Concurrent infection of Leptospira icterohaemorrhagiae, Hepatozoon canis and Anaplasma phagocytophilum in a Labrador Retriever dog and its therapeutic management

  • Neeraj Thakur
  • G. E. ChethanEmail author
  • Akhilesh
  • C. Gaykwad
  • K. K. Reena
  • Anil Kumar
  • J. B. Rajesh
  • U. K. De
  • K. Mahendran
  • P. S. Banerjee
Case Report


In this report, concurrent infection of Leptospira interrogans serovar icterohaemorrhagiae, Hepatozoon canis and Anaplasma phagocytophilum in a Labrador Retriever dog and its successful therapeutic management have been described. A 3-year-old female dog was presented with a history of anorexia, fever, lethargy, weakness, vomiting, black-coloured faeces and yellowish urine. Clinical and laboratory examination revealed pyrexia, icteric mucous membranes, hepatomegaly, splenomegaly, anaemia, leukocytosis, thrombocytopaenia, hypoalbuminemia and hyperbilirubinemia. Hepatozoon canis and Anaplasma phagocytophilum infections were confirmed by blood smear examination. Leptospira icterohaemorrhagiae was confirmed by microscopic agglutination test (MAT). Animal was stabilised with polyionic fluids, and therapy was started with imidocarb dipropionate (6.6 mg/Kg, SC, single dose), doxycycline (5 mg/kg, PO, bid) for 28 days, N-acetyl cysteine (30 mg/Kg, IV, SID) for 5 days and fipronil (0.25%) spray for topical use. Supportive therapy was done with plasma volume expanders, multivitamins, haematinics and hepatoprotectants. The animal showed complete recovery after 2 months of therapy.


Leptospira icterohaemorrhagiae Hepatozoon canis Anaplasma phagocytophilum Imidocarb dipropionate Doxycycline 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human or animal participants performed by any of the authors. The article reports a clinical case presented to Referral Veterinary Polyclinic, Indian Veterinary Research Institute, Izatnagar. All protocols followed were as per the guidelines from the standard textbooks in Veterinary Medicine and were in compliance with ethical standards.


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© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of MedicineIndian Veterinary Research InstituteIzatnagarIndia
  2. 2.Department of Veterinary MedicineCVSc & AH, SelesihAizawlIndia
  3. 3.Division of ParasitologyIndian Veterinary Research InstituteIzatnagarIndia

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