Molecular detection of Theileria ovis (Apicomplexa: Theileriidae), Anaplasma ovis (Rickettsiales: Anaplasmataceae), and Mycoplasma sp. (Tenericutes: Mycoplasmataceae) from sheep blood in western Iran

  • Gholamreza Goudarzi
  • Majid Tavakoli
  • Behrouz Ezatpour
  • Habibollah Kooshki
  • Asadollah Hosseini-ChegeniEmail author
Original Article


The present study was designed to detect tick-borne hemoparasitic agents in 35 blood samples collected from healthy sheep from Lorestan province located in western Iran. Sheep blood samples were examined only using molecular method. By polymerase chain reaction (PCR), 13 (65%), 3 (15%), and one samples (5%) were to be infected by Anaplasma ovis, Theileria ovis, and Mycoplasma species, respectively. One sample from each positive target PCR was sequenced, successfully. This study is an investigation on tick-borne pathogens of domestic sheep, and a Mycoplasma agent is reported for the first time in Iran.


Hemoparasitic diseases PCR Phylogenetic tree Sheep Iran 



We would like to acknowledge the contribution to this ongoing study of the Lorestani villagers. Had it not been for their kind support and generosity, we would have not been able to garner data for this research work. We would also like to extend our gratitude and appreciation to the director and personnel of Razi Herbal Medicines Research Center and Lorestan University of Medical Sciences for their technical support.

Funding information

This work was partially supported by a grant from the Lorestan University of Medical Sciences, Khorramabad, Iran.

