Molecular detection of Theileria ovis (Apicomplexa: Theileriidae), Anaplasma ovis (Rickettsiales: Anaplasmataceae), and Mycoplasma sp. (Tenericutes: Mycoplasmataceae) from sheep blood in western Iran
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The present study was designed to detect tick-borne hemoparasitic agents in 35 blood samples collected from healthy sheep from Lorestan province located in western Iran. Sheep blood samples were examined only using molecular method. By polymerase chain reaction (PCR), 13 (65%), 3 (15%), and one samples (5%) were to be infected by Anaplasma ovis, Theileria ovis, and Mycoplasma species, respectively. One sample from each positive target PCR was sequenced, successfully. This study is an investigation on tick-borne pathogens of domestic sheep, and a Mycoplasma agent is reported for the first time in Iran.
KeywordsHemoparasitic diseases PCR Phylogenetic tree Sheep Iran
We would like to acknowledge the contribution to this ongoing study of the Lorestani villagers. Had it not been for their kind support and generosity, we would have not been able to garner data for this research work. We would also like to extend our gratitude and appreciation to the director and personnel of Razi Herbal Medicines Research Center and Lorestan University of Medical Sciences for their technical support.
This work was partially supported by a grant from the Lorestan University of Medical Sciences, Khorramabad, Iran.
Compliance with ethical standards
I hereby declare all ethical standards have been respected in preparation of the submitted article.
Conflict of interest
The authors declare that they have no conflict of interest.
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