Advertisement

Effect of egg white and honey enema on ulcerative colitis in an animal model

  • 36 Accesses

Abstract

Egg and honey contain a variety of bioactive compounds that can influence pro- and anti-inflammatory pathways and have effects on oxidative stress. In this study, the effects of egg white and honey against acetic acid-induced colitis were investigated in rats. Forty adult rats were divided into five groups (n = 7), group I (normal saline), group II (egg white), group III (honey), group IV (Asacol® 100 mg/ml used as a positive control), and group V (combination of honey and egg white 50:50 v/v). Medications (2 cc/day) were administered rectally for a period of 6 days after induction of colitis using 2% acetic acid. Animals were killed on day 7 and colonic biopsies were taken for histopathological scoring. The concentration of malondialdehyde (MDA) and TNF-α was measured on the samples of the colon tissue that were frozen in liquid nitrogen. At 1 week, the histological damage was severe, and the MDA and TNF-α were high throughout the study without any treatment. Honey decreased the TNF-α and MDA tissue levels towards normal levels significantly (P < 0.05). Combination of honey and egg white was not better than honey alone as an anti-inflammatory agent. In the animal model of ulcerative colitis, rectal administration of honey significantly reduced the colonic inflammation. The present study indicates that honey is efficacious in the acetic acid-induced rat colitis model, but these results require further confirmation in human studies and determination of active ingredients.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 99

This is the net price. Taxes to be calculated in checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Almasaudi SB, Abbas AT (2017) Manuka honey exerts antioxidant and anti-Inflammatory activities that promote healing of acetic acid-induced gastric ulcer in rats. 2017:5413917. https://doi.org/10.1155/2017/5413917

  2. Ananthakrishnan AN (2015) Environmental risk factors for inflammatory bowel diseases: a review. Dig Dis Sci 60(2):290–298. https://doi.org/10.1007/s10620-014-3350-9

  3. Andersen CJ (2015) Bioactive egg components and inflammation. Nutrients 7(9):7889–7913. https://doi.org/10.3390/nu7095372

  4. Azim MK, Perveen H, Mesaik MA, Simjee SU (2007) Antinociceptive activity of natural honey in thermal-nociception models in mice. Phytother Res: PTR 21(2):194–197. https://doi.org/10.1002/ptr.2049

  5. Bilsel Y, Bugra D, Yamaner S, Bulut T, Cevikbas U, Turkoglu U (2002) Could honey have a place in colitis therapy? Effects of honey, prednisolone, and disulfiram on inflammation, nitric oxide, and free radical formation. Dig Surg 19(4):306–311; discussion 311-302. https://doi.org/10.1159/000064580

  6. D’Odorico A, Bortolan S, Cardin R, D’Inca R, Martines D, Ferronato A, Sturniolo GC (2001) Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease. Scand J Gastroenterol 36(12):1289–1294

  7. Erejuwa OO, Sulaiman SA, Ab Wahab MS (2012) Honey: a novel antioxidant. Molecules (Basel, Switzerland) 17(4):4400–4423. https://doi.org/10.3390/molecules17044400

  8. Hanauer SB (2006) Inflammatory bowel disease: epidemiology, pathogenesis, and therapeutic opportunities. Inflamm Bowel Dis 12(Suppl 1):S3–S9

  9. Huang W, Chakrabarti S, Majumder K, Jiang Y, Davidge ST, Wu J (2010) Egg-derived peptide IRW inhibits TNF-alpha-induced inflammatory response and oxidative stress in endothelial cells. J Agric Food Chem 58(20):10840–10846. https://doi.org/10.1021/jf102120c

  10. Jauregui-Amezaga A, Geerits A, Das Y, Lemmens B, Sagaert X, Bessissow T, Lobaton T, Ferrante M, Van Assche G, Bisschops R, Geboes K, De Hertogh G, Vermeire S (2017) A simplified Geboes Score for ulcerative colitis. J Crohns Colitis 11(3):305–313. https://doi.org/10.1093/ecco-jcc/jjw154

  11. Kornbluth A, Sachar DB (2004) Ulcerative colitis practice guidelines in adults (update): American College of Gastroenterology, Practice Parameters Committee. Am J Gastroenterol 99(7):1371–1385. https://doi.org/10.1111/j.1572-0241.2004.40036.x

  12. Kurutas EB, Cetinkaya A, Bulbuloglu E, Kantarceken B (2005) Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediat Inflamm 2005(6):390–394. https://doi.org/10.1155/mi.2005.390