Compliance with ethical standards


I hereby declare all ethical standards have been respected in preparation of the submitted article.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Allison RW, Meinkoth JH (2010) Anemia caused by Rickettsia, Mycoplasma, and Protozoa. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Blackwell, Philadelphia, pp 199–210Google Scholar
  2. Bouckaert R, Heled J, Kühnert D, Vaughan T, Wu CH, Xie D, Suchard MA, Rambaut A, Drummond AJ (2014) BEAST 2: a software platform for Bayesian evolutionary analysis. PLoS Comput Biol 10:e1003537CrossRefGoogle Scholar
  3. Carter GR, Wise DJ (2004) Essentials of veterinary bacteriology and mycology. Iowa State Press, IowaGoogle Scholar
  4. de la Fuente J, Atkinson MW, Naranjo V, de Mera IGF, Mangold AJ, Keating KA, Kocan KM (2007) Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol 119:375–381CrossRefGoogle Scholar
  5. de la Fuente J, Estrada-Pena A, Venzal JM, Kocan KM, Sonenshine DE (2008) Overview: ticks as vectors of pathogens that cause disease in humans and animals. Front Biosci Volume(13):6938–6946CrossRefGoogle Scholar
  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  7. Friedhoff K (1997) Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. Parassitologia 39:99–109Google Scholar
  8. Ganta RR (2013) Anaplasmataceae: Anaplasma. In: McVey DS, Kennedy M, Chengappa MM (eds) Veterinary microbiology. John Wiley & Sons, Inc., Oxford, pp 302–305Google Scholar
  9. Gouy M, Guindon S, Gascuel O (2010) SeaView version 4 : a multiplatform graphical user interface for sequence alignment and phylogenetic tree building Mol Biol Evol 27:221–224Google Scholar
  10. Harvey J (2012) Veterinary hematology: a diagnostic guide and color atlas. Elsevier Saunders, MissouriGoogle Scholar
  11. Hashemi-Fesharki R (1997) Tick-borne diseases of sheep and goats and their related vectors in Iran. Parassitologia 39:115–117Google Scholar
  12. Heidarpour Bami M, Khazraiinia P, Haddadzadeh H, Kazemi B (2010) Identification of Theileria species in sheep in the eastern half of Iran using nested PCR-RFLP and microscopic techniques. Iran J Vet Res 11:262–266Google Scholar
  13. Kaufmann J (1996) Parasitic infections of domestic animals: a diagnostic manual. Springer, BaselCrossRefGoogle Scholar
  14. Kreier JP, Gothe R, Ihler GM, Krampitz HE, Mernaugh G, Palmer GH (1992) The hemotrophic bacteria: the families Bartonellaceae and Anaplasmataceae. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H (eds) The prokaryotes. Springer, New York, pp 3994–4022CrossRefGoogle Scholar
  15. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  16. Mehlhorn H, Schein E, Ahmed JS (1994) Theileria. In: Kreier JP (ed) Parasitic protozoa, vol 7. Academic Press, New York, pp 217–304CrossRefGoogle Scholar
  17. Neimark H (2001) Haemotrophic mycoplasmas. In: Service MW (ed) Encyclopedia of arthropod-transmitted infections of man and domesticated animals. CABI Publishing, New York, pp 208–213Google Scholar
  18. Nicholas R, Ayling R, McAuliffe L (2008) Mycoplasma diseases of ruminants. CABI, OxfordshireCrossRefGoogle Scholar
  19. Piesman J, Gage KL (2004) Bacterial and rickettsial diseases. In: Eldridge BF, Edman JD (eds) Medical entomology a textbook on public health and veterinary problems caused by arthropods. Kluwer Academic Publishers, Netherlands, pp 377–413Google Scholar
  20. Robertson JL, Teske E (2010) Disorders of the spleen. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Wiley-Blackwell, Iowa, pp 376–382Google Scholar
  21. SCI (2012) Agricultural census. Agricultural Statistics Office, Statistical Centre of Iran 2017Google Scholar
  22. SCI (2017) Agricultural census. Agricultural Statistics Office, Statistical Centre of IranGoogle Scholar
  23. Shaw MK (2002) Theileria development and host cell invasion. In: Shaw MK, Dobbelaere DAE, McKeever DJ (eds) World class parasites 3; Theileria. Springer, New York, pp 1–22Google Scholar
  24. Songer JG, Post KW (2005) Veterinary microbiology: bacterial and fungal agents of animal disease. Elsevier Saunders, MissouriGoogle Scholar
  25. Tabaei SJS, Spotin A, Pouriran R, Shahbazi A, Mamaghani AJ (2018) Molecular characterization of Theileria spp. in livestock and the first report on the occurrence of Theileria sp. OT3 in Iran. Acta Parasitol 63:515–521CrossRefGoogle Scholar
  26. Taylor MA, Coop RL, Wall RL (2007) Veterinary parasitology. Blackwell, OxfordGoogle Scholar
  27. Telmadarraiy Z, Oshaghi MA, Hosseini-Vasoukolaei N, Yaghoobi-Ershadi MR, Babamahmoudi F, Mohtarami F (2012) First molecular detection of Theileria ovis in Rhipicephalus sanguineus tick in Iran. Asian Pac J Trop Med 5:29–32CrossRefGoogle Scholar
  28. Yousefi A (2018) Phylogenetic analysis of Anaplasma marginale and Anaplasma ovis isolated from small ruminant based on MSP4 gene in western regions of Iran. Comp Clin Pathol 27:1161–1165CrossRefGoogle Scholar
  29. Yousefi A, Rahbari S, Shayan P, Sadeghi-dehkordi Z, Bahonar A (2017) Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran. Asian Pac J Trop Biomed 7:455–459CrossRefGoogle Scholar
  30. Zaeemi M, Haddadzadeh HR, Khazraiinia P, Kazemi B, Bandehpour M (2011) Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR–RFLP. Parasitol Res 108:837–843CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, School of MedicineLorestan University of Medical SciencesKhorramabadIran
  2. 2.Agricultural Research, Education and Extension Organization (AREEO)Lorestan Agricultural & Natural Resources Research CenterKhorramabadIran
  3. 3.Razi Herbal Medicines Research CenterLorestan University of Medical SciencesKhorramabadIran
  4. 4.Department of Medical Entomology and Vector Control, School of Public HealthTehran University of Medical SciencesTehranIran
  5. 5.Pol-e Dokhtar Higher Education CenterUniversity of LorestanPol-e DokhtarIran

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