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Toxicological evaluation of aqueous extract of different varieties of guava (Psidium guajava Linn) leaves

  • Leye Jonathan BabatolaEmail author
  • Ganiyu Oboh
  • Adedayo Oluwaseun Ademiluyi
Original Article
  • 5 Downloads

Abstract

Guava (Psidium guajava Linn) is a common fruit tree in Nigeria with different species; the leaves, fruits, and the bark are often used in folklore for different medicinal purposes. However, there is a dearth of information on safety assessment of the leaves extract of guava from different species. These studies conducted toxicological evaluation of white, red, and pink guava leaves extract in rats for 14 days. Oral administration of 50, 500, and 5000 mg/kg BWT was carried out; they were observed for toxicity signs hourly for the first 6 h after administration, and every day of the experimental period. Clinical indicators, body weight differences, mortality, and biochemical parameters were examined during the experimental period. There were no deaths, a slight body weight increase was observed in the rats, and no clinical sign was noticed throughout the period of the experiment. There were no significant changes in the rat tissue malondialdehyde (MDA) levels, and the serum liver indicator enzymes (aspartate aminotransferase (AST), alkaline phosphate (ALP), and alanine aminotransferase (ALT), bilirubin) and serum renal marker enzymes (creatinine, urea, uric acid, and blood urea nitrogen (BUN)) of rats administered when compared with the control. This entail that the NOAEL (no observed adverse effect level) estimate of white, red, and pink guava leaves extract is 50–5000 mg/kg.

Keywords

Guava Species Evaluation Oral administration NOAEL 

Notes

Acknowledgement

We would like to acknowledge Mr. Sunday Idowu Oyeleye of Functional Food and Nutraceutical Unit of the Department of Biochemistry, Federal University of Technology, Akure, for the technical support rendered during the course of this work, and also the 2018 IT students of the lab for their various assistance.

Funding

This work was funded by the authors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Ethical approval

This study conformed to international, national, and institutional guidelines for the use and care of animals.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiochemistryJoseph Ayo Babalola UniversityIleshaNigeria
  2. 2.Department of BiochemistryFederal University of TechnologyAkureNigeria

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