  13. Larrick JW, Wright SC (1990) Cytotoxic mechanism of tumor necrosis factor-alpha. FASEB J 4(14):3215–3223

  14. Lee M, Kovacs-Nolan J, Yang C, Archbold T, Fan MZ, Mine Y (2009) Hen egg lysozyme attenuates inflammation and modulates local gene expression in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Agric Food Chem 57(6):2233–2240. https://doi.org/10.1021/jf803133b

  15. Loguercio C, D’Argenio G, Delle Cave M, Cosenza V, Della Valle N, Mazzacca G, del Vecchio Blanco C (1996) Direct evidence of oxidative damage in acute and chronic phases of experimental colitis in rats. Dig Dis Sci 41(6):1204–1211

  16. Lusby PE, Coombes AL, Wilkinson JM (2006) A comparison of wound healing following treatment with Lavandula x allardii honey or essential oil. Phytother Res: PTR 20(9):755–757. https://doi.org/10.1002/ptr.1949

  17. Mahgoub AA, el-Medany AH, Hagar HH, Sabah DM (2002) Protective effect of natural honey against acetic acid-induced colitis in rats. Trop Gastroenterol 23(2):82–87

  18. Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL (1989) Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology 96(3):795–803

  19. Murthy SN, Cooper HS, Shim H, Shah RS, Ibrahim SA, Sedergran DJ (1993) Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin. Dig Dis Sci 38(9):1722–1734

  20. Mythilypriya R, Shanthi P, Sachdanandam P (2007) Analgesic, antipyretic and Ulcerogenic properties of an indigenous formulation--Kalpaamruthaa. Phytother Res: PTR 21(6):574–578. https://doi.org/10.1002/ptr.2116

  21. Oz HS, Chen TS, McClain CJ, de Villiers WJ (2005) Antioxidants as novel therapy in a murine model of colitis. J Nutr Biochem 16(5):297–304. https://doi.org/10.1016/j.jnutbio.2004.09.007

  22. Prakash A, Medhi B, Avti PK, Saikia UN, Pandhi P, Khanduja KL (2008) Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats. Phytother Res: PTR 22(11):1511–1519. https://doi.org/10.1002/ptr.2523

  23. Sninsky CA, Safdi M, Katz S (2008) A new look at a mainstay ulcerative colitis therapy. Gastroenterol Hepatol 4(2 Suppl 4):1–12

  24. Takhshid MA, Ai J, Alavi SJ, Tavasoli AR (2007) Effects of diets enriched with fish oil, vitamin A and vitamin E on experimental ulcerative colitis in rat’s. Feyz J Kashan Univ Medi Sci 11(3):8–14

  25. Tanideh N, FAB AJ, Ashraf MJ, Kuhi O, Mehrabani D (2014a) The healing effect of strawberry extract on acetic acid-induced ulcerative colitis in rat. World Appl Sci J 31(3):281–288

  26. Tanideh N, Afaridi E, Mehrabani D, Azarpira N, Hosseinzadeh M, Amini M, Hosseini S (2014b) The healing effect of Berberis vulgaris in acetic acid-induced ulcerative colitis in rat. Middle-East J Sci Res 21:1288–1294

  27. Tanideh N, Jamshidzadeh A, Sepehrimanesh M, Hosseinzadeh M, Koohi-Hosseinabadi O, Najibi A, Raam M, Daneshi S, Asadi-Yousefabad SL (2016) Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats. Saudi J Gastroenterol 22(1):50–56. https://doi.org/10.4103/1319-3767.173759

  28. Young D, Fan MZ, Mine Y (2010) Egg yolk peptides up-regulate glutathione synthesis and antioxidant enzyme activities in a porcine model of intestinal oxidative stress. J Agric Food Chem 58(13):7624–7633. https://doi.org/10.1021/jf1011598

  29. Zheng L, Gao ZQ, Wang SX (2000) A chronic ulcerative colitis model in rats. World J Gastroenterol 6(1):150–152

Download references

Acknowledgments

We appreciate Sajad Daneshi for his technical assistant and the laboratory animal center of Shiraz University of Medical Sciences for provision and care of the animals and the RCC center in Shiraz University of Medical Sciences for English editing.

Author information

Correspondence to Hajar Khazraei.

Ethics declarations

Conflict of interest

The author declares that they have no conflict of interest.

Ethical approval

This article does contain studies with animals performed by the authors and the study protocol was approved by the Ethics Committee of Shiraz University of Medical Sciences according to the principles of laboratory animal care (no.16273).

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Hosseini, S.V., Abodrab, E.A.M., Khazraei, H. et al. Effect of egg white and honey enema on ulcerative colitis in an animal model. Comp Clin Pathol 29, 61–68 (2020). https://doi.org/10.1007/s00580-019-03023-9

Download citation

Keywords

  • Egg white
  • Honey
  • Ulcerative colitis
  • Surgery