (2000) Normal hematology of the dog. In: Feldman BF, Zinkl JG, Jain NC (eds) Schalm’s veterinary hematology, 5th edn. Lippincott Williams and Wilkins, Philadelphia, pp 1057–1063Google Scholar
  30. Mohammad BF, Zghoul AL, Raidal AK, Abdelsalam R, Talafha Q, Omar A, Baniismail ZA (2008) Cellular and some biochemical changes in blood and peritoneal fluid constituents in Awassi lambs following elective castration. Am J Anim Vet Sci 3:23–27Google Scholar
  31. Mohri M, Sharifi K, Eidi S (2007) Hematology and serum biochemistry of Holstein dairy calves: age related changes and comparison with blood composition in adults. Res Vet Sci 83(1):30–39PubMedGoogle Scholar
  32. Molony V, Kent JE, Robertson IS (1995) Assessment of acute and chronic pain after different methods of castration of calves. Appl Anim Behav Sci 46:33–48Google Scholar
  33. Okabe J, Tajmla S, Yamato O et al (1996) Hemoglobin types, erythrocyte membrane skeleton and plasma iron concentration in calves with poikilocytosis. J Vet Med Sci 58:629–634PubMedGoogle Scholar
  34. Olaifa AK (2018) Comparison in haematological and biochemical changes in normal, acute and chronically castrated West African Dwarf goats. Int J Res Med Sci 6(5):1623–1627Google Scholar
  35. Oyeyemi MO, Olaifa AK, Onwuka SK, Akinloye AK, Utho OA (2000) The effects of bilateral orchidectomy on some serum enzymes and proteins in the West African Dwarf buck. Afr J Biomed Res 3:105–108Google Scholar
  36. Robertson IS, Kent JE, Molony V (1994) Effect of different methods of castration on behaviour and plasma cortisol in calves of three ages. Res Vet Sci 56:8–17PubMedGoogle Scholar
  37. Roy S, Roy M, Mishra S (2010) Hematological and biochemical profile during gestation period in Sahiwal cows. Vet World 3(1):26–28Google Scholar
  38. Sánchez-Corrales YE, Hartley M, Jv R, Marée AFM, Grieneisen V (2018) Morphometrics of complex cell shapes: lobe contribution elliptic Fourier analysis (LOCO-EFA). Educ Technol Res Dev 145:156778.  https://doi.org/10.1242/dev.156778 CrossRefGoogle Scholar
  39. Sastry GA (1983) Veterinary clinical pathology, 3rd edn. CBS Publishers and Distributors, Delhi, pp 1–30Google Scholar
  40. Schlam OW, Carlson GP (1982) Equine medicine and surgery: the blood and the blood forming organs, 3rd edn. American veterinary publication, BhubaneswarGoogle Scholar
  41. Shaikat AH, Hassan MM, Khan SH, Islam MN, Hoque MA, Bari MS, Hossain ME (2013) Hemato-biochemical profiles of indigenous goats (Capra hircus) at Chittagong, Bangladesh. Vet World 6(10):789–793Google Scholar
  42. Svoboda M, Eichlerova K, Horak V, Hradecký J (2005) Development of haematological indices in melanoma-bearing Liběchov minipigs. Acta Vet Brno 74(4):603–611Google Scholar
  43. Thompson DW (1917) On growth and form. Cambridge University Press, CambridgeGoogle Scholar
  44. Thrall MA (2012) Erythrocyte morphology. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds) Veterinary hematology and clinical chemistry, 2nd edn. Wiley-Blackwell A John Wiley and Sons, Inc., Publication, Ames, p 63Google Scholar
  45. Wood D, Quiroz-Rocha GF (2010) Normal hematology of cattle. In: Weiss DJ, Wordrop KJ (eds) Schalm’s veterinary hematology, 6th. Wiley-Blackwell Publishing Ltd, Ames, pp 829–835Google Scholar
  46. Zobra R, Ardu M, Niccolini S, Cubeddu F, Dimauro C, Bonelli P, Dedola C, Visco S, Parpaglia MLP (2011) Physical, hematological and biochemical responses to acute intense exercise in polo horses. J Equine Vet Sci 31:542–548Google Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.P.G. Department of ZoologyUtkal UniversityBhubaneswarIndia

Personalised recommendations